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The map label for this gene is thyA [H]

Identifier: 148828083

GI number: 148828083

Start: 1490687

End: 1491538

Strand: Direct

Name: thyA [H]

Synonym: CGSHiGG_08085

Alternate gene names: 148828083

Gene position: 1490687-1491538 (Clockwise)

Preceding gene: 148828082

Following gene: 148828084

Centisome position: 78.99

GC content: 40.73

Gene sequence:

>852_bases
ATGAAGCAATATCTTGAGCTTTGTCGCCGCATTGTTAGTGAAGGGGAATGGGTTGCTAATGAACGTACAGGTAAGCGTTG
CCTTACGGTCATTAATGCAGATTTAGAATATGACGTGGCGAATAATCAATTTCCGCTGATTACTACCCGTAAAAGTTATT
GGAAAGCGGCCATTGCTGAATTTCTAGGTTATATTCGTGGATATGACAATGCCGCTGATTTCCGCGAACTTGGCACGAAA
ACTTGGGATGCTAATGCGAATGAAAATGCAGCTTGGCTTGCAAATCCGCATCGTAGAGGCGTTGATGATATGGGGCGCGT
ATATGGTGTGCAAGGCAGAGCATGGCGTAAGCCTAATGGAGAAACCATAGATCAGCTACGTAAAATTGTTAATAACTTAA
CGAAAGGTATTGATGATAGAGGAGAGATTTTAACTTTTTTTAACCCTGGGGAATTTGATCTTGGTTGTCTTCGTCCTTGT
ATGCACACGCATACTTTTTCTCTTGTGGGTGATACTTTACATCTTACTAGCTATCAGCGTTCCTGTGATGTTCCGCTTGG
ATTGAATTTCAATCAAATTCAGGTGTTTACCTTCTTAGCGCTTATGGCTCAGATCACGGGCAAAAAAGCCGGCAAGGCAT
ATCATAAAATTGTGAATGCGCATATTTATGAAGATCAGCTTGAGTTAATGCGTGACGTACAACTTAAACGCGAGCCTTTC
CCATTGCCAAAATTAGAAATAAATCCAGATATAAAAACCCTTGAAGATTTAGAAACTTGGGTCACGATGGATGATTTTAA
AGTCGTTGGCTATCAATCTCATGAGCCGATTAAATATCCTTTTTCCGTCTAA

Upstream 100 bases:

>100_bases
ACGAGGGCAAGCACTTTGTTTACCGATGATTATTGGTGGTGCTTTCATTATGGCTTGGGCTTATTCACGCAAAAGTGCGG
TCATAAAATAGGAGATTTTT

Downstream 100 bases:

>100_bases
TGGATGCAGCAAAAGTGCGGTCAGAATTTGACGAGAAAATGATGCGCTACGCCCTTGAGCTTGCCGATAAAGCGGAAGCG
TTAGGTGAGATTCCTGTGGG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKQYLELCRRIVSEGEWVANERTGKRCLTVINADLEYDVANNQFPLITTRKSYWKAAIAEFLGYIRGYDNAADFRELGTK
TWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNGETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPC
MHTHTFSLVGDTLHLTSYQRSCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV

Sequences:

>Translated_283_residues
MKQYLELCRRIVSEGEWVANERTGKRCLTVINADLEYDVANNQFPLITTRKSYWKAAIAEFLGYIRGYDNAADFRELGTK
TWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNGETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPC
MHTHTFSLVGDTLHLTSYQRSCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV
>Mature_283_residues
MKQYLELCRRIVSEGEWVANERTGKRCLTVINADLEYDVANNQFPLITTRKSYWKAAIAEFLGYIRGYDNAADFRELGTK
TWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNGETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPC
MHTHTFSLVGDTLHLTSYQRSCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=32.8719723183391, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=34.2756183745583, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71993377, Length=276, Percent_Identity=28.6231884057971, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324648, Length=302, Percent_Identity=28.1456953642384, Blast_Score=112, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17137556, Length=291, Percent_Identity=29.8969072164948, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32537; Mature: 32537

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLELCRRIVSEGEWVANERTGKRCLTVINADLEYDVANNQFPLITTRKSYWKAAIAE
CHHHHHHHHHHHCCCCEEECCCCCHHEEEEEECCEEEEECCCCCCEEECCHHHHHHHHHH
FLGYIRGYDNAADFRELGTKTWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCC
ETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPCMHTHTFSLVGDTLHLTSYQR
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHH
SCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
CCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV
CCCCEEECCCHHHHHHHHHHEECCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKQYLELCRRIVSEGEWVANERTGKRCLTVINADLEYDVANNQFPLITTRKSYWKAAIAE
CHHHHHHHHHHHCCCCEEECCCCCHHEEEEEECCEEEEECCCCCCEEECCHHHHHHHHHH
FLGYIRGYDNAADFRELGTKTWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCC
ETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPCMHTHTFSLVGDTLHLTSYQR
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHH
SCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
CCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV
CCCCEEECCCHHHHHHHHHHEECCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA