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The map label for this gene is pyrG [H]

Identifier: 148828238

GI number: 148828238

Start: 1671849

End: 1673486

Strand: Direct

Name: pyrG [H]

Synonym: CGSHiGG_09020

Alternate gene names: 148828238

Gene position: 1671849-1673486 (Clockwise)

Preceding gene: 148828227

Following gene: 148828251

Centisome position: 88.59

GC content: 40.05

Gene sequence:

>1638_bases
ATGGCTACAAATTATATTTTCGTCACTGGCGGTGTGGTTTCATCGTTAGGTAAAGGCATTGCGGCAGCATCATTGGCGGC
AATTTTAGAAGCACGTGGTTTAAACGTAACCATTATGAAGTTAGATCCATATATTAATGTGGATCCTGGTACGATGAGCC
CAACCCAACACGGGGAAGTTTTCGTTACTCAAGATGGCGCAGAAACAGATTTAGATTTAGGTCATTATGAGCGTTTCATC
CGCACGAAAATGACCAAACGTAATAACTTCACTACAGGTAAAATTTATTCTGAAGTATTACGTAAAGAGCGTCGTGGCGA
TTATCTTGGTGCAACAATTCAAGTGATTCCACATATCACTAATGAAATTAAAGATCGCGTTATTGCTGGTGCTCAAGGTC
ACGACGTTGTGATTGTTGAAGTGGGCGGCACTGTTGGGGATATTGAATCACTTCCATTCTTAGAAGCATTACGTCAATTA
GCCGTTCAAGTTGGTCGTGAACATACGTTATTTATGCATTTAACCTTGGTGCCTTACATTCCAACGGCGGGAGAGGTAAA
AACTAAACCAACCCAGCATTCGGTAAAAGAATTGCTTTCTATTGGTATTCAACCGGATGTGTTGATTTGCCGTTCAGATC
GTATGATTCCACCAAATGAACGTGCGAAAATTGCTTTATTTTGTAATGTTGCAGAACGTGCAGTCATTTCATTAAAAGAT
GTGAATTCAATTTATCAAATCCCTGCATTATTAAAATCTCAAGGTTTAGATGATTTTGTCTGTGAACGTTTCCGTTTAAC
TTGCCCTGAAGCAGATCTTACTGAATGGGAACAAGTGCTTTACAAGCAAGCCAATCCAGTGGGAGAAGTAACCATCGGTA
TGGTAGGTAAATACACTGAGTTACCCGATGCCTATAAATCAGTAAATGAAGCCTTAAAACACGCAGGTTTAACGAACCGT
TTAAGTGTAAACATTAAATATATTGATTCCCAAGATGTTGAAACCAAAGGTGTTGAAGTATTAAAAGGCGTTGATGGCAT
TCTTGTTCCAGGGGGATTTGGTTATCGTGGTGTAGAGGGTAAAATTCGTACCGCACAATATGCACGAGAAAACAAAATTC
CTTACTTGGGTATTTGCTTAGGTATGCAGATCGCATTGATTGAATATGCACGTAATGTAGCGGGTTTAACTAAAGCCAAT
TCATCTGAATTTGATAAAGATTGTGAACAACCTGTGGTGGCATTAATCACAGAATGGCAAGATGCGGAGGGTAATACTGA
AGTGCGTACCGATGAATCAGATCTTGGCGGAACAATGCGTTTAGGTGCACAACAATGTCATTTGGTATCAGGTAGCCGCG
CTCGCGAGCTTTATGGCAAAGAAACCATTGAAGAGCGTCATCGTCATCGCTATGAAGTGAACAATACCTTGCTTCCACAA
ATTGAAAAAGCAGGATTGAAAGTAACTGGATTATCTGCAGATAAAAAATTGGTAGAAATTATCGAAGTGCCAAATCATCC
TTGGTTTGTCGCTTGCCAATTCCATCCAGAATTTACTTCTACGCCTCGTGATGGTCATCCGTTGTTTGCAGGCTTTGTAA
AAGCAGCCTATGAAAATCACAAAAAATCTGTGAAGTAA

Upstream 100 bases:

>100_bases
ATAGCTAATTATCTTCGATTTTTGACCGCACTTTTGGTTGATTTTGTCTGTAAGATGATTAGCTATAATCATTTATATTC
CAATACAGATTAGGTTCCCT

Downstream 100 bases:

>100_bases
TTCTTGGTTCTGCAATAAGTGCGGTAAAAAAATGAAATATTTTTATTTGGTTATGATATAACTTGATGTTTTTGAAGTAA
AAAAATCCCCGTTAAATTTA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MATNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTQDGAETDLDLGHYERFI
RTKMTKRNNFTTGKIYSEVLRKERRGDYLGATIQVIPHITNEIKDRVIAGAQGHDVVIVEVGGTVGDIESLPFLEALRQL
AVQVGREHTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRMIPPNERAKIALFCNVAERAVISLKD
VNSIYQIPALLKSQGLDDFVCERFRLTCPEADLTEWEQVLYKQANPVGEVTIGMVGKYTELPDAYKSVNEALKHAGLTNR
LSVNIKYIDSQDVETKGVEVLKGVDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTKAN
SSEFDKDCEQPVVALITEWQDAEGNTEVRTDESDLGGTMRLGAQQCHLVSGSRARELYGKETIEERHRHRYEVNNTLLPQ
IEKAGLKVTGLSADKKLVEIIEVPNHPWFVACQFHPEFTSTPRDGHPLFAGFVKAAYENHKKSVK

