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The map label for this gene is nrfA [H]

Identifier: 148828230

GI number: 148828230

Start: 1661701

End: 1663227

Strand: Reverse

Name: nrfA [H]

Synonym: CGSHiGG_08980

Alternate gene names: 148828230

Gene position: 1663227-1661701 (Counterclockwise)

Preceding gene: 148828231

Following gene: 148828229

Centisome position: 88.13

GC content: 40.73

Gene sequence:

>1527_bases
GTGAACGCATTCAAAAAAAGCCTTATTGTGGCAGCGTCTTTCGCGAGTTTAAGCTTATTTAACTCAGCCACTGCTGAGCT
TGTATATAAGCCATTAGAACAACCTGTTGAACCCGCAAAACCTGATTTGAAAATTGAATCTGTAAATGAAAAATTTGCAG
AAAAATATCCAAATCAATATAACAGCTGGCGTTCTACAGCAAACGGCGATGGCGAAAACATTATTTATGCTGATGAAGAA
AATCCGCGCCTAATCGTACTTTGGGGTGGTTATGCATTTGCAAAAGAATATAACGCACCACGCGGTCACTTTTATGCGGT
AACAGATGTGCGTAATATTTTACGTACAGGTGCACCAAAAACTGCAAATGATGGCCCTCAAGCTATGGCATGTTGGACAT
GTAAAGGTCCTGATGTTCCACGTTTAATCGCGGAATGGGGTGAGAAAGATTATTTCAATGCGAAATGGGCAAAGGGAGGG
CCTGAAATCGTGAACTCAATTGGCTGTGCTGACTGTCACGACACAACCTCTAAAGATTTTGCTGAAGGCAAACCTGCATT
ACGTATTGCTCGCCCACACGTATTACGCGCCCTTGATGCTTTAGAAAAAGCCACTGCTGAAAAAGATAAAGCAGAAGGTC
GCCCACACAACAATTTAAGTTTCAACACGGCGGCGCGTACTGAAAAACGTGCAGAAATTTGCGCAAACTGCCATGTTGAA
TACTATTTTGCAGGGGACATCAAGCAAGTAACTTTCCCTTGGGATAATGGTCAAACCGTAGATGATATTGAAAAATATTA
TGATGATATTGGTTTCACTGACTGGACTCACTCACTTTCTAAAGCACCAATGTTAAAAGCACAACACCCTGACTTTGAAA
TTTGGTCTTTAGGTATGCACGGTAAAAATGGGGTAACTTGTGTAGATTGCCATATGCCAAAAGTACAAGGTGCGGACGGT
AAAGTTTACACCGATCACCAAATTCAAAATCCATTTGAGGCATTTGACAGCACTTGTGCAAATTGTCACGATCAAAGCAA
AGAAAAATTACGCGATATTGTTACTTCACGTAAAAAAGAAGTGAAAGATGTAATGGGTCGTTTAGAAGACCAAGTTGTCA
AAGCTCACTTTGAAGCAAAAGAGGCTTGGGACGCAGGTGCAACTAAAAAAGAAATGGAAGCAGCATTAATGGATATTCGT
CATGCTCAATGGCGTTGGGATTACACTGCTGCAAGCCATGGTGGTCATATGCATGCGCCTGAAGTAGTACTTCGTGTATT
AGCTTCTGGCTTAGATAAAGTAGCAGACGCACGTACTAAACTTGCTGTAATCTTAACTAAACACGGTGTGAAAACTCCAG
TTCAAATCCCAGATATTTCAACTGCTGATAAAGCGTGGAAAGTAATGGGTATTGATATTGAAAAAGAACGTAAAGCGAAA
GAAGAATTCTTAAAAACTGTGGTACCACAATGGGAACAACAAGCTAGAGAAAAAGGCTTATTAGTTGATCCACCAGCACA
AAAATAA

Upstream 100 bases:

>100_bases
TTACGCTTGAATGAAAATATATCTACGTTTTGTATGGATATTAATAATAATTCTCAACTTTCTACTTAACCTTTTTATTA
CAACAAATGGAGTAATTATC

Downstream 100 bases:

>100_bases
ATGAAAAATGCACCGCACTTTTTAGTGCAAACAGGAAAGTGCGGTGATTTTCAAGGTCAAATTTGAGGTAACCCAAATGA
ATTTAACTTCTTTAATCAAC

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQYNSWRSTANGDGENIIYADEE
NPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGG
PEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGMHGKNGVTCVDCHMPKVQGADG
KVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLEDQVVKAHFEAKEAWDAGATKKEMEAALMDIR
HAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKHGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
EEFLKTVVPQWEQQAREKGLLVDPPAQK

Sequences:

>Translated_508_residues
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQYNSWRSTANGDGENIIYADEE
NPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGG
PEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGMHGKNGVTCVDCHMPKVQGADG
KVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLEDQVVKAHFEAKEAWDAGATKKEMEAALMDIR
HAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKHGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
EEFLKTVVPQWEQQAREKGLLVDPPAQK
>Mature_508_residues
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQYNSWRSTANGDGENIIYADEE
NPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGG
PEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGMHGKNGVTCVDCHMPKVQGADG
KVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLEDQVVKAHFEAKEAWDAGATKKEMEAALMDIR
HAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKHGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
EEFLKTVVPQWEQQAREKGLLVDPPAQK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=469, Percent_Identity=55.4371002132196, Blast_Score=564, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 56842; Mature: 56842

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCHH
NSWRSTANGDGENIIYADEENPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPK
HHHHCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHCCCCCCCEEEHHHHHHHHHCCCCC
TANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGGPEIVNSIGCADCHDTTSKDF
CCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHH
AEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
HCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGMH
EEEECCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCC
GKNGVTCVDCHMPKVQGADGKVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKE
CCCCCEEEECCCCCCCCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKDVMGRLEDQVVKAHFEAKEAWDAGATKKEMEAALMDIRHAQWRWDYTAASHGGHMHAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHH
EVVLRVLASGLDKVADARTKLAVILTKHGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
EEFLKTVVPQWEQQAREKGLLVDPPAQK
HHHHHHHCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCHH
NSWRSTANGDGENIIYADEENPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPK
HHHHCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHCCCCCCCEEEHHHHHHHHHCCCCC
TANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGGPEIVNSIGCADCHDTTSKDF
CCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHH
AEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
HCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGMH
EEEECCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCC
GKNGVTCVDCHMPKVQGADGKVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKE
CCCCCEEEECCCCCCCCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKDVMGRLEDQVVKAHFEAKEAWDAGATKKEMEAALMDIRHAQWRWDYTAASHGGHMHAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHH
EVVLRVLASGLDKVADARTKLAVILTKHGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
EEFLKTVVPQWEQQAREKGLLVDPPAQK
HHHHHHHCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA