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The map label for this gene is menE [H]

Identifier: 148827333

GI number: 148827333

Start: 664066

End: 665424

Strand: Direct

Name: menE [H]

Synonym: CGSHiGG_03580

Alternate gene names: 148827333

Gene position: 664066-665424 (Clockwise)

Preceding gene: 148827332

Following gene: 148827334

Centisome position: 35.19

GC content: 36.72

Gene sequence:

>1359_bases
ATGTATCCTTGGCAAGATTTTGCTATTCAACCTGATTTCTCAGATAAAATCGCTTTGCATACTACGCAGGGCGATGTGCT
TACTTGGATAGAATTGACTACAAAGATTAACCAAACAGTGGCTTTTTTACAAAAAAAAGGCGTAAATGCGGAAAGTGCGG
TTGCTTTTGTGGGAAAAAATTCAGAGAAAATTTTATTTTTATATCTGGCGACAATTCAGCTTGGCGCAAAAGTTTTAGGC
ATAAACCCTGCTTTTCCACAAGAAAAAATTGCAAAATTATGTGAGTTTTATCAAATCGATTTTTGTTTTTATGATGAAGA
TTTGAGGGATTTTCCAAAAGCTGACGTAGTTATACAAAAAGCTGATTTCTTTCGCCCTGCAACAATGACGCTAACGTCTG
GTTCTACAGGCTTACCAAAAGCAGTTGTGCATAATGTCCAAGCACATTTGGATAATGCAAAAGGGGTATGTAACTTAATG
AAGTTTGATCGTAATCAATCTTGGTTACTTTCATTACCCTTATATCACGTTTCAGGGCAAGGTATTGTTTGGCGTTGGTT
ATATTGCGGTGCACAATTACATTTCCCAGAAGATGATTTTTATGCTTCATTATTAAAGACGACCCACGTTTCTCTTGTGC
CAACGCAATTACAGCGTTTATTAGATTATTTACAGGAAAATCCGACGGTTTCATTTTCTACACGCCATATTTTACTTGGT
GGTACGCATATTCCTGCTGAACTCACGGAAAAAATAGTGAAATATGGTATCGAAACATATTCTGGCTATGGAATGACGGA
AATGGCTTCAACAGTTTTTGCTAAAAAATCTGACGGAAAACAAGGCGTAGGGCAACCGCTCTTAGGTAGAGAGTATTGTT
TAGTAAATGATGAAATTTGGCTGAAAGGTGCAGGTTTGGCGATGGGTTATTGGAAAGATCGACAAATTGTTCCATTAACG
AATAACCAAGGCTGGATTCAGACAAAAGATAAAGGCATTTGGCAAGAGGGCGAACTTGTTATTATCGGACGACTTGATAA
TATGTTTATTTCAGGTGGCGAAAATATTCAGCCAGAAGAAATTGAACAAGTGATTATTCAACATTCTTCAGTTAATCAAG
TGTTTGTTTTACCACAAAAAAACAAAGAATTTGGTCAGCGTCCTGTCGCTTTAGTGGATTTTAATGAACCCTTTAGCAAA
AGTGCGGTTGAAAATTTAATGTTTTTTTTACAAGATAAACTTGCACGTTTTAAACAACCTATTGCATATTATCCTTTGCC
ACCGATGCTTGAGAAAGGCATTAAGATTTCACGTAAACAGCTTGCTGATTGGCTGGCAAAGCGAGATGAGATAAATTAA

Upstream 100 bases:

>100_bases
GTGGACGAAAAATGTTAATGCTAGAAGGCGCAATGCAATCTATGGAATTACCTGAAACATTGATTGATGAAGTGCGATCA
TATTTCACGGTAAATTAATA

Downstream 100 bases:

>100_bases
TTTACCATTTAGATTAGGGAAAAATAATGATTAGGAAACTTATGAAAATACCTCCATTTTTTACCGCACTTTTTGCCTCG
GCAATGTTTACCCTTAGCGT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MYPWQDFAIQPDFSDKIALHTTQGDVLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKNSEKILFLYLATIQLGAKVLG
INPAFPQEKIAKLCEFYQIDFCFYDEDLRDFPKADVVIQKADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLM
KFDRNQSWLLSLPLYHVSGQGIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPTVSFSTRHILLG
GTHIPAELTEKIVKYGIETYSGYGMTEMASTVFAKKSDGKQGVGQPLLGREYCLVNDEIWLKGAGLAMGYWKDRQIVPLT
NNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEEIEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSK
SAVENLMFFLQDKLARFKQPIAYYPLPPMLEKGIKISRKQLADWLAKRDEIN

Sequences:

>Translated_452_residues
MYPWQDFAIQPDFSDKIALHTTQGDVLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKNSEKILFLYLATIQLGAKVLG
INPAFPQEKIAKLCEFYQIDFCFYDEDLRDFPKADVVIQKADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLM
KFDRNQSWLLSLPLYHVSGQGIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPTVSFSTRHILLG
GTHIPAELTEKIVKYGIETYSGYGMTEMASTVFAKKSDGKQGVGQPLLGREYCLVNDEIWLKGAGLAMGYWKDRQIVPLT
NNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEEIEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSK
SAVENLMFFLQDKLARFKQPIAYYPLPPMLEKGIKISRKQLADWLAKRDEIN
>Mature_452_residues
MYPWQDFAIQPDFSDKIALHTTQGDVLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKNSEKILFLYLATIQLGAKVLG
INPAFPQEKIAKLCEFYQIDFCFYDEDLRDFPKADVVIQKADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLM
KFDRNQSWLLSLPLYHVSGQGIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPTVSFSTRHILLG
GTHIPAELTEKIVKYGIETYSGYGMTEMASTVFAKKSDGKQGVGQPLLGREYCLVNDEIWLKGAGLAMGYWKDRQIVPLT
NNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEEIEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSK
SAVENLMFFLQDKLARFKQPIAYYPLPPMLEKGIKISRKQLADWLAKRDEIN

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=343, Percent_Identity=25.3644314868805, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788595, Length=458, Percent_Identity=43.8864628820961, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI145693145, Length=484, Percent_Identity=22.9338842975207, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788107, Length=348, Percent_Identity=27.0114942528736, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786810, Length=384, Percent_Identity=23.1770833333333, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=470, Percent_Identity=27.8723404255319, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=433, Percent_Identity=25.8660508083141, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=350, Percent_Identity=24.8571428571429, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=350, Percent_Identity=24.8571428571429, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=350, Percent_Identity=24.8571428571429, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=346, Percent_Identity=26.5895953757225, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=332, Percent_Identity=25.9036144578313, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=336, Percent_Identity=25, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=347, Percent_Identity=25.6484149855908, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24581924, Length=331, Percent_Identity=25.6797583081571, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355181, Length=423, Percent_Identity=21.7494089834515, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51250; Mature: 51250

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00455 AMP_BINDING ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPWQDFAIQPDFSDKIALHTTQGDVLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKN
CCCCCCCEECCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHEEEEECCC
SEKILFLYLATIQLGAKVLGINPAFPQEKIAKLCEFYQIDFCFYDEDLRDFPKADVVIQK
CCEEEEEEEEHHHHCCEEEECCCCCCHHHHHHHHHHHEEEEEEECCHHHHCCCCCEEEEE
ADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLMKFDRNQSWLLSLPLYHVSGQ
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEEECCC
GIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPTVSFSTRHILLG
CEEEEEEEECCEEECCCHHHHHHHHHHHHHEECHHHHHHHHHHHHCCCCEEEECCEEEEE
GTHIPAELTEKIVKYGIETYSGYGMTEMASTVFAKKSDGKQGVGQPLLGREYCLVNDEIW
CCCCCHHHHHHHHHHCCHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEE
LKGAGLAMGYWKDRQIVPLTNNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEE
EEECCEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCHHH
IEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSKSAVENLMFFLQDKLARFKQP
HHHHHHHCCCCCEEEEEECCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
IAYYPLPPMLEKGIKISRKQLADWLAKRDEIN
CCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYPWQDFAIQPDFSDKIALHTTQGDVLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKN
CCCCCCCEECCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHEEEEECCC
SEKILFLYLATIQLGAKVLGINPAFPQEKIAKLCEFYQIDFCFYDEDLRDFPKADVVIQK
CCEEEEEEEEHHHHCCEEEECCCCCCHHHHHHHHHHHEEEEEEECCHHHHCCCCCEEEEE
ADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLMKFDRNQSWLLSLPLYHVSGQ
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEEECCC
GIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPTVSFSTRHILLG
CEEEEEEEECCEEECCCHHHHHHHHHHHHHEECHHHHHHHHHHHHCCCCEEEECCEEEEE
GTHIPAELTEKIVKYGIETYSGYGMTEMASTVFAKKSDGKQGVGQPLLGREYCLVNDEIW
CCCCCHHHHHHHHHHCCHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEE
LKGAGLAMGYWKDRQIVPLTNNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEE
EEECCEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCHHH
IEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSKSAVENLMFFLQDKLARFKQP
HHHHHHHCCCCCEEEEEECCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
IAYYPLPPMLEKGIKISRKQLADWLAKRDEIN
CCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]