Sequences:

>Translated_545_residues
MATNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTQDGAETDLDLGHYERFI
RTKMTKRNNFTTGKIYSEVLRKERRGDYLGATIQVIPHITNEIKDRVIAGAQGHDVVIVEVGGTVGDIESLPFLEALRQL
AVQVGREHTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRMIPPNERAKIALFCNVAERAVISLKD
VNSIYQIPALLKSQGLDDFVCERFRLTCPEADLTEWEQVLYKQANPVGEVTIGMVGKYTELPDAYKSVNEALKHAGLTNR
LSVNIKYIDSQDVETKGVEVLKGVDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTKAN
SSEFDKDCEQPVVALITEWQDAEGNTEVRTDESDLGGTMRLGAQQCHLVSGSRARELYGKETIEERHRHRYEVNNTLLPQ
IEKAGLKVTGLSADKKLVEIIEVPNHPWFVACQFHPEFTSTPRDGHPLFAGFVKAAYENHKKSVK
>Mature_544_residues
ATNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTQDGAETDLDLGHYERFIR
TKMTKRNNFTTGKIYSEVLRKERRGDYLGATIQVIPHITNEIKDRVIAGAQGHDVVIVEVGGTVGDIESLPFLEALRQLA
VQVGREHTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRMIPPNERAKIALFCNVAERAVISLKDV
NSIYQIPALLKSQGLDDFVCERFRLTCPEADLTEWEQVLYKQANPVGEVTIGMVGKYTELPDAYKSVNEALKHAGLTNRL
SVNIKYIDSQDVETKGVEVLKGVDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTKANS
SEFDKDCEQPVVALITEWQDAEGNTEVRTDESDLGGTMRLGAQQCHLVSGSRARELYGKETIEERHRHRYEVNNTLLPQI
EKAGLKVTGLSADKKLVEIIEVPNHPWFVACQFHPEFTSTPRDGHPLFAGFVKAAYENHKKSVK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=45.0628366247756, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=44.7227191413238, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=44.7227191413238, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=44.7227191413238, Blast_Score=474, Evalue=1e-134,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=80.9174311926605, Blast_Score=915, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=606, Percent_Identity=39.1089108910891, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=43.4094903339192, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=42.530755711775, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24664469, Length=558, Percent_Identity=44.6236559139785, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI21357815, Length=504, Percent_Identity=43.2539682539683, Blast_Score=403, Evalue=1e-112,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60240; Mature: 60109

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTQDGAETDLDLGHYERFIRTKMTKRNNFTTGKIYSEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NEIKDRVIAGAQGHDVVIVEVGGTVGDIESLPFLEALRQLAVQVGREHTLFMHLTLVPYI
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCC
PTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRMIPPNERAKIALFCNVAERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHEEEHHH
VNSIYQIPALLKSQGLDDFVCERFRLTCPEADLTEWEQVLYKQANPVGEVTIGMVGKYTE
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCC
LPDAYKSVNEALKHAGLTNRLSVNIKYIDSQDVETKGVEVLKGVDGILVPGGFGYRGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCCC
KIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTKANSSEFDKDCEQPVVALITEWQ
CCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
DAEGNTEVRTDESDLGGTMRLGAQQCHLVSGSRARELYGKETIEERHRHRYEVNNTLLPQ
CCCCCCEEECCHHHCCCEEECCCHHHEEECCCHHHHHHHHHHHHHHHHHHECCCCCCCCC
IEKAGLKVTGLSADKKLVEIIEVPNHPWFVACQFHPEFTSTPRDGHPLFAGFVKAAYENH
HHHCCEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
KKSVK
HCCCC
>Mature Secondary Structure 
ATNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTQDGAETDLDLGHYERFIRTKMTKRNNFTTGKIYSEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NEIKDRVIAGAQGHDVVIVEVGGTVGDIESLPFLEALRQLAVQVGREHTLFMHLTLVPYI
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCC
PTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRMIPPNERAKIALFCNVAERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHEEEHHH
VNSIYQIPALLKSQGLDDFVCERFRLTCPEADLTEWEQVLYKQANPVGEVTIGMVGKYTE
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCC
LPDAYKSVNEALKHAGLTNRLSVNIKYIDSQDVETKGVEVLKGVDGILVPGGFGYRGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCCC
KIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTKANSSEFDKDCEQPVVALITEWQ
CCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
DAEGNTEVRTDESDLGGTMRLGAQQCHLVSGSRARELYGKETIEERHRHRYEVNNTLLPQ
CCCCCCEEECCHHHCCCEEECCCHHHEEECCCHHHHHHHHHHHHHHHHHHECCCCCCCCC
IEKAGLKVTGLSADKKLVEIIEVPNHPWFVACQFHPEFTSTPRDGHPLFAGFVKAAYENH
HHHCCEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
KKSVK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA