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The map label for this gene is topB [H]

Identifier: 148827640

GI number: 148827640

Start: 989002

End: 990957

Strand: Direct

Name: topB [H]

Synonym: CGSHiGG_05445

Alternate gene names: 148827640

Gene position: 989002-990957 (Clockwise)

Preceding gene: 148827639

Following gene: 148827641

Centisome position: 52.41

GC content: 40.29

Gene sequence:

>1956_bases
ATGCGTTTATTTATCGCCGAAAAACCAAGCTTAGCTCGCGCTATTGCAGATGTGCTACCCAAACCCCATCAATGCGGGGA
TGGTTTTATTAAATGTGGCGATAATGATGTGGTAACTTGGTGTGTAGGGCATTTGCTCGAGCAAGCAGAACCCGATGCCT
ATGACCCTAAATTCAAACAATGGCGTTTAGAACATCTCCCTATTATTCCTGAAAAATGGCAACTTTTACCGCGAAAAGAA
GTAAAAAAACAGCTTTCTGTGGTGGAAAAACTGATCCATCAAGCGGATACGCTTGTTAATGCAGGAGACCCTGATAGAGA
AGGACAATTACTTGTAGATGAAGTGTTCAGTTATGCCAATTTATCTGCCGAAAAACGCGATAAAATTTTACGCTGCTTGA
TTAGCGACCTGAATCCAAGCGCGGTAGAAAAAGCGGTTAAAAAATTACAACCCAACCGTAATTTTATTCCTCTTGCCACA
TCCGCTCTCGCACGAGCTCGCGCCGATTGGCTTTATGGCATTAATATGACCAGAGCTTATACCATTCGAGGTAGACAAAC
TGGCTATGATGGCGTGCTTTCTGTTGGACGAGTGCAAACCCCTGTGCTTGGTTTAATTGTACGTCGAGATTTAGAAATTG
AACATTTCCAGCCCAAAGATTTCTTTGAAGTACTGGCTTGGGTTAATCCAGAGAGCAAAGAAGAAAAAACTCCAGAAAAA
TCAACCGCACTTTTCAGTGCCTTATGGCAACCCAGCAAAGCTTGTGAAGATTACCAAGATGATGACGGCAGGGTGCTCTC
TAAAGGATTAGCAGAAAACGTTGTAAAACGTATTACAAATCAACCTGCAGAAGTAACCGAATACAAAGACGTTCGTGAAA
AAGAAACAGCACCTTTGCCTTATTCCCTTTCTGCGTTACAAATTGATGCAGCAAAGCGATTCGGTATGTCCGCTCAAGCC
GTATTAGATACGTGCCAACGTCTATATGAAACTCATCGTTTAATTACTTATCCGCGTTCTGATTGTCGCTATTTGCCCGA
AGAACATTTTGCTGAACGACACAATGTATTAAATGCAATTTCAACCCATTGTGAAGCTTATCAACGCTTACCAAATGTCA
TTTCAACTGAACAGAGAAATCGCTGTTGGAATGATAAAAAAGTAGAGGCTCACCATGCAATCATTCCGACTGCCAAAAAT
CGTCCAGTAAATCTAACACAAGAAGAACGTAATATTTACAGCCTTATCGCTCGCCAATATTTAATGCAATTTTGTCCTGA
CGCAGAATATCGTAAAAGTAAAATCACATTAAATATCGCTGGCGGTACTTTTATAGCTCAAGCGCGAAATTTACAAACGG
CTGGGTGGAAAGAATTATTAGGCAAAGAAGACGACACAGAAAATCAAGAACCCTTATTACCAATAGTAAAGAAAGGACAA
ATTTTGCATTGTGAACGCGGAGAAGTGATGAGTAAAAAAACTCAGCCGCCAAAACCTTTCACCGATGCAACCTTGCTTTC
AGCAATGACGGGCATTGCACGATTTGTACAGAATAAAGAACTCAAAAAAATCCTGCGAGAAACCGATGGATTGGGTACAG
AGGCTACTCGCGCTGGCATCATTGAATTACTTTTTAAACGCGGTTTTTTAACGAAAAAAGGGCGAAATATTCATAGTACA
GAAGCTGGAAGAATTTTAATTCAGGCATTACCAGATATTGCCACCCAACCAGATATGACGGCTCATTGGGAATCGCAACT
CACAGACATTAGTCAAAAACAAGCAACCTATCAACAATTTATGCATAACTTGAATCAAATGTTGCCTGATTTAGTCCGTT
TTGTAGATCTTAATGCATTAAGACAATTAAGCCGCATTAAAATGATTAAATCCGATAGAGCGAAACCTAAAAGTGCGGTA
AAAAAATCCAGTAAATCAAACGGTCAAACTGATTAA

Upstream 100 bases:

>100_bases
CGGTATCCCTGTCGGAGGGGAACTTGAAACTGTGGACGGCACAACGCTTACTCACTCTTTTCTAGGTCGTCGTCAAATCG
ACTAATCTTCTCATACCATT

Downstream 100 bases:

