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The map label for this gene is pyrE

Identifier: 148827426

GI number: 148827426

Start: 783955

End: 784596

Strand: Reverse

Name: pyrE

Synonym: CGSHiGG_04160

Alternate gene names: 148827426

Gene position: 784596-783955 (Counterclockwise)

Preceding gene: 148827427

Following gene: 148827425

Centisome position: 41.57

GC content: 41.43

Gene sequence:

>642_bases
ATGGAACAATATAAACGCGATTTTATCGAATTTGCTTTAAGCCGAAATGTATTGAAATTCGGCGAGTTTACTTTGAAATC
GGGGCGTAAAAGTCCGTATTTCTTCAATGCGGGTTTGTTCAATACGGGGGCAGATTTAGCACGTTTAGGCGAGTTTTATG
CTGCGGCAATTCAAGCAAGTGCGGTAGATTTTGATGTGGTTTTTGGCCCTGCTTACAAAGGTATTCCAATTGGCACGTCA
GTTTCGGTGGCGTTATTTAATCGTTATGGTATTGATAAACCCGTGTGTTTTAATCGCAAAGAAGTGAAAGATCACGGAGA
GGGCGGCAATTTAATTGGTAGCCCATTGCAAGGAAAAATTTTGCTTGTGGATGACGTGATTACGGCTGGCACTGCAATTC
GTGAATCTATGGAATTAATCAGTGCAAATAAAGCAGAGCTTGCGGCGGTATTGATCGCTCTAAACCGTAAGGAACGAGGA
AAAGGCGAGCTTTCAGCAATTCAAGAAGTTGAACGTGATTATCAATGCCAAGTATTATCCATCATTGATTTAGATGACTT
GATGCAATTTATCGAACAAGATCCTCGATACAGTAGCCATTTGCCAGAAATGCGTGCTTATCGTGCCGAATTTGGCGTAT
AA

Upstream 100 bases:

>100_bases
TATTAACATTATTAGATTTAGCGAAACAAGGCTGTAATCAAATTTTTATCGCTCAACGCAAAGTGCTAGGCTTATAAACA
CAGTTTATAGGAGTTAAAAA

Downstream 100 bases:

>100_bases
AACTTGAGAAATAAATTTTTATCATCATTATAAAAATAACCGCACTTTGACTTAACAGAGGAAAGAATGGAATTTATTGG
AAAAATTATAGGCGTATTTT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQASAVDFDVVFGPAYKGIPIGTS
VSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKILLVDDVITAGTAIRESMELISANKAELAAVLIALNRKERG
KGELSAIQEVERDYQCQVLSIIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV

Sequences:

>Translated_213_residues
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQASAVDFDVVFGPAYKGIPIGTS
VSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKILLVDDVITAGTAIRESMELISANKAELAAVLIALNRKERG
KGELSAIQEVERDYQCQVLSIIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV
>Mature_213_residues
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQASAVDFDVVFGPAYKGIPIGTS
VSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKILLVDDVITAGTAIRESMELISANKAELAAVLIALNRKERG
KGELSAIQEVERDYQCQVLSIIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=180, Percent_Identity=26.1111111111111, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=69.0140845070423, Blast_Score=314, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17508631, Length=162, Percent_Identity=30.8641975308642, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554964, Length=190, Percent_Identity=27.3684210526316, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=200, Percent_Identity=49.5, Blast_Score=181, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6323927, Length=225, Percent_Identity=44, Blast_Score=174, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_HAEI8 (Q4QNR7)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_247990.1
- HSSP:   P08870
- ProteinModelPortal:   Q4QNR7
- SMR:   Q4QNR7
- STRING:   Q4QNR7
- GeneID:   3429704
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0380
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- PhylomeDB:   Q4QNR7
- ProtClustDB:   PRK00455
- BioCyc:   HINF281310:NTHI0380-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23643; Mature: 23643

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 99-99 BINDING 100-100 BINDING 103-103 BINDING 105-105 BINDING 128-128 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQAS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
AVDFDVVFGPAYKGIPIGTSVSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKI
CCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCE
LLVDDVITAGTAIRESMELISANKAELAAVLIALNRKERGKGELSAIQEVERDYQCQVLS
EEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEE
IIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV
EECHHHHHHHHHCCCCHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQAS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
AVDFDVVFGPAYKGIPIGTSVSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKI
CCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCE
LLVDDVITAGTAIRESMELISANKAELAAVLIALNRKERGKGELSAIQEVERDYQCQVLS
EEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEE
IIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV
EECHHHHHHHHHCCCCHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA