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The map label for this gene is eno [H]

Identifier: 148828109

GI number: 148828109

Start: 1520954

End: 1522264

Strand: Reverse

Name: eno [H]

Synonym: CGSHiGG_08225

Alternate gene names: 148828109

Gene position: 1522264-1520954 (Counterclockwise)

Preceding gene: 148828110

Following gene: 148828108

Centisome position: 80.66

GC content: 41.5

Gene sequence:

>1311_bases
ATGGCAAAAATCGTTAAAGTGATTGGTCGCGAAATCATCGACTCACGCGGTAATCCAACTGTTGAAGCTGAAGTTCATCT
TGAAGGTGGTTTTGTTGGTTTAGCAGCAGCTCCATCTGGTGCATCAACTGGTTCTCGTGAAGCATTAGAATTACGTGACG
GCGACAAATCTCGTTTCTTAGGTAAAGGCGTATTAAAAGCTGTGGCTGCAGTGAACAACGAAATTGCACAAGCTATCGTT
GGTAAAGATGCAACAAACCAAGCTGAAATCGACCAAATCATGATCGATTTAGACGGAACCGAAAACAAATCTAACTTTGG
TGCAAATGCAATCTTGGCGGTATCTTTAGCAAACGCAAAAGCAGCTGCAGCATCTAAAGGTTTACCACTTTACGCTTACA
TTGCAGAATTAAATGGCACTGCTGGTGTTTATTCTATGCCATTACCAATGATGAACATCATTAACGGTGGCGAACATGCA
GATAACAACGTTGATATCCAAGAATTCATGATTCAACCAGTTGGTGCGAAAACATTACGTGAAGCACTTCGTATCGGTGC
TGAAGTATTCCACAACTTGGCGAAAGTATTAAAAGCTAAAGGCATGAGCACTGCAGTTGGTGACGAAGGTGGTTTCGCAC
CAAACTTAGCCTCTAACGCAGACGCTTTAGCCTGTATCAAAGAAGCAGTAGAAAAAGCAGGTTACGTATTAGGTAAAGAC
GTTACTTTAGCGATGGACTGCGCATCTTCTGAGTTCTATAACAAAGAAAATGGTATGTACGAAATGAAAGGTGAAGGTAA
ATCATTCACTTCTCAAGAATTCACTCACTACTTAGAAGAATTATGCAAAGAGTACCCAATCGTGTCTATCGAAGATGGTC
AAGATGAATCTGACTGGGAAGGTTTTGCATACCAAACTAAAGTGTTAGGCGACCGCGTTCAATTAGTGGGCGATGATTTA
TTCGTAACGAATACCAAAATCTTAAAAGAAGGTATCGAAAAAGGTATCGCAAACTCTATCTTAATCAAATTCAACCAAAT
CGGTTCTTTAACTGAAACTTTAGCAGCAATTAAAATGGCGAAAGATGCAGGTTACACCGCTGTAATCTCTCACCGTTCAG
GCGAAACTGAAGATGCAACTATCGCTGATTTAGCGGTGGGTACAGCAGCAGGTCAAATCAAAACTGGTTCTATGAGCCGT
TCTGACCGTATTGCGAAATACAACCAATTAATCCGTATCGAAGAAGCATTAGAACGCGCAGGTACTCCAGCAGCATTCCC
AGGCTTAAAAGCGGTTAAAGGTCAAGCGTAA

Upstream 100 bases:

>100_bases
ACGCATTTTTTACAAGACAAAATCTCAAAATAGCTTTATCATATCGCCTGTTTTAGGCTCGCCTAACCCGTTTTAATTAA
CTTAATTGAGGAAAATAAAA

Downstream 100 bases:

>100_bases
TTCACGCTGAATTTACTGTAAAATCTCACCGCACTTTGTATAAAAGTGCGGTGTTTTTATATCTAATATTTAAAGGAAAT
AGAATGTTACATTTAACTTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVAAVNNEIAQAIV
GKDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAKAAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLREALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWEGFAYQTKVLGDRVQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRIAKYNQLIRIEEALERAGTPAAFPGLKAVKGQA

Sequences:

>Translated_436_residues
MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVAAVNNEIAQAIV
GKDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAKAAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLREALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWEGFAYQTKVLGDRVQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRIAKYNQLIRIEEALERAGTPAAFPGLKAVKGQA
>Mature_435_residues
AKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVAAVNNEIAQAIVG
KDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAKAAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHAD
NNVDIQEFMIQPVGAKTLREALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKDV
TLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWEGFAYQTKVLGDRVQLVGDDLF
VTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRS
DRIAKYNQLIRIEEALERAGTPAAFPGLKAVKGQA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=52.3041474654378, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=52.3041474654378, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=50.9216589861751, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=51.3824884792627, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=47.4537037037037, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=26.6862170087977, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=26.6862170087977, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=26.6862170087977, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1789141, Length=437, Percent_Identity=84.6681922196796, Blast_Score=726, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17536383, Length=436, Percent_Identity=52.7522935779816, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71995829, Length=425, Percent_Identity=53.4117647058823, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=195, Percent_Identity=45.6410256410256, Blast_Score=168, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=436, Percent_Identity=49.3119266055046, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323985, Length=425, Percent_Identity=48.2352941176471, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324974, Length=425, Percent_Identity=48.2352941176471, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324969, Length=425, Percent_Identity=48.2352941176471, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=436, Percent_Identity=48.394495412844, Blast_Score=346, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24580918, Length=424, Percent_Identity=51.6509433962264, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=424, Percent_Identity=51.6509433962264, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=424, Percent_Identity=51.6509433962264, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=424, Percent_Identity=51.6509433962264, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=418, Percent_Identity=52.1531100478469, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=418, Percent_Identity=52.1531100478469, Blast_Score=381, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46133; Mature: 46002

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFL
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVAAVNNEIAQAIVGKDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCH
AAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLR
HHHHCCCCEEEEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWE
EEEEEECCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
GFAYQTKVLGDRVQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CEEEHHHHHCCHHHEECCCEEEEHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRIAKYNQLIRIEEALERA
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHC
GTPAAFPGLKAVKGQA
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFL
HHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVAAVNNEIAQAIVGKDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCH
AAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLR
HHHHCCCCEEEEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWE
EEEEEECCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
GFAYQTKVLGDRVQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CEEEHHHHHCCHHHEECCCEEEEHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRIAKYNQLIRIEEALERA
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHC
GTPAAFPGLKAVKGQA
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA