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The map label for this gene is glyS [H]

Identifier: 148828101

GI number: 148828101

Start: 1512788

End: 1514854

Strand: Reverse

Name: glyS [H]

Synonym: CGSHiGG_08185

Alternate gene names: 148828101

Gene position: 1514854-1512788 (Counterclockwise)

Preceding gene: 148828102

Following gene: 148828097

Centisome position: 80.27

GC content: 44.7

Gene sequence:

>2067_bases
ATGACAACCCAAAACTTCCTAGTAGAAATCGGCACAGAAGAGCTGCCACCAAAAGCGCTTAAAACATTAGCGACCTCTTT
TGCGGATAACGTTGAGGCGGAATTGAACCAAGCAGGTTTAACCTTCGACAAAATCGAATGGTTTGCGGCGCCACGTCGTT
TGGCGGTGAAAGTGTTGAACTTGGCGAAACAACAACCGAGCAAAGAAATCGAAAAACGAGGGCCTGCAGTGTCAGCAGCC
TTTGATGCGGAAGGTAAACCAACTAAAGCGGCAGAAGGCTGGGCGCGTGGTTGTGGTATTACCGTTGAGCAAGCAGAACG
CATTGCAACTGATAAAGGCGAATGGCTCGTTCACCGTGCAAAAATTGAAGGGCAACCGACCAAAAACTTACTTAACGATA
TTGTGGCAAACGCGTTAGCGAAATTGCCAATTCCAAAACCAATGCGTTGGGCAGATAAAACCGTGCAATTTATTCGCCCA
GTTCACACCGTGACTATGTTGTTAGGCGATGAGTTAATTGAAGGCGAAATTTTAGGTGTGGCAAGTGCTCGCACCATTCG
CGGTCACCGTTTCTTAGGCGAGAAAGAATTTGACATTCAACATGCAGACCAATATCCGCAATTATTGCGTGATAAAGGTT
CTGTGGTAGCAGACTTCAATGAACGTAAAGCAGAAATTTTGGCAAAATCTCAAGCAAAAGCGACCGCACTTGGTGGCGTG
GCTGACATTGAAGAAAGCCTGCTTGAAGAAGTCACTTCCTTGGTGGAATATCCAAATGTTTTAGCGGCAAAATTTGAAGA
ACGTTTCTTAGCGGTGCCTGCGGAAGCCTTGGTTTACACCATGAAAGGAGACCAAAAATATTTCCCGATTTATGATAAAG
ACGGCAGATTATTACCGCACTTTATTTTCGTATCGAACATCAACCCAGAAGATCCAACCGCGATTATCGAAGGGAACGAA
AAAGTGGTTCGTCCACGTTTAACCGATGCGGAATTCTTCTTCAAAACCGACTTAAAACAAAAACTGGTTGATCGTTTACC
ACGTTTAGAAACCGTGTTGTTCCAACAACAATTGGGTACATTGAAAGATAAAACTGATCGCATTGAGCAACTTGCAGGCG
AAATTGCAAAACAAATAGGCACAGACGAAGCGAAAGCAAAACGTGCTGGCTTACTGTCAAAATGTGACTTGATGACCAAC
ATGGTGTTTGAATTCACCGATACGCAAGGCGTAATGGGGATGCACTATGCCCGTCATGACGGTGAAGATGAAGAAGTGGC
GGTGGCGTTAAACGAACAATATATGCCACGCTTTGCGGGTGATGAATTACCTAAGTCTTTAGTGGCAAGTGCGGTCGCTT
TAGCGGATAAATTTGACACCTTAACGGGTATCTTCGGCATCGGCCAAGCACCAAAAGGTAGTGCAGACCCATTTGCATTA
CGTCGTGCGGCATTAGGTGCATTACGTATTATCGTAGAGAAAAACCTACCACTTGATTTAGAAGATTTAGTGAAAAAATC
AGCCGCACTTTTCGGTGATAAACTCACGAATCAAAATGTGGTTGCTGATGTGGTGGACTTCATGCTCGGCCGTTTCCGTG
CATGGTATCAAGATGAAGGCATTGAGGTGGATGTAATTCAAGCGGTATTAGCACGTCGTCCAACTCGCCCTGCTGACTTT
GATGCGCGAGTGCGTGCGGTTTCACATTTCCGTACTTTAGATTCAGCGGAAGCGTTAGCAGCAGCAAATAAACGTGTAAG
TAACATCTTAGCGAAAGCAGATGCTGCAATTGGCGAGATCAATTTAACCGTTTGCGTAGAGCAAGCAGAAAAAGCTCTTG
CTGAAGCGGTGCTTGCATTACGCACTGAAGTACAACCGCTTATCGCACAAGGCGATTACACGACTGTATTGGATAAATTA
GCAAACTTACGTGCGCCAGTAGACAGTTTCTTTGATAACGTGATGGTAAATGCTGAAGATCCAGCACTACGCCAAAACCG
CTTAGCGATTTTAAATACGTTACAAGGGTTATTCTTACAAGTAGCTGATATTTCAGTGCTTCAATAA

