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The map label for this gene is ampD [H]

Identifier: 148827454

GI number: 148827454

Start: 813001

End: 813564

Strand: Direct

Name: ampD [H]

Synonym: CGSHiGG_04315

Alternate gene names: 148827454

Gene position: 813001-813564 (Clockwise)

Preceding gene: 148827449

Following gene: 148827455

Centisome position: 43.08

GC content: 37.06

Gene sequence:

>564_bases
ATGCAAAAACTAAAAGATATTGAACAAGGTATATTGACCGATTGTCGGCAAATTCCATCTCCGCATTTTGATAAACGCCC
AGATTTACAAGATATTTCACTTCTGGTGATCCATTACATTAGCTTACCGCCAGAACAATTTGGTGGCGGATATGTGGATG
ATTTTTTTCAAGGGAAATTAGATCCTAAGATTCATCCTTATTTTGCAGAAATTTATCAAATGCGTGTTTCTGCCCATTGT
TTGATCGAACGAAATGGGCGAATTACGCAATATGTCAATTTTAATGATCGCGCTTGGCACGCAGGCGTATCGAATTTTCA
AGGACGAGAAAAATGTAATGATTTTGCCATTGGAATTGAGCTGGAGGGGAGTAATGAACAGCCTTTTACCGACGCTCAAT
ATTTTTCATTGCAAGAACTTACCAATGTAATAATGAAAAGTTACCCGAAGATTACGAAAGATCGAATTGTTGGACATTGC
GATATTTCGCCAAAACGCAAGATCGATCCTGGTCAATATTTTGATTGGAAACGTTATTTATCCAGTGTAAAACAAACTGT
ATAA

Upstream 100 bases:

>100_bases
TTAAGCCTTTGTTGGCAACGACATTGTGAAAAGGAAAAATTTGTTTGTAAAATTGACCGCACTTTTTCTGCGATCCTGAT
CGCAAAAATAGGAAAAATGT

Downstream 100 bases:

>100_bases
AAATTAAACATCAAATTTTGATGTATTCTGTGTATTATAAATGCGTTCACTCTTTTACTTCTAACTAGTTGAATTAAAAG
GAATAAATTTAAATCTGAAA

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MQKLKDIEQGILTDCRQIPSPHFDKRPDLQDISLLVIHYISLPPEQFGGGYVDDFFQGKLDPKIHPYFAEIYQMRVSAHC
LIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIELEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHC
DISPKRKIDPGQYFDWKRYLSSVKQTV

Sequences:

>Translated_187_residues
MQKLKDIEQGILTDCRQIPSPHFDKRPDLQDISLLVIHYISLPPEQFGGGYVDDFFQGKLDPKIHPYFAEIYQMRVSAHC
LIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIELEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHC
DISPKRKIDPGQYFDWKRYLSSVKQTV
>Mature_187_residues
MQKLKDIEQGILTDCRQIPSPHFDKRPDLQDISLLVIHYISLPPEQFGGGYVDDFFQGKLDPKIHPYFAEIYQMRVSAHC
LIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIELEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHC
DISPKRKIDPGQYFDWKRYLSSVKQTV

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=173, Percent_Identity=57.8034682080925, Blast_Score=226, Evalue=9e-61,
Organism=Escherichia coli, GI1787092, Length=163, Percent_Identity=34.9693251533742, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21734; Mature: 21734

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLKDIEQGILTDCRQIPSPHFDKRPDLQDISLLVIHYISLPPEQFGGGYVDDFFQGKL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCCC
DPKIHPYFAEIYQMRVSAHCLIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIE
CCCCCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCHHHCCCCCCCCHHCCCCEEEEEE
LEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHCDISPKRKIDPGQYFDWKRYL
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCCCHHHHH
SSVKQTV
HHHHHCC
>Mature Secondary Structure
MQKLKDIEQGILTDCRQIPSPHFDKRPDLQDISLLVIHYISLPPEQFGGGYVDDFFQGKL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCCC
DPKIHPYFAEIYQMRVSAHCLIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIE
CCCCCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCHHHCCCCCCCCHHCCCCEEEEEE
LEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHCDISPKRKIDPGQYFDWKRYL
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCCCHHHHH
SSVKQTV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]