Click here to switch to the map view.

The map label for this gene is pyrH [H]

Identifier: 148828228

GI number: 148828228

Start: 1660300

End: 1660977

Strand: Reverse

Name: pyrH [H]

Synonym: CGSHiGG_08970

Alternate gene names: 148828228

Gene position: 1660977-1660300 (Counterclockwise)

Preceding gene: 148828229

Following gene: 148828226

Centisome position: 88.01

GC content: 41.89

Gene sequence:

>678_bases
ATGACAGTCTGCTCACGCCGAAACTTTGTTTCTGGCATGGGGGCTGTGATCCTTATGACGGGGACATCTTTACCTGCTTT
TGCAAAAGAAAACAAGGCAGATAAACCTAAACGTTACGCAATGATACATGATGAAAATGCGTGTATCGGTTGTACCGCCT
GTATGGACGCATGTCGTGATGTAAACCAAGTGCCAGAAGGCGTTTCTCGCTTAGAGATTCTACGTAGTGAACCTTATGGG
GAATTTCCAAATCAAGAATACGAGTTCTTTCGTCAATCTTGCCAACATTGTACAAATGCACCTTGTGTAGCAGTTTGTCC
AACTGGTGCATCTTTTATTGATAAAGAGACTGGTATTGTAGATGTACATAAAGATTTATGCGTAGGCTGCCAATACTGTA
TAGCAGTTTGCCCATATCGTGTACGTTTTATTCATCCAGTACATCGTACCGCAGATAAATGTAACTTCTGCCGCGATACA
AATTTAGCCAATGGTAAACAACCTGCTTGCGTAGAGGCTTGCCCAACCAAAGCATTAACCTTTGGGGATATGAATGATCC
AACAAGTGCAGTTTCACGTAAAGTGAAAGAAAAACCGGTTTATCGCACTAAAGTGGAATTAGGTACACAACCAAACTTAT
ATCACATTCCATTCCAACATGGGGAGCCTAGAAGATGA

Upstream 100 bases:

>100_bases
TTCAACCAAAACAACGCGTTAAACTTTGTGTAGATTGTCATGGCGAACAACAAAAACGTAAAGCTGAACAAGATAAACTA
ATCGAACAAAAGGATAAACT

Downstream 100 bases:

>100_bases
CGTTAGATTATCCAGTTCCATTTCATACGCCTAATCTAGTGTGGGATTATACCATTGCGATCTACTTGTTTTTATTGGGG
ATCTCATCTGGTGCAGTACA

Product: uridylate kinase

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDACRDVNQVPEGVSRLEILRSEPYG
EFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIVDVHKDLCVGCQYCIAVCPYRVRFIHPVHRTADKCNFCRDT
NLANGKQPACVEACPTKALTFGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR

Sequences:

>Translated_225_residues
MTVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDACRDVNQVPEGVSRLEILRSEPYG
EFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIVDVHKDLCVGCQYCIAVCPYRVRFIHPVHRTADKCNFCRDT
NLANGKQPACVEACPTKALTFGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR
>Mature_224_residues
TVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDACRDVNQVPEGVSRLEILRSEPYGE
FPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIVDVHKDLCVGCQYCIAVCPYRVRFIHPVHRTADKCNFCRDTN
LANGKQPACVEACPTKALTFGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=218, Percent_Identity=65.5963302752293, Blast_Score=310, Evalue=6e-86,
Organism=Escherichia coli, GI226510944, Length=216, Percent_Identity=46.7592592592593, Blast_Score=196, Evalue=8e-52,
Organism=Escherichia coli, GI1789370, Length=222, Percent_Identity=31.5315315315315, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1790326, Length=179, Percent_Identity=30.1675977653631, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1787749, Length=215, Percent_Identity=27.906976744186, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1787122, Length=193, Percent_Identity=31.6062176165803, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787872, Length=193, Percent_Identity=31.6062176165803, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1787740, Length=145, Percent_Identity=29.6551724137931, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787478, Length=137, Percent_Identity=30.6569343065693, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789079, Length=188, Percent_Identity=28.7234042553192, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789067, Length=160, Percent_Identity=31.875, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI87082180, Length=166, Percent_Identity=31.3253012048193, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367245, Length=173, Percent_Identity=30.635838150289, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788811, Length=168, Percent_Identity=31.547619047619, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082179, Length=92, Percent_Identity=35.8695652173913, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082114, Length=190, Percent_Identity=27.3684210526316, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25061; Mature: 24930

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
10.2 %Cys+Met (Translated Protein)
7.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
9.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDACRD
CCCCCCCCHHHCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECCCCEEHHHHHHHHHHH
VNQVPEGVSRLEILRSEPYGEFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCEE
DVHKDLCVGCQYCIAVCPYRVRFIHPVHRTADKCNFCRDTNLANGKQPACVEACPTKALT
ECHHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEE
FGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDACRD
CCCCCCCHHHCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECCCCEEHHHHHHHHHHH
VNQVPEGVSRLEILRSEPYGEFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCEE
DVHKDLCVGCQYCIAVCPYRVRFIHPVHRTADKCNFCRDTNLANGKQPACVEACPTKALT
ECHHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEE
FGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]