Upstream 100 bases:

>100_bases
ATCCCCTCTTTGGCAAAGAGGGGTAGGGGCGATTTGGCAGAAATGATAACGAAGAAAATAAGAATTTAAATCTACAGAAT
TTTTAAATAAGAGAAACAAA

Downstream 100 bases:

>100_bases
TGTAAAAAGGGATGGTTGATTTTTCGTCAATCATCCCTTCCTTTATAGACTTCAAGTGATTATTTCTCTATAATTACCGC
ACTTTCCCCCCTTAGCTCAG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MTTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLTFDKIEWFAAPRRLAVKVLNLAKQQPSKEIEKRGPAVSAA
FDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHRAKIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRP
VHTVTMLLGDELIEGEILGVASARTIRGHRFLGEKEFDIQHADQYPQLLRDKGSVVADFNERKAEILAKSQAKATALGGV
ADIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGRLLPHFIFVSNINPEDPTAIIEGNE
KVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGTLKDKTDRIEQLAGEIAKQIGTDEAKAKRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
RRAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEGIEVDVIQAVLARRPTRPADF
DARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEINLTVCVEQAEKALAEAVLALRTEVQPLIAQGDYTTVLDKL
ANLRAPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISVLQ

Sequences:

>Translated_688_residues
MTTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLTFDKIEWFAAPRRLAVKVLNLAKQQPSKEIEKRGPAVSAA
FDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHRAKIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRP
VHTVTMLLGDELIEGEILGVASARTIRGHRFLGEKEFDIQHADQYPQLLRDKGSVVADFNERKAEILAKSQAKATALGGV
ADIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGRLLPHFIFVSNINPEDPTAIIEGNE
KVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGTLKDKTDRIEQLAGEIAKQIGTDEAKAKRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
RRAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEGIEVDVIQAVLARRPTRPADF
DARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEINLTVCVEQAEKALAEAVLALRTEVQPLIAQGDYTTVLDKL
ANLRAPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISVLQ
>Mature_687_residues
TTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLTFDKIEWFAAPRRLAVKVLNLAKQQPSKEIEKRGPAVSAAF
DAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHRAKIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRPV
HTVTMLLGDELIEGEILGVASARTIRGHRFLGEKEFDIQHADQYPQLLRDKGSVVADFNERKAEILAKSQAKATALGGVA
DIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGRLLPHFIFVSNINPEDPTAIIEGNEK
VVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGTLKDKTDRIEQLAGEIAKQIGTDEAKAKRAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFALR
RAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEGIEVDVIQAVLARRPTRPADFD
ARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEINLTVCVEQAEKALAEAVLALRTEVQPLIAQGDYTTVLDKLA
NLRAPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISVLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=69.2307692307692, Blast_Score=966, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75831; Mature: 75700

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLTFDKIEWFAAPRRLAVKVLN
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LAKQQPSKEIEKRGPAVSAAFDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHRA
HHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
KIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRPVHTVTMLLGDELIEGEILGV
ECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ASARTIRGHRFLGEKEFDIQHADQYPQLLRDKGSVVADFNERKAEILAKSQAKATALGGV
CCHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHCCH
ADIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGRLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPEDPTAIIEGNEKVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGT
EEEEECCCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LKDKTDRIEQLAGEIAKQIGTDEAKAKRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEG
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
IEVDVIQAVLARRPTRPADFDARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEI
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
NLTVCVEQAEKALAEAVLALRTEVQPLIAQGDYTTVLDKLANLRAPVDSFFDNVMVNAED
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCEEECCCC
PALRQNRLAILNTLQGLFLQVADISVLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLTFDKIEWFAAPRRLAVKVLN
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LAKQQPSKEIEKRGPAVSAAFDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHRA
HHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
KIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRPVHTVTMLLGDELIEGEILGV
ECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ASARTIRGHRFLGEKEFDIQHADQYPQLLRDKGSVVADFNERKAEILAKSQAKATALGGV
CCHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHCCH
ADIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGRLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPEDPTAIIEGNEKVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGT
EEEEECCCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LKDKTDRIEQLAGEIAKQIGTDEAKAKRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEG
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
IEVDVIQAVLARRPTRPADFDARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEI
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
NLTVCVEQAEKALAEAVLALRTEVQPLIAQGDYTTVLDKLANLRAPVDSFFDNVMVNAED
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCEEECCCC
PALRQNRLAILNTLQGLFLQVADISVLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA