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The map label for this gene is greB [H]

Identifier: 148827761

GI number: 148827761

Start: 1116006

End: 1116482

Strand: Direct

Name: greB [H]

Synonym: CGSHiGG_06120

Alternate gene names: 148827761

Gene position: 1116006-1116482 (Clockwise)

Preceding gene: 148827756

Following gene: 148827764

Centisome position: 59.14

GC content: 41.09

Gene sequence:

>477_bases
ATGGCAAAATCAAATTATATTACCCGTCAAGGCTGGCTTGCGCTTGACCAAGAACTCAAGTTTTTGTGGAAAGAAGAACG
CCCAAAAGTCACTCAAGCCGTTTCAGATGCAGCTGCGCTAGGCGATCGTAGCGAAAATGCCGAATATATTTATGGTAAAC
GTCGCTTACGTGAAATTGATCGCCGCGTGCGTTTTTTAACTAAAAGATTGGAAGTGCTACAAATTGTCGATTACAACCCA
AAGCAAGAGGGAAAAGTTTTCTTCGGTGCGTGGGTGGAATTAGAAAATGAAGAAGGTGAAATTAAGCAATACCGTATCGT
CGGTTGTGATGAATTTGCCCCTGCGAAAAATTGGATATCCATTGATTCCCCAGTTGCTCGAGCTTTAATCGGAAAAACAT
TAGACGACGAAGTGCGGGTAGAAACGCCATCAGGTTTTATCACACTTTATGTGAATAAAATTTGGTATGAAAAATAA

Upstream 100 bases:

>100_bases
TTAACATTGTCGTACTTCTTTATTATAAAAAAACGAAGATAGAATACTGGTTTTAGCGGGCTGGCTCAAATATAATTCAA
AAACTTTCTTTTATTTTATT

Downstream 100 bases:

>100_bases
CTTGGAAGAATAAAAGATAGCAAATACCTAAGCACAAAGAGAAAACTAATAGGCTAGATATAAACTATCTTACTTGCCTC
TTTCCTTATTCGCTGCAAAA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREIDRRVRFLTKRLEVLQIVDYNP
KQEGKVFFGAWVELENEEGEIKQYRIVGCDEFAPAKNWISIDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK

Sequences:

>Translated_158_residues
MAKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREIDRRVRFLTKRLEVLQIVDYNP
KQEGKVFFGAWVELENEEGEIKQYRIVGCDEFAPAKNWISIDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK
>Mature_157_residues
AKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREIDRRVRFLTKRLEVLQIVDYNPK
QEGKVFFGAWVELENEEGEIKQYRIVGCDEFAPAKNWISIDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=60.8974358974359, Blast_Score=188, Evalue=1e-49,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=37.5, Blast_Score=94, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18476; Mature: 18345

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREID
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RRVRFLTKRLEVLQIVDYNPKQEGKVFFGAWVELENEEGEIKQYRIVGCDEFAPAKNWIS
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCEE
IDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK
CCCHHHHHHHCCCCCCCEEEECCCCEEEEEEEEEECCC
>Mature Secondary Structure 
AKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREID
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RRVRFLTKRLEVLQIVDYNPKQEGKVFFGAWVELENEEGEIKQYRIVGCDEFAPAKNWIS
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCEE
IDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK
CCCHHHHHHHCCCCCCCEEEECCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]