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The map label for this gene is hofB [H]

Identifier: 148827452

GI number: 148827452

Start: 811046

End: 812440

Strand: Reverse

Name: hofB [H]

Synonym: CGSHiGG_04305

Alternate gene names: 148827452

Gene position: 812440-811046 (Counterclockwise)

Preceding gene: 148827453

Following gene: 148827451

Centisome position: 43.05

GC content: 38.14

Gene sequence:

>1395_bases
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAATTGTTACAACAACTTACTCCGCGTCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACTTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAGAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGTGCGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAACCGCCTATTGCGT
ACTTTTTTACGCCAAGATCCCGATATCATTATGTTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACCGGGCATTTAGTACTCTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAACGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAATCAAGGAATGACGGATAATA
AAGAACTTGAACGTGTGCTAGGTAAAAACTCATGA

Upstream 100 bases:

>100_bases
AAGCTAAAGGTAATGCTACAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAACGGATGCCTCTTTATTTCCAGCAAAT
TTTTGCAGAAGTGTCACAAA

Downstream 100 bases:

>100_bases
CTAAAAAACTCTTTTATTATCAAGCTAGTAACGCATTAAATCAGGAACAAAAAGGCTCAATTATTGCGGATACAAAACAA
CAAGCACACTTTCAATTAAT

Product: protein transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFNRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYQGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS

Sequences:

>Translated_464_residues
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFNRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYQGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS
>Mature_463_residues
TSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPILL
ESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVLQ
PQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELGM
TENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFNRLLRT
FLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKCG
GNLINSCDCNQGYQGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS

Specific function: Unknown

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=387, Percent_Identity=45.2196382428941, Blast_Score=317, Evalue=7e-88,
Organism=Escherichia coli, GI1789723, Length=389, Percent_Identity=41.9023136246787, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI87082188, Length=280, Percent_Identity=33.2142857142857, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 52927; Mature: 52796

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLS
CCCEEEEEEEEEECCCCCEEEECCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
NLSACETIAFITGKPVEPILLESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDE
CCHHHHEEEEECCCCCCHHEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVLQPQPLISKIFANRIISRLKL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
HHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEHHHCC
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELD
CCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHCCCCCCCEEECCCCCEEEEC
GIIQSQINPQIGLDFNRLLRTFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHT
CHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEECC
NDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKCGGNLINSCDCNQGYQGRIGV
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
YQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLS
CCEEEEEEEEEECCCCCEEEECCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
NLSACETIAFITGKPVEPILLESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDE
CCHHHHEEEEECCCCCCHHEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVLQPQPLISKIFANRIISRLKL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
HHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEHHHCC
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELD
CCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHCCCCCCCEEECCCCCEEEEC
GIIQSQINPQIGLDFNRLLRTFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHT
CHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEECC
NDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKCGGNLINSCDCNQGYQGRIGV
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
YQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]