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The map label for this gene is purR [H]

Identifier: 148828376

GI number: 148828376

Start: 1830909

End: 1831919

Strand: Reverse

Name: purR [H]

Synonym: CGSHiGG_09905

Alternate gene names: 148828376

Gene position: 1831919-1830909 (Counterclockwise)

Preceding gene: 148828378

Following gene: 148828375

Centisome position: 97.07

GC content: 38.67

Gene sequence:

>1011_bases
ATGGCAACAATTAAAGATGTGGCAAAAATGGCTGGTGTGTCCACCACAACCGTATCTCACGTAATTAATAAAACCCGTTT
CGTTGCAAAAGATACGGAAGAAGCGGTGTTATCCGCAATTAAACAACTTAATTATTCCCCTAGTGCTGTGGCACGCAGTT
TAAAAGTCAATACCACCAAATCCATTGGTATGATTGTGACAACCAGTGAAGCTCCGTATTTTGCCGAAATAATCCATTCT
GTTGAAGAACATTGCTATCGACAAGGTTATTCTTTATTTTTGTGTAACACACAAAATGATCCTGAAAAAGTCAAAAATCA
TTTAGAAATGTTGGCAAAAAAAAGGGTCGATGGTTTGCTTGTAATGTGTTCTGAATATACTCAGGATTCATTGGATTTAC
TTTCATCATTTTCCACTATTCCAATGGTTGTAATGGATTGGGGGCCAAATGCCAATACCGATGTGATTGATGATCATAGT
TTTGATGGCGGTTATTTAGCAACCAAACATCTTATAGAGTGCGGTCATAAAAAAATCGGGATTATCTGCGGCGAACTCAA
TAAAACTACCGCAAGAACGCGTTATGAAGGCTTTGAAAAAGCAATGGAAGAAGCTAAATTAACAATCAATCCGTCTTGGG
TACTAGAGGGTGCATTTGAGCCTGAAGATGGCTATGAATGTATGAATCGTTTACTGACTCAAGAGGAATTACCAACCGCC
CTATTCTGCTGTAACGATGTGATGGCTCTCGGTGCAATATCAGCACTAACAGAAAAATGCTTGCGTGTGCCAGAGGATAT
GTCAATTATCGGCTATGATGATATTCACGCCTCACGTTTCTACGCACCACCATTAACCACCATTCATCAATCAAAACTAC
GTTTAGGCAGACAGGCAGTAAATATTCTGTTAGAACGAATTACTCACAAAGATGAAGGTGTTCAACAATATAGTCGTATC
GATATAACCCCTGAGCTTATCATTCGAAAATCCGTTAAATCGATTTTATAA

Upstream 100 bases:

>100_bases
TACGAAAAAATCACTTCTTTTTATAAAAAATTTGCAATAAAGATGACTAAATTCTCAACAAACCTTTATAATAGGAAAAA
TTTTTTAGGGATTTAAATAT

Downstream 100 bases:

>100_bases
ACTTAGCAAAAATAGACCGCACTTTTTAAACAATCCCTAAAAAGTGCGGTTCTTTTTTAGCTATTTTTTAGCTTTTCTTG
ATTATTTTCAAAATTTCTCT

Product: DNA-binding transcriptional repressor PurR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTKSIGMIVTTSEAPYFAEIIHS
VEEHCYRQGYSLFLCNTQNDPEKVKNHLEMLAKKRVDGLLVMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHS
FDGGYLATKHLIECGHKKIGIICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEELPTA
LFCCNDVMALGAISALTEKCLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAVNILLERITHKDEGVQQYSRI
DITPELIIRKSVKSIL

Sequences:

>Translated_336_residues
MATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTKSIGMIVTTSEAPYFAEIIHS
VEEHCYRQGYSLFLCNTQNDPEKVKNHLEMLAKKRVDGLLVMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHS
FDGGYLATKHLIECGHKKIGIICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEELPTA
LFCCNDVMALGAISALTEKCLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAVNILLERITHKDEGVQQYSRI
DITPELIIRKSVKSIL
>Mature_335_residues
ATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTKSIGMIVTTSEAPYFAEIIHSV
EEHCYRQGYSLFLCNTQNDPEKVKNHLEMLAKKRVDGLLVMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHSF
DGGYLATKHLIECGHKKIGIICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEELPTAL
FCCNDVMALGAISALTEKCLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAVNILLERITHKDEGVQQYSRID
ITPELIIRKSVKSIL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=56.5868263473054, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=48.1927710843374, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1790369, Length=336, Percent_Identity=33.0357142857143, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=35.6164383561644, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1786540, Length=304, Percent_Identity=32.5657894736842, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=29.9674267100977, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1789068, Length=292, Percent_Identity=28.7671232876712, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.6666666666667, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789456, Length=316, Percent_Identity=29.1139240506329, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=25.8928571428571, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=26.6666666666667, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790715, Length=317, Percent_Identity=25.8675078864353, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994940, Length=305, Percent_Identity=25.5737704918033, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786268, Length=306, Percent_Identity=22.8758169934641, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790192, Length=244, Percent_Identity=25.8196721311475, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37516; Mature: 37384

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTK
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SIGMIVTTSEAPYFAEIIHSVEEHCYRQGYSLFLCNTQNDPEKVKNHLEMLAKKRVDGLL
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
VMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHSFDGGYLATKHLIECGHKKIG
EEEHHHCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
IICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEELPTA
EEECCCCCHHHHHHHHHHHHHHHHCEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCHH
LFCCNDVMALGAISALTEKCLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NILLERITHKDEGVQQYSRIDITPELIIRKSVKSIL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
ATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTK
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SIGMIVTTSEAPYFAEIIHSVEEHCYRQGYSLFLCNTQNDPEKVKNHLEMLAKKRVDGLL
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
VMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHSFDGGYLATKHLIECGHKKIG
EEEHHHCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
IICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEELPTA
EEECCCCCHHHHHHHHHHHHHHHHCEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCHH
LFCCNDVMALGAISALTEKCLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NILLERITHKDEGVQQYSRIDITPELIIRKSVKSIL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA