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The map label for this gene is rfaD [H]

Identifier: 148828275

GI number: 148828275

Start: 1709576

End: 1710502

Strand: Reverse

Name: rfaD [H]

Synonym: CGSHiGG_09225

Alternate gene names: 148828275

Gene position: 1710502-1709576 (Counterclockwise)

Preceding gene: 148828276

Following gene: 148828272

Centisome position: 90.64

GC content: 40.45

Gene sequence:

>927_bases
ATGATTATCGTAACAGGCGGCGCTGGTTTTATTGGCAGCAATATTGTCAAAGCATTGAATGATTTAGGACGCAAAGATAT
TTTAGTGGTCGATAACTTAAAAGATGGCACGAAATTTGCAAATTTAGTTGATTTAGACATCGCAGATTATTGCGATAAAG
AAGATTTCATTGCTTCTATTATTGCGGGCGATGAATTTGGCGATATTGATGCGGTATTCCACGAGGGGGCATGTTCTGCG
ACTACGGAATGGGATGGCAAATACATTATGCACAACAACTACGAATATTCTAAAGAGTTGTTGCATTATTGCCTTGACCG
CGAAATTCCTTTTTTCTATGCCTCAAGTGCAGCAACTTATGGAGATACCAAAGTATTCCGTGAAGAACGTGAATTTGAAG
GTCCATTAAATGTGTATGGCTATTCTAAATTCTTGTTCGATCAATATGTGCGTAACATTTTGCCAGAAGCAAAATCGCCA
GTATGTGGTTTCCGTTATTTCAATGTTTACGGGCCGCGTGAAAATCATAAAGGTTCAATGGCGAGTGTGGCATTCCACTT
GAATAACCAAATCTTAAAAGGCGAAAATCCAAAATTATTTGCAGGCAGCGAAGGGTTCCGCCGTGATTTTGTTTATGTGG
GTGATGTGGCTGCAGTGAATATTTGGTGTTGGCAAAATGGTATTTCGGGTATTTACAACCTTGGTACAGGAAATGCAGAA
AGTTTCCGTGCGGTGGCTGATGCGGTCGTGAAATTCCACGGAAAAGGCGAGATTGAAACTATTCCATTCCCAGAGCATTT
GAAATCTCGTTATCAAGAATATACGCAAGCAGATTTGACTAAACTTCGCTCAACAGGCTACGATAAACCATTCAAAACAG
TCGCGGAAGGCGTGGCTGAATATATGGCGTGGTTGAATAGAAAATAA

Upstream 100 bases:

>100_bases
TATTGGGGATTAAAAGTGCGGTTGTTTTTCGCAGAGTTTTTTGGCTAAGCGGTAGAAAATCTATTACAATACGCAGACTT
ATTTTAAAGAGGACAGAATT

Downstream 100 bases:

>100_bases
ATTATTAATGTAGTAAATCCCCTATAGACTCTCGGTATAGGGGATTTTATTTAGTATAATTGTGATAAATGTTTTAAATA
ACGCTCGTAAGCACTTTGAT

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGACSA
TTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSP
VCGFRYFNVYGPRENHKGSMASVAFHLNNQILKGENPKLFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAEYMAWLNRK

Sequences:

>Translated_308_residues
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGACSA
TTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSP
VCGFRYFNVYGPRENHKGSMASVAFHLNNQILKGENPKLFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAEYMAWLNRK
>Mature_308_residues
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGACSA
TTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSP
VCGFRYFNVYGPRENHKGSMASVAFHLNNQILKGENPKLFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAEYMAWLNRK

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=308, Percent_Identity=77.9220779220779, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71982035, Length=322, Percent_Identity=27.639751552795, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=324, Percent_Identity=27.4691358024691, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 34671; Mature: 34671

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASI
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEEECCHHHHCCHHHHHHHH
IAGDEFGDIDAVFHEGACSATTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATY
HCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCC
GDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSPVCGFRYFNVYGPRENHKGSM
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCC
ASVAFHLNNQILKGENPKLFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
EEEEEEECCEEECCCCCCEEECCCCCCCCEEEECCEEEEEEEEECCCCCCEEECCCCCHH
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAE
HHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YMAWLNRK
HHHHHCCC
>Mature Secondary Structure
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASI
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEEECCHHHHCCHHHHHHHH
IAGDEFGDIDAVFHEGACSATTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATY
HCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCC
GDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSPVCGFRYFNVYGPRENHKGSM
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCC
ASVAFHLNNQILKGENPKLFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
EEEEEEECCEEECCCCCCEEECCCCCCCCEEEECCEEEEEEEEECCCCCCEEECCCCCHH
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAE
HHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YMAWLNRK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA