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The map label for this gene is mepA [H]

Identifier: 148827336

GI number: 148827336

Start: 670042

End: 670902

Strand: Direct

Name: mepA [H]

Synonym: CGSHiGG_03595

Alternate gene names: 148827336

Gene position: 670042-670902 (Clockwise)

Preceding gene: 148827335

Following gene: 148827337

Centisome position: 35.5

GC content: 43.21

Gene sequence:

>861_bases
ATGAATAAAATTTTATTAAAAACAACCATAATTTTTACCGCACTTTTTTCTCTGAACGTTGTTGCCTCTCCTCTAGATTG
GCAAAAAGTGAAACGTCCAATACCAAGTGACGATGGGAAAGCAAAACCTATTGGAAGTTATACCAATGGATGTATTATTG
GGGCGCAAGCATTACCAGCGAAAGGTGAAGGTTACCAAGTGATTCGTATGAATCGCAATCGCTATTATGGCCATCCAAAT
ATGATTCTGTATCTTGAACGTTTGGGACAACGCGTTAAAGCTGCTGGGTTGCCAACTATGTTAGTGGGCGATATTGCCAT
GCCGGGTGGCGGACGGTTTTTAACGGGTCATGCTAGCCATCAGATGGGATTAGATGCGGATATTTGGTTAAGAATGGGTG
GAATGTCAGATACCGATGCACTGAATTCTGATGGTAAAGGTTTATTAGTTGTAGATAGAAAGGCACAACGAGTAGATGAG
CGCGTGTGGAATAGCAATCACGCGACGTTAATCAAATTAGCCGCGCAAGATCCAAATGTGACGCGTATTTTTGTTAATCC
TGCGATTAAGGTAAAATTATGCCAAACCGCTGGCAATGATCGCGGTTGGCTACACAAAGTTCGTCCTTGGTATGGACACG
ACTCTCATTTTCATGTTCGTTTAACTTGTCCCGCAGATGCGCCTTATTGTGAAAATCAAGCGCCTGTGCCTGCCGGTGAT
GGTTGTGGAGACGAATTATATTCTTGGTTTGAACCGCCAAAACCAGGTGCTTCAGTGAGCAAACCTAAAGTGACACCACC
GGAACCATTTTTGTGCCAACAAATTTTGAACTCACCGAATCGGAGTGAATGGTTAGAATAG

Upstream 100 bases:

>100_bases
TAGAGTATAAAGTTCCCCAAAGGGGAACTATTTTTCTAACCGTGGGTATACTTACCCACGGTAAAACCTAGGATATATTT
ATCTACAGCAAAACTTTAGA

Downstream 100 bases:

>100_bases
CATTAAGGTAAATCAATATGGATATCGGCATTGATCTTTTAGCAATATTGTTTTGTGTTGGTTTTGTTGCATCATTTATC
GATGCAATTGCTGGCGGTGG

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSDDGKAKPIGSYTNGCIIGAQALPAKGEGYQVIRMNRNRYYGHPN
MILYLERLGQRVKAAGLPTMLVGDIAMPGGGRFLTGHASHQMGLDADIWLRMGGMSDTDALNSDGKGLLVVDRKAQRVDE
RVWNSNHATLIKLAAQDPNVTRIFVNPAIKVKLCQTAGNDRGWLHKVRPWYGHDSHFHVRLTCPADAPYCENQAPVPAGD
GCGDELYSWFEPPKPGASVSKPKVTPPEPFLCQQILNSPNRSEWLE

Sequences:

>Translated_286_residues
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSDDGKAKPIGSYTNGCIIGAQALPAKGEGYQVIRMNRNRYYGHPN
MILYLERLGQRVKAAGLPTMLVGDIAMPGGGRFLTGHASHQMGLDADIWLRMGGMSDTDALNSDGKGLLVVDRKAQRVDE
RVWNSNHATLIKLAAQDPNVTRIFVNPAIKVKLCQTAGNDRGWLHKVRPWYGHDSHFHVRLTCPADAPYCENQAPVPAGD
GCGDELYSWFEPPKPGASVSKPKVTPPEPFLCQQILNSPNRSEWLE
>Mature_286_residues
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSDDGKAKPIGSYTNGCIIGAQALPAKGEGYQVIRMNRNRYYGHPN
MILYLERLGQRVKAAGLPTMLVGDIAMPGGGRFLTGHASHQMGLDADIWLRMGGMSDTDALNSDGKGLLVVDRKAQRVDE
RVWNSNHATLIKLAAQDPNVTRIFVNPAIKVKLCQTAGNDRGWLHKVRPWYGHDSHFHVRLTCPADAPYCENQAPVPAGD
GCGDELYSWFEPPKPGASVSKPKVTPPEPFLCQQILNSPNRSEWLE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=252, Percent_Identity=51.5873015873016, Blast_Score=276, Evalue=9e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 31549; Mature: 31549

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSDDGKAKPIGSYTNGCIIGAQALPA
CCCEEHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
KGEGYQVIRMNRNRYYGHPNMILYLERLGQRVKAAGLPTMLVGDIAMPGGGRFLTGHASH
CCCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCC
QMGLDADIWLRMGGMSDTDALNSDGKGLLVVDRKAQRVDERVWNSNHATLIKLAAQDPNV
CCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCE
TRIFVNPAIKVKLCQTAGNDRGWLHKVRPWYGHDSHFHVRLTCPADAPYCENQAPVPAGD
EEEEECCCEEEEEEECCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCC
GCGDELYSWFEPPKPGASVSKPKVTPPEPFLCQQILNSPNRSEWLE
CCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSDDGKAKPIGSYTNGCIIGAQALPA
CCCEEHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
KGEGYQVIRMNRNRYYGHPNMILYLERLGQRVKAAGLPTMLVGDIAMPGGGRFLTGHASH
CCCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCC
QMGLDADIWLRMGGMSDTDALNSDGKGLLVVDRKAQRVDERVWNSNHATLIKLAAQDPNV
CCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCE
TRIFVNPAIKVKLCQTAGNDRGWLHKVRPWYGHDSHFHVRLTCPADAPYCENQAPVPAGD
EEEEECCCEEEEEEECCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCC
GCGDELYSWFEPPKPGASVSKPKVTPPEPFLCQQILNSPNRSEWLE
CCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]