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The map label for this gene is gloA

Identifier: 148827472

GI number: 148827472

Start: 829904

End: 830311

Strand: Direct

Name: gloA

Synonym: CGSHiGG_04415

Alternate gene names: 148827472

Gene position: 829904-830311 (Clockwise)

Preceding gene: 148827471

Following gene: 148827473

Centisome position: 43.98

GC content: 40.44

Gene sequence:

>408_bases
ATGCAAATTTTACACACTATGTTACGCGTGGGCGATTTAGATCGTTCAATCAAATTTTATCAAGATGTTTTAGGTATGCG
TTTATTACGCACCAGTGAAAACCCAGAATACAAATATACGCTGGCTTTTTTAGGTTACGAAGATGGCGAAAGTGCGGCAG
AAATTGAATTAACATACAACTGGGGCGTAGATAAATATGAACACGGCACTGCATATGGACATATCGCTATCGGCGTAGAT
GATATTTATGCCACTTGTGAAGCCGTTCGCGCGAGCGGTGGTAACGTTACTCGCGAAGCAGGGCCAGTGAAAGGTGGATC
AACCGTCATTGCTTTTGTTGAAGATCCAGATGGTTATAAAATTGAATTTATCGAAAACAAAAGTACTAAATCTGGTTTAG
GCAACTAA

Upstream 100 bases:

>100_bases
TTTGGCTCGATGATTGCGGTTAAATAATTCATAACAAATTTATTTAATTCAACTTTTTTATGGCATAATGCCCGCACTTT
TATCAAAAAAGGACAAACCA

Downstream 100 bases:

>100_bases
AGTGCGGTCAATTTTTATTGTATTTTTAATAACGTAGGGCCTACGTTATTTTATTTTTAAGAGATAGATTATGTCCGATT
CTCAAGAAATCCCTTATCAC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MQILHTMLRVGDLDRSIKFYQDVLGMRLLRTSENPEYKYTLAFLGYEDGESAAEIELTYNWGVDKYEHGTAYGHIAIGVD
DIYATCEAVRASGGNVTREAGPVKGGSTVIAFVEDPDGYKIEFIENKSTKSGLGN

Sequences:

>Translated_135_residues
MQILHTMLRVGDLDRSIKFYQDVLGMRLLRTSENPEYKYTLAFLGYEDGESAAEIELTYNWGVDKYEHGTAYGHIAIGVD
DIYATCEAVRASGGNVTREAGPVKGGSTVIAFVEDPDGYKIEFIENKSTKSGLGN
>Mature_135_residues
MQILHTMLRVGDLDRSIKFYQDVLGMRLLRTSENPEYKYTLAFLGYEDGESAAEIELTYNWGVDKYEHGTAYGHIAIGVD
DIYATCEAVRASGGNVTREAGPVKGGSTVIAFVEDPDGYKIEFIENKSTKSGLGN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family

Homologues:

Organism=Homo sapiens, GI118402586, Length=146, Percent_Identity=34.9315068493151, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1787940, Length=135, Percent_Identity=74.0740740740741, Blast_Score=207, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323639, Length=136, Percent_Identity=41.9117647058824, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=36.4864864864865, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LGUL_HAEIN (P44638)

Other databases:

- EMBL:   L42023
- PIR:   I64147
- RefSeq:   NP_438488.1
- ProteinModelPortal:   P44638
- SMR:   P44638
- GeneID:   949438
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0323
- TIGR:   HI_0323
- HOGENOM:   HBG704761
- OMA:   PGPMKHG
- ProtClustDB:   CLSK808404
- BioCyc:   HINF71421:HI_0323-MONOMER
- BRENDA:   4.4.1.5
- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146
- TIGRFAMs:   TIGR00068

Pfam domain/function: PF00903 Glyoxalase

EC number: =4.4.1.5

Molecular weight: Translated: 14893; Mature: 14893

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00934 GLYOXALASE_I_1; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILHTMLRVGDLDRSIKFYQDVLGMRLLRTSENPEYKYTLAFLGYEDGESAAEIELTYN
CHHHHHHHHHCCCCCHHHHHHHHHHHHEEECCCCCCEEEEEEEEEECCCCCEEEEEEEEE
WGVDKYEHGTAYGHIAIGVDDIYATCEAVRASGGNVTREAGPVKGGSTVIAFVEDPDGYK
CCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCE
IEFIENKSTKSGLGN
EEEEECCCCCCCCCC
>Mature Secondary Structure
MQILHTMLRVGDLDRSIKFYQDVLGMRLLRTSENPEYKYTLAFLGYEDGESAAEIELTYN
CHHHHHHHHHCCCCCHHHHHHHHHHHHEEECCCCCCEEEEEEEEEECCCCCEEEEEEEEE
WGVDKYEHGTAYGHIAIGVDDIYATCEAVRASGGNVTREAGPVKGGSTVIAFVEDPDGYK
CCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCE
IEFIENKSTKSGLGN
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565