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The map label for this gene is ackA [H]

Identifier: 148828354

GI number: 148828354

Start: 1799277

End: 1800482

Strand: Reverse

Name: ackA [H]

Synonym: CGSHiGG_09740

Alternate gene names: 148828354

Gene position: 1800482-1799277 (Counterclockwise)

Preceding gene: 148828357

Following gene: 148828353

Centisome position: 95.41

GC content: 39.97

Gene sequence:

>1206_bases
ATGTCAAAACTTGTTCTTATCCTTAACTGTGGTAGTTCTTCACTCAAATTTGCAATTCTTGATCCAGCAACAGGGGAAGA
AAAATTATCTGGCTTAGCCGAAGCATTTTTCTTACCAGAAGCACGTATCAAATGGAAACTCAATGGAGAGAAAGGTAACG
CAGATTTAGGCGCAGGTGCAGCGCATACTGAAGCACTTAATTTTATCGCATCTAACATTCTGAATGATGAGCTTAAAAAT
TCTATCGCGGCAATTGGTCATCGTATCGTTCACGGTGGCGAAAAATACACTCAATCTGTCATCGTAACAGATGAAGTTGT
TAAAGGAATTGAAGATGCTGCACAATTTGCGCCACTTCACAACCCTGCTCACTTAATTGGTATTCGCGAAGCATTCAAGG
CATTCCCACACTTAAAAGATAAAAACGTGGTAGTTTTTGATACAGCATTCCACCAAACTATGCCTGAAGAAGCCTTCCTT
TATGCCCTTCCATACTCTTTATATAAAGAACACGGCGTACGTCGCTATGGTGCACACGGCACAAGTCACTACTTCGTTTC
TCGTGAAGTCGCTGAATATGTAGGCAAACCTGCAGATCAAGTAAATGCTATTATTTGTCACTTAGGTAATGGTGGTTCTG
TTTCTGTTGTGCGTAACGGTCAATGTATTGATACTTCAATGGGCTTAACTCCGCTAGAAGGTTTAGTAATGGGTACGCGT
TGTGGCGATATTGACCCTGCAATCGTATTCTACCTATACAAAACACTAGGTATGTCTATGGATCAAATCGAAGAGACTTT
AGTGAAAAAATCGGGTCTTTTAGGTTTAACTGAAGTAACAAGCGACTGCCGTTATGCTGAAGATAACTACGATGATGAAT
CTAAACCAGAAACAAAACGCGCTTTAAATGTTTATAGCTATCGTTTAGCGAAATACATTGGTGCTTATATGGCTGTGTTG
GGCGATGATCACTTAGATGCAATTGCATTCACTGGTGGTATTGGCGAAAACTCAGCGCACGTTCGTGAATTAGCCTTAAA
TCACTTAAAATTATTCGGCATTAAAATCGATCACGAACGAAATCTTGCCACACGTTTTGGTAAAGATGGTGTAATCACCA
CTGATGATTCCGCATTTAAAGCAATTGTTCTTCCAACTAACGAAGAATTGGTTATCGCACAAGATACCGCAAAACTTTGT
TTCTAA

Upstream 100 bases:

>100_bases
TGAATTTATTTTATTCATCAAAAGGTGTATTCTATGTCTGATTTTTCAAACCATCTTAGATGATGGATTTCTATTAACCT
CAATCAAATATAGGGTTCCT

Downstream 100 bases:

>100_bases
TTTAACAACAAGCTGCCGAATTTTTCGGCAGTTTTTATTTATAAAATATACATATTAAGGTTTAACTATGTCTCGCACGA
TTATTCTTATCCCTGTAAGT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGAAHTEALNFIASNILNDELKN
SIAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLHNPAHLIGIREAFKAFPHLKDKNVVVFDTAFHQTMPEEAFL
YALPYSLYKEHGVRRYGAHGTSHYFVSREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTR
CGDIDPAIVFYLYKTLGMSMDQIEETLVKKSGLLGLTEVTSDCRYAEDNYDDESKPETKRALNVYSYRLAKYIGAYMAVL
GDDHLDAIAFTGGIGENSAHVRELALNHLKLFGIKIDHERNLATRFGKDGVITTDDSAFKAIVLPTNEELVIAQDTAKLC
F

Sequences:

>Translated_401_residues
MSKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGAAHTEALNFIASNILNDELKN
SIAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLHNPAHLIGIREAFKAFPHLKDKNVVVFDTAFHQTMPEEAFL
YALPYSLYKEHGVRRYGAHGTSHYFVSREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTR
CGDIDPAIVFYLYKTLGMSMDQIEETLVKKSGLLGLTEVTSDCRYAEDNYDDESKPETKRALNVYSYRLAKYIGAYMAVL
GDDHLDAIAFTGGIGENSAHVRELALNHLKLFGIKIDHERNLATRFGKDGVITTDDSAFKAIVLPTNEELVIAQDTAKLC
F
>Mature_400_residues
SKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGAAHTEALNFIASNILNDELKNS
IAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLHNPAHLIGIREAFKAFPHLKDKNVVVFDTAFHQTMPEEAFLY
ALPYSLYKEHGVRRYGAHGTSHYFVSREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTRC
GDIDPAIVFYLYKTLGMSMDQIEETLVKKSGLLGLTEVTSDCRYAEDNYDDESKPETKRALNVYSYRLAKYIGAYMAVLG
DDHLDAIAFTGGIGENSAHVRELALNHLKLFGIKIDHERNLATRFGKDGVITTDDSAFKAIVLPTNEELVIAQDTAKLCF

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=69, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=42.1717171717172, Blast_Score=308, Evalue=3e-85,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43754; Mature: 43623

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGA
CCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
AHTEALNFIASNILNDELKNSIAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEHHHHHHHHHHHHHHCCCC
NPAHLIGIREAFKAFPHLKDKNVVVFDTAFHQTMPEEAFLYALPYSLYKEHGVRRYGAHG
CCHHHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCCCCEEEECCHHHHHHCCCEECCCCC
TSHYFVSREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTR
CCEEEHHHHHHHHHCCCHHHCCEEEEECCCCCEEEEEECCEEEECCCCCCCHHHEEEECC
CGDIDPAIVFYLYKTLGMSMDQIEETLVKKSGLLGLTEVTSDCRYAEDNYDDESKPETKR
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCHHHH
ALNVYSYRLAKYIGAYMAVLGDDHLDAIAFTGGIGENSAHVRELALNHLKLFGIKIDHER
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCC
NLATRFGKDGVITTDDSAFKAIVLPTNEELVIAQDTAKLCF
CHHHHCCCCCCEECCCCCEEEEEECCCCCEEEEECCHHHCC
>Mature Secondary Structure 
SKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGA
CCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
AHTEALNFIASNILNDELKNSIAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEHHHHHHHHHHHHHHCCCC
NPAHLIGIREAFKAFPHLKDKNVVVFDTAFHQTMPEEAFLYALPYSLYKEHGVRRYGAHG
CCHHHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCCCCEEEECCHHHHHHCCCEECCCCC
TSHYFVSREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTR
CCEEEHHHHHHHHHCCCHHHCCEEEEECCCCCEEEEEECCEEEECCCCCCCHHHEEEECC
CGDIDPAIVFYLYKTLGMSMDQIEETLVKKSGLLGLTEVTSDCRYAEDNYDDESKPETKR
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCHHHH
ALNVYSYRLAKYIGAYMAVLGDDHLDAIAFTGGIGENSAHVRELALNHLKLFGIKIDHER
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCC
NLATRFGKDGVITTDDSAFKAIVLPTNEELVIAQDTAKLCF
CHHHHCCCCCCEECCCCCEEEEEECCCCCEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA