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The map label for this gene is rpoH [H]

Identifier: 148827424

GI number: 148827424

Start: 781710

End: 782555

Strand: Direct

Name: rpoH [H]

Synonym: CGSHiGG_04140

Alternate gene names: 148827424

Gene position: 781710-782555 (Clockwise)

Preceding gene: 148827423

Following gene: 148827429

Centisome position: 41.42

GC content: 40.31

Gene sequence:

>846_bases
ATGGATAAAGAAACACAAATGATGTTAGTTCCTCAAGGCAGTATCGAAGGTTATATTCGTGCAGCAAATGAATATCCGAT
GCTGACCGCAGAGGAAGAAAAGGAATTGGCAGAACGTTTGTATTATCACGAGGATTTAGATGCAGCGAAAAAACTCATTT
TGTCGCATCTTCGTTTCGTGATCCACGTGGCGCGTGGCTATTCTGGCTATGGTTTGCCACAAGCTGACTTAATCCAAGAA
GGCAATATCGGCTTAATGAAAGCGGTCAAACGTTTTAACCCTGAAGTTGGTGTACGCTTGGTGTCATTCGCTGTGCATTG
GATTAAAGCTGAGATCCACGAATATGTTCTTCGTAACTGGCGTATCGTAAAAGTCGCAACCACGAAAGCTCAACGTAAAT
TATTTTTCAACCTACGTAAAACTAAACAACGTTTAGGTTGGTTCAATGAAAATGAAGTTGATATGGTCGCAAATGAATTG
GGTGTATCCAAAGAAGATGTGATCGAAATGGAGTCTCGAATGAGCGGTGCGGATGTCGGTTTTGATTTACCTACTGATGA
TGCAGAAACAGAGACCTATTCTCCAGCCCTTTATTTGGAAGATAAAAGTTCTAATTTCGCAGCAGAGCTTGAAAATGAAA
ACTTTGAAAGCCAAGCAACCGAGCAATTAAGTGCGGCGCTACAAAGTCTAGATGCACGTAGTCAGGATATTATCAAAGCT
CGTTGGTTAGATGATAACAAAGCTACATTGCACGATTTGGCAGCAAAATATAATGTCTCTGCGGAACGTATTCGTCAATT
AGAAACTAATGCACTGAAAAAATTAAAAAGTGCGGTGAATTTTTAA

Upstream 100 bases:

>100_bases
GATAAAATCTGTCTTATTAACAAATTGGTTCAATAAGATAGCTTATTGTGCTATAACTAAGTACATTGATTGGCAAAAGC
CAAAATAAAGAAGGGAAACG

Downstream 100 bases:

>100_bases
ATAATTTAGAAAAAGAAAACCTGCTGATTAGCAGGTTTTTTATTTATCATTTAACCGTAATTTCAAATCTTCAGCAGTTT
GGCAGGTCTTAATCTTATCA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFVIHVARGYSGYGLPQADLIQE
GNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANEL
GVSKEDVIEMESRMSGADVGFDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLSAALQSLDARSQDIIKA
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF

Sequences:

>Translated_281_residues
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFVIHVARGYSGYGLPQADLIQE
GNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANEL
GVSKEDVIEMESRMSGADVGFDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLSAALQSLDARSQDIIKA
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF
>Mature_281_residues
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFVIHVARGYSGYGLPQADLIQE
GNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANEL
GVSKEDVIEMESRMSGADVGFDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLSAALQSLDARSQDIIKA
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=71.0714285714286, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1789098, Length=280, Percent_Identity=27.8571428571429, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789448, Length=257, Percent_Identity=29.5719844357977, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32064; Mature: 32064

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFV
CCCCCCEEEEECCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IHVARGYSGYGLPQADLIQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNW
HHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANELGVSKEDVIEMESRMSGADVG
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
FDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLSAALQSLDARSQDIIKA
CCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFV
CCCCCCEEEEECCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IHVARGYSGYGLPQADLIQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNW
HHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANELGVSKEDVIEMESRMSGADVG
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
FDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLSAALQSLDARSQDIIKA
CCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]