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The map label for this gene is tuf [H]

Identifier: 150003401

GI number: 150003401

Start: 1118821

End: 1120005

Strand: Reverse

Name: tuf [H]

Synonym: BVU_0820

Alternate gene names: 150003401

Gene position: 1120005-1118821 (Counterclockwise)

Preceding gene: 150003402

Following gene: 150003400

Centisome position: 21.69

GC content: 41.86

Gene sequence:

>1185_bases
ATGGCTAAAGAGAAATTTGAACGTTCGAAACCGCACGTTAACATTGGTACTATCGGTCACGTTGACCACGGTAAAACCAC
TTTGACTGCTGCTATCACTACTGTGTTGGCTAAAAAAGGTCTTTCAGAATTGCGTTCTTTCGATTCTATCGACAACGCTC
CTGAAGAAAAAGAAAGAGGTATTACTATCAATACTTCTCACGTAGAATATCAAACTGCAAATCGTCACTATGCTCACGTT
GACTGTCCGGGTCACGCCGACTATGTAAAAAACATGGTTACAGGTGCTGCTCAGATGGATGGCGCAATTATTGTTTGTGC
TGCAACTGATGGTCCGATGCCTCAGACTCGTGAACACATCCTGTTGGCTCGTCAGGTAAACGTACCTCGTTTGGTTGTTT
TCTTGAACAAGTGTGATATGGTTGATGATGAAGAAATGTTGGAATTGGTAGAAATGGAAATGCGTGAATTGCTTTCATTC
TATGATTTCGATGGTGATAATACTCCTATTATTCGTGGTTCTGCATTAGGTGCTTTGAATGGTGTTCCTCAATGGGAAGA
TAAAGTAATGGAGTTGATGGATGCAGTTGATACTTGGATTCCATTGCCTCCGCGTGATATTGATAAACCTTTCTTGATGC
CTGTTGAAGACGTGTTCTCTATCACTGGTCGTGGTACTGTTGCTACAGGTCGTATCGAAGCTGGTATTATCCATGTAGGT
GACGAAGTTGAAATTCTTGGTTTGGGTGAAGATAAGAAATCAGTTGTTACTGGCGTTGAAATGTTCCGTAAATTATTGGA
TCAAGGTGAAGCTGGTGATAATGTTGGTTTGTTGTTGCGTGGTATCGACAAAAACGAAATCAAACGTGGTATGATTTTGT
GTAAGCCGGGTCAGGTTAAGGCTCACTCTAAGTTCAAAGCTGAGGTTTATATCTTGAAGAAAGAAGAAGGTGGTCGTCAT
ACTCCGTTCCATAACAAGTATCGTCCTCAGTTCTACTTGCGTACTATGGACTGTACAGGTGAAATCACTTTGCCGGAAGG
AACTGAAATGGTAATGCCTGGTGATAACGTAACTATTACAGTTGAGTTGATCTACCCGGTTGCATTGAATGTAGGTTTGC
GTTTTGCTATCCGTGAAGGTGGACGTACTGTAGGTGCAGGTCAGATTACAGAACTTCTTGACTAA

Upstream 100 bases:

>100_bases
CACTTCCTTGGTAAGGAAGAGGTCACGAGTTCAAGTCTCGTTAACAGCTCTTTGATAACGTAATTGCTGATTATTAATCA
AATAAATAACAAGTAAAGCT

Downstream 100 bases:

>100_bases
TCACTAGATAATTAATATGATCAGTTCTCGGTCGTAAGACACCGGGAACTGATTTTTACGGGAGTAGCTCAGTTGGTAGA
GCACCGGTCTCCAAAACCGG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHV
DCPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHILLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSF
YDFDGDNTPIIRGSALGALNGVPQWEDKVMELMDAVDTWIPLPPRDIDKPFLMPVEDVFSITGRGTVATGRIEAGIIHVG
DEVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMILCKPGQVKAHSKFKAEVYILKKEEGGRH
TPFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTITVELIYPVALNVGLRFAIREGGRTVGAGQITELLD

Sequences:

>Translated_394_residues
MAKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHV
DCPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHILLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSF
YDFDGDNTPIIRGSALGALNGVPQWEDKVMELMDAVDTWIPLPPRDIDKPFLMPVEDVFSITGRGTVATGRIEAGIIHVG
DEVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMILCKPGQVKAHSKFKAEVYILKKEEGGRH
TPFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTITVELIYPVALNVGLRFAIREGGRTVGAGQITELLD
>Mature_393_residues
AKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHVD
CPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHILLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSFY
DFDGDNTPIIRGSALGALNGVPQWEDKVMELMDAVDTWIPLPPRDIDKPFLMPVEDVFSITGRGTVATGRIEAGIIHVGD
EVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMILCKPGQVKAHSKFKAEVYILKKEEGGRHT
PFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTITVELIYPVALNVGLRFAIREGGRTVGAGQITELLD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.3451776649746, Blast_Score=467, Evalue=1e-132,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=31.0344827586207, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=31.0344827586207, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI4503475, Length=348, Percent_Identity=30.1724137931034, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI4503471, Length=452, Percent_Identity=28.5398230088496, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI54607086, Length=315, Percent_Identity=31.4285714285714, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI194018522, Length=349, Percent_Identity=28.9398280802292, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI194097354, Length=349, Percent_Identity=28.9398280802292, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194018520, Length=349, Percent_Identity=28.9398280802292, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI46094014, Length=359, Percent_Identity=27.8551532033426, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=28.0898876404494, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=36.0902255639098, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=73.3502538071066, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=73.5368956743003, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=389, Percent_Identity=29.305912596401, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI48994988, Length=282, Percent_Identity=30.4964539007092, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789108, Length=324, Percent_Identity=29.0123456790123, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788922, Length=335, Percent_Identity=25.9701492537313, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=50.3759398496241, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.2196382428941, Blast_Score=340, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=348, Percent_Identity=30.1724137931034, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=348, Percent_Identity=30.1724137931034, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17506081, Length=300, Percent_Identity=30.3333333333333, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32566303, Length=334, Percent_Identity=29.3413173652695, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=442, Percent_Identity=26.6968325791855, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=442, Percent_Identity=26.6968325791855, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=354, Percent_Identity=27.4011299435028, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17509919, Length=280, Percent_Identity=27.8571428571429, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=35.0649350649351, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=28.8135593220339, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=64.8989898989899, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325337, Length=346, Percent_Identity=31.7919075144509, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=346, Percent_Identity=31.7919075144509, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320863, Length=278, Percent_Identity=29.8561151079137, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322937, Length=352, Percent_Identity=25.2840909090909, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=26.5895953757225, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.0740740740741, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=240, Percent_Identity=28.3333333333333, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.4885496183206, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.4885496183206, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=392, Percent_Identity=49.7448979591837, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=27.3542600896861, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=27.3542600896861, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=27.7777777777778, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=27.7777777777778, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=27.7777777777778, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=27.7777777777778, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=31.1258278145695, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45550900, Length=354, Percent_Identity=27.4011299435028, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17137380, Length=346, Percent_Identity=26.878612716763, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=28.2142857142857, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.6245353159851, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28574573, Length=158, Percent_Identity=32.9113924050633, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582462, Length=159, Percent_Identity=30.8176100628931, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43564; Mature: 43432

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
ITINTSHVEYQTANRHYAHVDCPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHI
EEEECCEEEEEECCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSFYDFDGDNTPIIRGSALGALN
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHCC
GVPQWEDKVMELMDAVDTWIPLPPRDIDKPFLMPVEDVFSITGRGTVATGRIEAGIIHVG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECEEEEEEEEEC
DEVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMILCKPGQVK
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEECCCCCC
AHSKFKAEVYILKKEEGGRHTPFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTIT
CCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCEEEE
VELIYPVALNVGLRFAIREGGRTVGAGQITELLD
EEEEEEEHHCCCEEEEEECCCCEECCCHHHHHCC
>Mature Secondary Structure 
AKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
ITINTSHVEYQTANRHYAHVDCPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHI
EEEECCEEEEEECCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSFYDFDGDNTPIIRGSALGALN
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHCC
GVPQWEDKVMELMDAVDTWIPLPPRDIDKPFLMPVEDVFSITGRGTVATGRIEAGIIHVG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECEEEEEEEEEC
DEVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMILCKPGQVK
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEECCCCCC
AHSKFKAEVYILKKEEGGRHTPFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTIT
CCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCEEEE
VELIYPVALNVGLRFAIREGGRTVGAGQITELLD
EEEEEEEHHCCCEEEEEECCCCEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA