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The map label for this gene is murE [H]

Identifier: 148828293

GI number: 148828293

Start: 1728445

End: 1729911

Strand: Direct

Name: murE [H]

Synonym: CGSHiGG_09325

Alternate gene names: 148828293

Gene position: 1728445-1729911 (Clockwise)

Preceding gene: 148828292

Following gene: 148828294

Centisome position: 91.59

GC content: 38.45

Gene sequence:

>1467_bases
ATGAAAAAACTCACCGCACTTTTTAATTTGCCTGAATTAAAGAATGATATAGAACTCCATAATATGGTGTTAGATAGCCG
TAAGGTTAAAGCTGGCGATCTTTTTGTGGCGATAAAAGGTCATCAGGTGGATGGAAATCAATTTATTGATTCTGCTCTTC
ATTCTGGTGCGAGTGCGGTGGTTTCTGAGACAGAATTATCTAGCGAGCATTTAACTGTAGAGTTTATTGGGAATGTTCCC
GTAGTGAAATATCATCAACTTGCACGTCATCTTTCGTCCTTAGCTGATGTTTTTTATGATTCGCCCTCTAAAAATTTAAC
CCTTGTTGGCGTTACAGGAACAAATGGCAAAACCACTATTTCTCAATTATTAGCGCAATGGGCGGAATTATTGGGGCATC
GTGCTGCTGTGATGGGAACCATTGGTAATGGACTTTTTAGGCAAATTGTAGAAGCTAAAAATACGACAGGTTCAGCAGTA
GAAATTCAGTCATCTCTTTCAACTTTCAAACACGCAGGTGCAGATTTTACCTCTATTGAAGTTTCATCACACGGTTTGGC
GCAGCATCGTGTAGAAGCCTTGCATTTTAAAGCAGCAATTTTCACGAATTTAACCCGTGATCATCTAGATTATCATCAAT
CTATGGAAAATTATGCTGCGGCGAAGAAACGCTTGTTCACTGAATTAGATACCCAAATTAAAGTGATTAATGCTGATGAT
GAAATTGGATACCAATGGCTAACTGAACTACCTGATGCTATTGCCGTAAGTATGAATGCGGATTTTAAAGTAGGTTCACA
CCAATGGATGAAAGCAATAAATATCCATTATCATTTTAAAGGTGCAGATATTACTTTTGAATCTAGCTGGGGTAATGGTG
TTTTGCATAGCCCATTAATTGGTGCTTTTAATGTAAGTAATTTATTATTAGTAATGACCACGTTGTTATCGTTTGGTTAC
CCATTGGAAAATTTACTCGCTACGGCGAAATCTTTAAAAGGAGTATGTGGAAGAATGGAAATGATTCAATATCCAAATAA
ACCAACCGTTATTGTAGATTATGCGCATACACCAGATGCGTTAGAAAAAGCGTTGATTGCTGCGCGTGAACATTGCCAAG
GCGAATTATGGTGCATTTTTGGTTGTGGCGGAGACCGTGATAGAGGCAAACGTCCGTTAATGGCACAGATTGCAGAGCAG
TTTGCTGAAAAGATTATTGTGACAAAAGATAATCCACGAACAGAACCACAAAGCCAAATTGAAGCAGATATTGTCGCTGG
CTTTAAAAATATGGAAAAAGTGGGGATTATTCCTGATCGCGCACAGGCAATCCAGTTTGCGATTGAAAGTGCGGTAGAAA
ATGACGTGATTTTAATTGCTGGAAAGGGTCACGAGCATTATCAAATTATTGGTTCGGAAGTTGTGCATTTTTCCGACCAA
GAAATTGCACTTGATTTCTTAAAATAA

Upstream 100 bases:

>100_bases
GCAAGATGCTGAAGCAGCTGAAAACACAACAACGAAAAGTGCAAAACGTATTGTTTATATTGGCGAACACAAGAATCAAA
AAGTGAATTAAGGAAAAATT

Downstream 100 bases:

>100_bases
TAATCACTCAATTATGATTAAACTCTCTACTGTACAACTTGCACAGATTCTTCAAACTAAATTAATTGGCGATGAAAATG
TGCAAGTTGAAGAAATTAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKKLTALFNLPELKNDIELHNMVLDSRKVKAGDLFVAIKGHQVDGNQFIDSALHSGASAVVSETELSSEHLTVEFIGNVP
VVKYHQLARHLSSLADVFYDSPSKNLTLVGVTGTNGKTTISQLLAQWAELLGHRAAVMGTIGNGLFRQIVEAKNTTGSAV
EIQSSLSTFKHAGADFTSIEVSSHGLAQHRVEALHFKAAIFTNLTRDHLDYHQSMENYAAAKKRLFTELDTQIKVINADD
EIGYQWLTELPDAIAVSMNADFKVGSHQWMKAINIHYHFKGADITFESSWGNGVLHSPLIGAFNVSNLLLVMTTLLSFGY
PLENLLATAKSLKGVCGRMEMIQYPNKPTVIVDYAHTPDALEKALIAAREHCQGELWCIFGCGGDRDRGKRPLMAQIAEQ
FAEKIIVTKDNPRTEPQSQIEADIVAGFKNMEKVGIIPDRAQAIQFAIESAVENDVILIAGKGHEHYQIIGSEVVHFSDQ
EIALDFLK

Sequences:

>Translated_488_residues
MKKLTALFNLPELKNDIELHNMVLDSRKVKAGDLFVAIKGHQVDGNQFIDSALHSGASAVVSETELSSEHLTVEFIGNVP
VVKYHQLARHLSSLADVFYDSPSKNLTLVGVTGTNGKTTISQLLAQWAELLGHRAAVMGTIGNGLFRQIVEAKNTTGSAV
EIQSSLSTFKHAGADFTSIEVSSHGLAQHRVEALHFKAAIFTNLTRDHLDYHQSMENYAAAKKRLFTELDTQIKVINADD
EIGYQWLTELPDAIAVSMNADFKVGSHQWMKAINIHYHFKGADITFESSWGNGVLHSPLIGAFNVSNLLLVMTTLLSFGY
PLENLLATAKSLKGVCGRMEMIQYPNKPTVIVDYAHTPDALEKALIAAREHCQGELWCIFGCGGDRDRGKRPLMAQIAEQ
FAEKIIVTKDNPRTEPQSQIEADIVAGFKNMEKVGIIPDRAQAIQFAIESAVENDVILIAGKGHEHYQIIGSEVVHFSDQ
EIALDFLK
>Mature_488_residues
MKKLTALFNLPELKNDIELHNMVLDSRKVKAGDLFVAIKGHQVDGNQFIDSALHSGASAVVSETELSSEHLTVEFIGNVP
VVKYHQLARHLSSLADVFYDSPSKNLTLVGVTGTNGKTTISQLLAQWAELLGHRAAVMGTIGNGLFRQIVEAKNTTGSAV
EIQSSLSTFKHAGADFTSIEVSSHGLAQHRVEALHFKAAIFTNLTRDHLDYHQSMENYAAAKKRLFTELDTQIKVINADD
EIGYQWLTELPDAIAVSMNADFKVGSHQWMKAINIHYHFKGADITFESSWGNGVLHSPLIGAFNVSNLLLVMTTLLSFGY
PLENLLATAKSLKGVCGRMEMIQYPNKPTVIVDYAHTPDALEKALIAAREHCQGELWCIFGCGGDRDRGKRPLMAQIAEQ
FAEKIIVTKDNPRTEPQSQIEADIVAGFKNMEKVGIIPDRAQAIQFAIESAVENDVILIAGKGHEHYQIIGSEVVHFSDQ
EIALDFLK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=463, Percent_Identity=55.7235421166307, Blast_Score=497, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53712; Mature: 53712

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTALFNLPELKNDIELHNMVLDSRKVKAGDLFVAIKGHQVDGNQFIDSALHSGASAV
CCCHHHHHCCCCHHHHHHHHHHHHCCCCEECCCEEEEEECCCCCCHHHHHHHHHCCHHHH
VSETELSSEHLTVEFIGNVPVVKYHQLARHLSSLADVFYDSPSKNLTLVGVTGTNGKTTI
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHH
SQLLAQWAELLGHRAAVMGTIGNGLFRQIVEAKNTTGSAVEIQSSLSTFKHAGADFTSIE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCEEEE
VSSHGLAQHRVEALHFKAAIFTNLTRDHLDYHQSMENYAAAKKRLFTELDTQIKVINADD
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
EIGYQWLTELPDAIAVSMNADFKVGSHQWMKAINIHYHFKGADITFESSWGNGVLHSPLI
HHHHHHHHHCCCCEEEEECCCEEECCHHHHEEEEEEEEEECCEEEEECCCCCCCCCCCCC
GAFNVSNLLLVMTTLLSFGYPLENLLATAKSLKGVCGRMEMIQYPNKPTVIVDYAHTPDA
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
LEKALIAAREHCQGELWCIFGCGGDRDRGKRPLMAQIAEQFAEKIIVTKDNPRTEPQSQI
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCCHHHH
EADIVAGFKNMEKVGIIPDRAQAIQFAIESAVENDVILIAGKGHEHYQIIGSEVVHFSDQ
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCHHEECCCC
EIALDFLK
HHHHEECC
>Mature Secondary Structure
MKKLTALFNLPELKNDIELHNMVLDSRKVKAGDLFVAIKGHQVDGNQFIDSALHSGASAV
CCCHHHHHCCCCHHHHHHHHHHHHCCCCEECCCEEEEEECCCCCCHHHHHHHHHCCHHHH
VSETELSSEHLTVEFIGNVPVVKYHQLARHLSSLADVFYDSPSKNLTLVGVTGTNGKTTI
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHH
SQLLAQWAELLGHRAAVMGTIGNGLFRQIVEAKNTTGSAVEIQSSLSTFKHAGADFTSIE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCEEEE
VSSHGLAQHRVEALHFKAAIFTNLTRDHLDYHQSMENYAAAKKRLFTELDTQIKVINADD
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
EIGYQWLTELPDAIAVSMNADFKVGSHQWMKAINIHYHFKGADITFESSWGNGVLHSPLI
HHHHHHHHHCCCCEEEEECCCEEECCHHHHEEEEEEEEEECCEEEEECCCCCCCCCCCCC
GAFNVSNLLLVMTTLLSFGYPLENLLATAKSLKGVCGRMEMIQYPNKPTVIVDYAHTPDA
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
LEKALIAAREHCQGELWCIFGCGGDRDRGKRPLMAQIAEQFAEKIIVTKDNPRTEPQSQI
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCCHHHH
EADIVAGFKNMEKVGIIPDRAQAIQFAIESAVENDVILIAGKGHEHYQIIGSEVVHFSDQ
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCHHEECCCC
EIALDFLK
HHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA