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The map label for this gene is dlgD

Identifier: 148828197

GI number: 148828197

Start: 1625129

End: 1626127

Strand: Reverse

Name: dlgD

Synonym: CGSHiGG_08775

Alternate gene names: 148828197

Gene position: 1626127-1625129 (Counterclockwise)

Preceding gene: 148828203

Following gene: 148828196

Centisome position: 86.17

GC content: 38.94

Gene sequence:

>999_bases
ATGAGAGTTTCTTATGATGAATTAAAAAATGAGTTTAAGCGTGTTTTATTAGATCGTCAGCTTACTGAGGAATTAGCAGA
AGAATGTGCTACTGCATTTGCAGATACAACACAAGCTGGTGCTTATTCACATGGTGTAAATCGTTTTCCTCGTTTTATTC
AACAACTTGAACAGGGAGATATTGTACCCAATGCAATCCCTACAAAAGTATTAAGTTTAGGTTCTATAGAACAGTGGGAT
GCACATCAAGCAATAGGTAATTTGACTGCCAAAAAAATGATGGATAGAGCAATAGAGTTAGCTTCACAACATGGCGTCGG
TGTAATAGCGCTTCGTAATGCTAATCACTGGATGCGAGGAGGATCTTACGGTTGGCAAGCTGCTGAAAAAGGTTATATCG
GGATCTGTTGGACAAATGCATTAGCGGTAATGCCCCCTTGGGGAGCTAAAGAATGCCGTATAGGGACGAATCCATTAATT
ATTGCAGTTCCTACCACACCAATCACTATGGTGGATATGTCTTGCTCAATGTATTCTTATGGAATGTTAGAAGTCCATCG
TTTAGCAGGTCGTCAAACCTTTGTTGATGCAGGTTTTGATGATGAAGGAAATTTAACTCGTGATCCCTCTATTGTAGAAA
AAAACCGTCGTTTGCTCCCTATGGGATTTTGGAAAGGTTCTGGTTTATCTATCGTGCTGGATATGATTGCAACCTTACTA
TCGAATGGTGAATCGACAGTTGCTGTAACTGAAGATAAAAACGATGAATACTGTGTTTCTCAAGTGTTTATCGCCATTGA
AGTAGATCGCTTAATTGATGGTAAAAGCAAAGATGAAAAATTAAACCGCATTATGGATTATGTAAAAACAGCAGAACGAT
CTGATCCAACTCAATCTGTTCGCCTGCCTGGCCATGAATTTACCACTATTCTTTCTGATAACCAAGCTAATGGTATTCCA
GTGGATGAACGAGTTTGGGCAAAATTAAAGACGTTATAA

Upstream 100 bases:

>100_bases
TTTATAAATAATTTATTTATTTTACATTTATTGACTTAGCTTTCTTATTTTTGTAAATTATGAAACATGATTTTACCATA
TGAAATTAAGAGAGAGAAAA

Downstream 100 bases:

>100_bases
CAGCAATATGTTTCCAGTAAAACGCCATAATATAAAACGAATCACATTTGTGATAATCAATTGGAGGTGTAATGATGAGA
TCTCTCGCCCACTTTATTAA

Product: 2,3-diketo-L-gulonate reductase

Products: NA

Alternate protein names: 2,3-DKG reductase; 3-dehydro-L-gulonate 2-dehydrogenase

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRVSYDELKNEFKRVLLDRQLTEELAEECATAFADTTQAGAYSHGVNRFPRFIQQLEQGDIVPNAIPTKVLSLGSIEQWD
AHQAIGNLTAKKMMDRAIELASQHGVGVIALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
IAVPTTPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDEGNLTRDPSIVEKNRRLLPMGFWKGSGLSIVLDMIATLL
SNGESTVAVTEDKNDEYCVSQVFIAIEVDRLIDGKSKDEKLNRIMDYVKTAERSDPTQSVRLPGHEFTTILSDNQANGIP
VDERVWAKLKTL

Sequences:

>Translated_332_residues
MRVSYDELKNEFKRVLLDRQLTEELAEECATAFADTTQAGAYSHGVNRFPRFIQQLEQGDIVPNAIPTKVLSLGSIEQWD
AHQAIGNLTAKKMMDRAIELASQHGVGVIALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
IAVPTTPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDEGNLTRDPSIVEKNRRLLPMGFWKGSGLSIVLDMIATLL
SNGESTVAVTEDKNDEYCVSQVFIAIEVDRLIDGKSKDEKLNRIMDYVKTAERSDPTQSVRLPGHEFTTILSDNQANGIP
VDERVWAKLKTL
>Mature_332_residues
MRVSYDELKNEFKRVLLDRQLTEELAEECATAFADTTQAGAYSHGVNRFPRFIQQLEQGDIVPNAIPTKVLSLGSIEQWD
AHQAIGNLTAKKMMDRAIELASQHGVGVIALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
IAVPTTPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDEGNLTRDPSIVEKNRRLLPMGFWKGSGLSIVLDMIATLL
SNGESTVAVTEDKNDEYCVSQVFIAIEVDRLIDGKSKDEKLNRIMDYVKTAERSDPTQSVRLPGHEFTTILSDNQANGIP
VDERVWAKLKTL

Specific function: Catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH, to form 3-keto-L-gulonate

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family. DlgD subfamily

Homologues:

Organism=Escherichia coli, GI1790000, Length=332, Percent_Identity=71.6867469879518, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1787020, Length=335, Percent_Identity=24.4776119402985, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786727, Length=336, Percent_Identity=24.1071428571429, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17507179, Length=320, Percent_Identity=25, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17536633, Length=333, Percent_Identity=25.5255255255255, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24667908, Length=325, Percent_Identity=27.0769230769231, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45553211, Length=325, Percent_Identity=27.0769230769231, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24667912, Length=325, Percent_Identity=27.0769230769231, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24667904, Length=325, Percent_Identity=27.0769230769231, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24658010, Length=267, Percent_Identity=25.8426966292135, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648360, Length=306, Percent_Identity=24.1830065359477, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DLGD_HAEIG (A5UIG1)

Other databases:

- EMBL:   CP000672
- RefSeq:   YP_001292950.1
- ProteinModelPortal:   A5UIG1
- SMR:   A5UIG1
- STRING:   A5UIG1
- GeneID:   5227614
- GenomeReviews:   CP000672_GR
- KEGG:   hiq:CGSHiGG_08775
- eggNOG:   COG2055
- HOGENOM:   HBG293006
- OMA:   TNALAVM
- ProtClustDB:   PRK13260
- GO:   GO:0005737
- HAMAP:   MF_00820
- InterPro:   IPR003767
- PANTHER:   PTHR11091

Pfam domain/function: PF02615 Ldh_2; SSF89733 MDH_LDH

EC number: =1.1.1.130

Molecular weight: Translated: 36913; Mature: 36913

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: ACT_SITE 44-44

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSYDELKNEFKRVLLDRQLTEELAEECATAFADTTQAGAYSHGVNRFPRFIQQLEQGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHCC
IVPNAIPTKVLSLGSIEQWDAHQAIGNLTAKKMMDRAIELASQHGVGVIALRNANHWMRG
CCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHCC
GSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLIIAVPTTPITMVDMSCSMYSY
CCCCCHHHCCCEEEEEECCCEEEECCCCCCCCEECCCCEEEEECCCCEEEEECCCCHHHH
GMLEVHRLAGRQTFVDAGFDDEGNLTRDPSIVEKNRRLLPMGFWKGSGLSIVLDMIATLL
HHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
SNGESTVAVTEDKNDEYCVSQVFIAIEVDRLIDGKSKDEKLNRIMDYVKTAERSDPTQSV
CCCCCEEEEECCCCCCHHHHHEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEE
RLPGHEFTTILSDNQANGIPVDERVWAKLKTL
ECCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MRVSYDELKNEFKRVLLDRQLTEELAEECATAFADTTQAGAYSHGVNRFPRFIQQLEQGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHCC
IVPNAIPTKVLSLGSIEQWDAHQAIGNLTAKKMMDRAIELASQHGVGVIALRNANHWMRG
CCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHCC
GSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLIIAVPTTPITMVDMSCSMYSY
CCCCCHHHCCCEEEEEECCCEEEECCCCCCCCEECCCCEEEEECCCCEEEEECCCCHHHH
GMLEVHRLAGRQTFVDAGFDDEGNLTRDPSIVEKNRRLLPMGFWKGSGLSIVLDMIATLL
HHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
SNGESTVAVTEDKNDEYCVSQVFIAIEVDRLIDGKSKDEKLNRIMDYVKTAERSDPTQSV
CCCCCEEEEECCCCCCHHHHHEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEE
RLPGHEFTTILSDNQANGIPVDERVWAKLKTL
ECCCCCEEEEEECCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA