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The map label for this gene is dnaN [H]

Identifier: 148828163

GI number: 148828163

Start: 1583192

End: 1584292

Strand: Reverse

Name: dnaN [H]

Synonym: CGSHiGG_08555

Alternate gene names: 148828163

Gene position: 1584292-1583192 (Counterclockwise)

Preceding gene: 148828164

Following gene: 148828160

Centisome position: 83.95

GC content: 38.6

Gene sequence:

>1101_bases
ATGCAATTTAGCATTTCAAGAGAAAATTTATTAAAACCGTTGCAGCAAGTATGTGGCGTATTGAGTAATCGCCCAAACAT
TCCTGTATTAAACAACGTATTGTTGCAAATTGAAGACAATCGTCTGACTATCACTGGTACAGATTTAGAAGTCGAATTAT
CGAGTCAAACTCAGCTTTCATCGTCTTCTGAAAATGGCACTTTTACCATTCCAGCAAAAAAATTCTTAGATATTTGCCGC
ACTTTATCTGATGATTCAGAAATCACGGTGACTTTTGAACAAGATCGTGCTTTAGTGCAGTCTGGGCGTAGCCGTTTTAC
GCTGACAACCCAACCTGCCGAAGAATATCCAAACCTCACCGATTGGCAGTCTGAAGTGGATTTTCAACTACCGCAAAATA
CCTTACGCCGTTTAATTGAATCCACTCAATTTTCTATGGCAAACCAAGATGCACGCTATTTCTTAAATGGTATGAAATTT
GAAACAGAGGGCAATTTATTGCGAACGGTGGCAACAGATGGTCATAGACTTGCGGTGTGTACTATCTCACTTGAACAAGA
ATTACAAAATCATTCTGTGATTTTACCTCGTAAAGGCGTGTTAGAACTTGTTCGTTTATTAGAAACAAACGATGAACCAG
CACGTTTACAAATTGGCACCAACAATTTACGTGTCCATTTAAAAAACACAGTATTTACATCAAAATTAATTGATGGTCGC
TTTCCTGACTATCGCCGAGTATTGCCTCGTAATGCCACAAAAATTGTCGAAGGCAATTGGGAAATGCTGAAACAGGCTTT
TGTTCGTGCGTCTATTTTATCTAATGAACGCGCACGCAGCGTACGTTTAAGCTTAAAGGAAAACCAACTTAAAATTACTG
CCTCAAATACAGAACACGAAGAAGCAGAAGAAATTGTCGATGTCAATTACAATGGCGAAGAATTAGAAGTGGGCTTTAAT
GTGACCTATATTCTTGATGTGCTGAATGCACTTAAATGTAATCAAGTGCGAATGTGCTTAACTGATGCGTTCTCCAGTTG
CTTAATTGAAAACTGTGAAGACAGCAGCTGCGAATACGTCATTATGCCAATGCGTTTATAA

Upstream 100 bases:

>100_bases
CGGAAGGGGGCTGGTTAATCCCCCCTCAGCCTTCGGCAGCTCCCCCCAGAAATGGGTGGAGCCAAGAAATACAATAAATC
CTGCATAAAAGGAGAACGCA

Downstream 100 bases:

>100_bases
TATGGCGATTTCTCGTTTACTGGTCGAAAAGTTTCGTAACTTAACAGCAGTGGATCTTGATTTTGATCCCTGCTTTAACT
TTTTAATCGGCAACAACGGC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLSSSSENGTFTIPAKKFLDICR
TLSDDSEITVTFEQDRALVQSGRSRFTLTTQPAEEYPNLTDWQSEVDFQLPQNTLRRLIESTQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
FPDYRRVLPRNATKIVEGNWEMLKQAFVRASILSNERARSVRLSLKENQLKITASNTEHEEAEEIVDVNYNGEELEVGFN
VTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYVIMPMRL

Sequences:

>Translated_366_residues
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLSSSSENGTFTIPAKKFLDICR
TLSDDSEITVTFEQDRALVQSGRSRFTLTTQPAEEYPNLTDWQSEVDFQLPQNTLRRLIESTQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
FPDYRRVLPRNATKIVEGNWEMLKQAFVRASILSNERARSVRLSLKENQLKITASNTEHEEAEEIVDVNYNGEELEVGFN
VTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYVIMPMRL
>Mature_366_residues
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLSSSSENGTFTIPAKKFLDICR
TLSDDSEITVTFEQDRALVQSGRSRFTLTTQPAEEYPNLTDWQSEVDFQLPQNTLRRLIESTQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
FPDYRRVLPRNATKIVEGNWEMLKQAFVRASILSNERARSVRLSLKENQLKITASNTEHEEAEEIVDVNYNGEELEVGFN
VTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYVIMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=62.0218579234973, Blast_Score=497, Evalue=1e-142,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41656; Mature: 41656

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCEEEEEECCEEEEEECCEEEEECCCCCCC
SSSENGTFTIPAKKFLDICRTLSDDSEITVTFEQDRALVQSGRSRFTLTTQPAEEYPNLT
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEEEECCHHHCCCCC
DWQSEVDFQLPQNTLRRLIESTQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAVC
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEEE
TISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
EEEHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCEEEHHHHCCC
FPDYRRVLPRNATKIVEGNWEMLKQAFVRASILSNERARSVRLSLKENQLKITASNTEHE
CCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEIVDVNYNGEELEVGFNVTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYV
HHHHEEECCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
IMPMRL
EEECCC
>Mature Secondary Structure
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCEEEEEECCEEEEEECCEEEEECCCCCCC
SSSENGTFTIPAKKFLDICRTLSDDSEITVTFEQDRALVQSGRSRFTLTTQPAEEYPNLT
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEEEECCHHHCCCCC
DWQSEVDFQLPQNTLRRLIESTQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAVC
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEEE
TISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
EEEHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCEEEHHHHCCC
FPDYRRVLPRNATKIVEGNWEMLKQAFVRASILSNERARSVRLSLKENQLKITASNTEHE
CCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEIVDVNYNGEELEVGFNVTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYV
HHHHEEECCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
IMPMRL
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]