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The map label for this gene is mazG [H]

Identifier: 148827657

GI number: 148827657

Start: 1007034

End: 1007825

Strand: Direct

Name: mazG [H]

Synonym: CGSHiGG_05540

Alternate gene names: 148827657

Gene position: 1007034-1007825 (Clockwise)

Preceding gene: 148827652

Following gene: 148827659

Centisome position: 53.36

GC content: 38.51

Gene sequence:

>792_bases
ATGCACTACTCTATTCAAGATTTTATTCAACTCATCGCCCAACTTCGCAATCCAAATGGCGGATGCCCTTGGGATTTAAA
ACAAAACTATGAATCCATGATTCCTTGTTTAACGGAAGAAACTTACGAAGTAATTGAAGCCATTGAGAAAAAAGACATAC
CAAATTTACGTGAAGAATTAGGGGATTTATTGTTGCAAGTCGTTTTCTTCAGCCAGCTTGCAACGGAAGATAAATACTTT
ACTTTTGACGATGTGTTACAAGATATCGCTGAAAAAATTGTACGCCGTCATCCTCACGTGTTTGGTGATGCAAAAGCGGG
GGACGAAACAGAAGCCCTTTCCCGTTGGAATGAAATGAAAGCCAAAGAAAAACAAGGTAAAAGTGAAGAAACCTCTATTT
TAGATAATGTGCCTCGTGCTTTGCCTTCTCTTACGCGAGCGGCAAAATTACAAAAACGTTGTTCAAAAGTAGGCTTTGAT
TGGGAAGAAATTTCACCCGTATTTGACAAAGTGCGGGAAGAATTAGAAGAAGTTCAAGCTGAAATTAACCGCACTTCGAT
TGAACAAAATAAAGTGGAAGAGGAAATCGGCGATTTATTGTTCGCAACCGTCAATCTTGCTCGCCACTTAAAATGTGATC
CTGAAGATGCATTGCGGAAAGCAAATTTAAAATTTGAACGTCGTTTTCGAGCAGTAGAGCAAGCGGTTCAACAACAAGGT
AAGCAAGTGAATAATGTGCCACTTATTGAATTAGATTTGTTATGGGATGAAGTGAAAAAACAAGAAAACTAA

Upstream 100 bases:

>100_bases
CACAAAGTATAAAGAAAATACGCCTAAAAAGCGACGGCATTTGCACCATTTTTCTTGTTCGTTATACTATCCAAAACATT
CCATCAAAGGAAATAAAATT

Downstream 100 bases:

>100_bases
TTGAAAAGTGCGGTAAAAATTTCTCTCATTTTTACCGCACTTTCTTCGTTAAAGTTGCTGAATATCCAGTGCCATTTGGT
CTAACATTTCATAACGTTTA

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MHYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMIPCLTEETYEVIEAIEKKDIPNLREELGDLLLQVVFFSQLATEDKYF
TFDDVLQDIAEKIVRRHPHVFGDAKAGDETEALSRWNEMKAKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFD
WEEISPVFDKVREELEEVQAEINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
KQVNNVPLIELDLLWDEVKKQEN

Sequences:

>Translated_263_residues
MHYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMIPCLTEETYEVIEAIEKKDIPNLREELGDLLLQVVFFSQLATEDKYF
TFDDVLQDIAEKIVRRHPHVFGDAKAGDETEALSRWNEMKAKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFD
WEEISPVFDKVREELEEVQAEINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
KQVNNVPLIELDLLWDEVKKQEN
>Mature_263_residues
MHYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMIPCLTEETYEVIEAIEKKDIPNLREELGDLLLQVVFFSQLATEDKYF
TFDDVLQDIAEKIVRRHPHVFGDAKAGDETEALSRWNEMKAKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFD
WEEISPVFDKVREELEEVQAEINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
KQVNNVPLIELDLLWDEVKKQEN

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=258, Percent_Identity=50, Blast_Score=272, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 30500; Mature: 30500

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMIPCLTEETYEVIEAIEKKDIPNLREEL
CCCCHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GDLLLQVVFFSQLATEDKYFTFDDVLQDIAEKIVRRHPHVFGDAKAGDETEALSRWNEMK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFDWEEISPVFDKVREELEEVQA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHC
KQVNNVPLIELDLLWDEVKKQEN
CCCCCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MHYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMIPCLTEETYEVIEAIEKKDIPNLREEL
CCCCHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GDLLLQVVFFSQLATEDKYFTFDDVLQDIAEKIVRRHPHVFGDAKAGDETEALSRWNEMK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFDWEEISPVFDKVREELEEVQA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHC
KQVNNVPLIELDLLWDEVKKQEN
CCCCCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]