>100_bases
AGTTAAACCAATTAATATTTTTTTTATGCAAAGCACTTGCGAGAAAATTGAATTTTCTATAACATTCACGACCAGTTTTC
TTGCAAAATTGCAAATTAGA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MRLFIAEKPSLARAIADVLPKPHQCGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQWRLEHLPIIPEKWQLLPRKE
VKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYANLSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLAT
SALARARADWLYGINMTRAYTIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVLAWVNPESKEEKTPEK
STALFSALWQPSKACEDYQDDDGRVLSKGLAENVVKRITNQPAEVTEYKDVREKETAPLPYSLSALQIDAAKRFGMSAQA
VLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAISTHCEAYQRLPNVISTEQRNRCWNDKKVEAHHAIIPTAKN
RPVNLTQEERNIYSLIARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQNKELKKILRETDGLGTEATRAGIIELLFKRGFLTKKGRNIHST
EAGRILIQALPDIATQPDMTAHWESQLTDISQKQATYQQFMHNLNQMLPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAV
KKSSKSNGQTD

Sequences:

>Translated_651_residues
MRLFIAEKPSLARAIADVLPKPHQCGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQWRLEHLPIIPEKWQLLPRKE
VKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYANLSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLAT
SALARARADWLYGINMTRAYTIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVLAWVNPESKEEKTPEK
STALFSALWQPSKACEDYQDDDGRVLSKGLAENVVKRITNQPAEVTEYKDVREKETAPLPYSLSALQIDAAKRFGMSAQA
VLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAISTHCEAYQRLPNVISTEQRNRCWNDKKVEAHHAIIPTAKN
RPVNLTQEERNIYSLIARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQNKELKKILRETDGLGTEATRAGIIELLFKRGFLTKKGRNIHST
EAGRILIQALPDIATQPDMTAHWESQLTDISQKQATYQQFMHNLNQMLPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAV
KKSSKSNGQTD
>Mature_651_residues
MRLFIAEKPSLARAIADVLPKPHQCGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQWRLEHLPIIPEKWQLLPRKE
VKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYANLSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLAT
SALARARADWLYGINMTRAYTIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVLAWVNPESKEEKTPEK
STALFSALWQPSKACEDYQDDDGRVLSKGLAENVVKRITNQPAEVTEYKDVREKETAPLPYSLSALQIDAAKRFGMSAQA
VLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAISTHCEAYQRLPNVISTEQRNRCWNDKKVEAHHAIIPTAKN
RPVNLTQEERNIYSLIARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQNKELKKILRETDGLGTEATRAGIIELLFKRGFLTKKGRNIHST
EAGRILIQALPDIATQPDMTAHWESQLTDISQKQATYQQFMHNLNQMLPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAV
KKSSKSNGQTD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=651, Percent_Identity=23.8095238095238, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788061, Length=648, Percent_Identity=65.4320987654321, Blast_Score=858, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=552, Percent_Identity=25, Blast_Score=109, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17555378, Length=636, Percent_Identity=21.8553459119497, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640096, Length=682, Percent_Identity=22.8739002932551, Blast_Score=102, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 74004; Mature: 74004

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFIAEKPSLARAIADVLPKPHQCGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQ
CEEEECCCCHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHH
WRLEHLPIIPEKWQLLPRKEVKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYAN
HHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
LSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLATSALARARADWLYGINMTRAY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCCEEEE
TIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVLAWVNPESKEEKTPEK
EECCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCHH
STALFSALWQPSKACEDYQDDDGRVLSKGLAENVVKRITNQPAEVTEYKDVREKETAPLP
HHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
YSLSALQIDAAKRFGMSAQAVLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAI
CCCHHHEEHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHHHHHHHHHHHH
STHCEAYQRLPNVISTEQRNRCWNDKKVEAHHAIIPTAKNRPVNLTQEERNIYSLIARQY
HHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHH
LMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
HHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCC
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQNKELKKILRETDGLGTEATRAGI
EEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IELLFKRGFLTKKGRNIHSTEAGRILIQALPDIATQPDMTAHWESQLTDISQKQATYQQF
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
MHNLNQMLPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAVKKSSKSNGQTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLFIAEKPSLARAIADVLPKPHQCGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQ
CEEEECCCCHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHH
WRLEHLPIIPEKWQLLPRKEVKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYAN
HHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
LSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLATSALARARADWLYGINMTRAY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCCEEEE
TIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVLAWVNPESKEEKTPEK
EECCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCHH
STALFSALWQPSKACEDYQDDDGRVLSKGLAENVVKRITNQPAEVTEYKDVREKETAPLP
HHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
YSLSALQIDAAKRFGMSAQAVLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAI
CCCHHHEEHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHHHHHHHHHHHH
STHCEAYQRLPNVISTEQRNRCWNDKKVEAHHAIIPTAKNRPVNLTQEERNIYSLIARQY
HHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHH
LMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
HHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCC
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQNKELKKILRETDGLGTEATRAGI
EEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IELLFKRGFLTKKGRNIHSTEAGRILIQALPDIATQPDMTAHWESQLTDISQKQATYQQF
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
MHNLNQMLPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAVKKSSKSNGQTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]