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The map label for this gene is pth [H]

Identifier: 148827596

GI number: 148827596

Start: 940033

End: 940617

Strand: Reverse

Name: pth [H]

Synonym: CGSHiGG_05145

Alternate gene names: 148827596

Gene position: 940617-940033 (Counterclockwise)

Preceding gene: 148827602

Following gene: 148827595

Centisome position: 49.84

GC content: 40.51

Gene sequence:

>585_bases
ATGTCAGAAATAAAACTCATTGTTGGTTTAGGCAATCCTGGCGAAAAATATGCCGATACACGCCATAATGCAGGCGAATG
GCTTATTGAACGCTTGGCTCGTCGTTTTAACGTTTCACTAAATCCAGAAAGTAAATTCTTCGGAAAAACTGTCCGCACTT
TAGTGAATGGAAAAGAAGTCCGCCTTTTAGTGCCAACAACTTTTATGAATTTAAGCGGTAAAGCGGTTGGGGCATTGGCA
AGTTTTTACCGTATTAAACCTGAAGAAATTTTGGTTATTCACGATGAACTAGATTTGCCTGCTGGCACAGCGAAACTCAA
ACAAGGCGGTGGGCACGGCGGACACAACGGCTTAAAAGATATTGTGGCTCAACTAGGCAATAACAATAATTTCTACCGTT
TACGCATTGGCATTGGGCATCCAGGCCATCGTGATTTAGTGGCAGGTTACGTCTTAAATAAACCATCTCCAGCAGATCGC
AATGCACTTGAAAAAGTGTTAGATGAGGCAACTGATTGTGTAGAAATGATTTTCAAAGATGGAATGATCAAAGCGACCAA
TCGTTTAAACAGTTTTAAAATTTAA

Upstream 100 bases:

>100_bases
ACCTCTTTGTTTCCGCCTTATAGATTGAGAATTTAAGGCTTTTTCGCTACAATACGCCACTATTTTTTACATCATCAAAT
TTTTACATCATCAAATAAAA

Downstream 100 bases:

>100_bases
AAGTGCGGTTAGTTTTACAACCGTTTTACTCAAAATAAGGAAAAATTATGGGATTCAAATGTGGTATTGTGGGATTGCCA
AATGTCGGCAAATCTACTCT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSEIKLIVGLGNPGEKYADTRHNAGEWLIERLARRFNVSLNPESKFFGKTVRTLVNGKEVRLLVPTTFMNLSGKAVGALA
SFYRIKPEEILVIHDELDLPAGTAKLKQGGGHGGHNGLKDIVAQLGNNNNFYRLRIGIGHPGHRDLVAGYVLNKPSPADR
NALEKVLDEATDCVEMIFKDGMIKATNRLNSFKI

Sequences:

>Translated_194_residues
MSEIKLIVGLGNPGEKYADTRHNAGEWLIERLARRFNVSLNPESKFFGKTVRTLVNGKEVRLLVPTTFMNLSGKAVGALA
SFYRIKPEEILVIHDELDLPAGTAKLKQGGGHGGHNGLKDIVAQLGNNNNFYRLRIGIGHPGHRDLVAGYVLNKPSPADR
NALEKVLDEATDCVEMIFKDGMIKATNRLNSFKI
>Mature_193_residues
SEIKLIVGLGNPGEKYADTRHNAGEWLIERLARRFNVSLNPESKFFGKTVRTLVNGKEVRLLVPTTFMNLSGKAVGALAS
FYRIKPEEILVIHDELDLPAGTAKLKQGGGHGGHNGLKDIVAQLGNNNNFYRLRIGIGHPGHRDLVAGYVLNKPSPADRN
ALEKVLDEATDCVEMIFKDGMIKATNRLNSFKI

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=171, Percent_Identity=37.4269005847953, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1787455, Length=190, Percent_Identity=64.2105263157895, Blast_Score=236, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6321983, Length=167, Percent_Identity=32.3353293413174, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 21360; Mature: 21229

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIKLIVGLGNPGEKYADTRHNAGEWLIERLARRFNVSLNPESKFFGKTVRTLVNGKEV
CCCEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEE
RLLVPTTFMNLSGKAVGALASFYRIKPEEILVIHDELDLPAGTAKLKQGGGHGGHNGLKD
EEEECCCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCHHHHH
IVAQLGNNNNFYRLRIGIGHPGHRDLVAGYVLNKPSPADRNALEKVLDEATDCVEMIFKD
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
GMIKATNRLNSFKI
CCHHHHHCCCCCCC
>Mature Secondary Structure 
SEIKLIVGLGNPGEKYADTRHNAGEWLIERLARRFNVSLNPESKFFGKTVRTLVNGKEV
CCEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEE
RLLVPTTFMNLSGKAVGALASFYRIKPEEILVIHDELDLPAGTAKLKQGGGHGGHNGLKD
EEEECCCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCHHHHH
IVAQLGNNNNFYRLRIGIGHPGHRDLVAGYVLNKPSPADRNALEKVLDEATDCVEMIFKD
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
GMIKATNRLNSFKI
CCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA