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The map label for this gene is ybfF [C]

Identifier: 148827331

GI number: 148827331

Start: 662617

End: 663399

Strand: Reverse

Name: ybfF [C]

Synonym: CGSHiGG_03570

Alternate gene names: 148827331

Gene position: 663399-662617 (Counterclockwise)

Preceding gene: 148827339

Following gene: 148827330

Centisome position: 35.15

GC content: 33.46

Gene sequence:

>783_bases
ATGGCAAAATCATTACTCAATTATCAATTTCATCAAGTAAAACAAACGATTAATACACCTGTTTTAATTTTTATTCACGG
TTTATTCGGCGATATGGATAATCTCGGCGTTATTGCGAGAGCTTTTAGTGAACATTACAGTATTTTACGTATTGATTTAC
GCAATCACGGCCATAGTTTTCATTCGGAAAAAATGAATTATCAACTGATGGCTGAAGATGTTATCGCAGTAATCCGCCAC
TTAAATTTATCCAAAGTGATATTAATTGGTCATTCTATGGGGGGAAAAACCGCCATGAAAATCACCGCACTTTGCCCAGA
ATTAGTCGAAAAATTAATCGTAATTGATATGTCCCCTCTGCCTTATGAAGGATTTGGTCATAAAGATGTGTTTAATGGCC
TATTTGCGGTAAAAAATGCAAAGCCTGAAAATCGTCAGCAAGCCAAACCGATTTTAAAACAAGAAATTAATGATGAAGAC
GTTGTACAATTTATGTTGAAATCTTTTGATGTTAATTCAGCTGATTGTTTTCGTTTTAATCTTACCGCACTTTTTAACAA
TTATGCCAATATTATGGATTGGGAAAAAGTACGTGTATTTACGCCAACTCTTTTTATTAAGGGTGGAAATTCCTCTTATA
TAAAAATAGAAAATAGCGAAAAAATTCTAGAACAATTTCCTAATGCAACTGCCTTTACGATTAATGGCAGTGGACACTGG
GTTCACGCCGAAAAACCTGATTTTGTTATTCGTGCAATTAAACGATTTCTAATTAAGAATTAA

Upstream 100 bases:

>100_bases
TAATTCTTCATCTACTTCAATGATCTTCATTTTTATCTCTCTGTTTGCTAAAATTTTTTTCAATTATAACGACTTCTTTA
CAAATTCTCACGTAAAAATT

Downstream 100 bases:

>100_bases
GTAGTTTAAGTGAGAATGCTTTTATGATATAGTTCGCCAAATTTGGATAAGCCTTATAATCAATAGATTAAATTTAATAT
AAAAAAGGAAATAAAAATGG

Product: esterase/lipase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MAKSLLNYQFHQVKQTINTPVLIFIHGLFGDMDNLGVIARAFSEHYSILRIDLRNHGHSFHSEKMNYQLMAEDVIAVIRH
LNLSKVILIGHSMGGKTAMKITALCPELVEKLIVIDMSPLPYEGFGHKDVFNGLFAVKNAKPENRQQAKPILKQEINDED
VVQFMLKSFDVNSADCFRFNLTALFNNYANIMDWEKVRVFTPTLFIKGGNSSYIKIENSEKILEQFPNATAFTINGSGHW
VHAEKPDFVIRAIKRFLIKN

Sequences:

>Translated_260_residues
MAKSLLNYQFHQVKQTINTPVLIFIHGLFGDMDNLGVIARAFSEHYSILRIDLRNHGHSFHSEKMNYQLMAEDVIAVIRH
LNLSKVILIGHSMGGKTAMKITALCPELVEKLIVIDMSPLPYEGFGHKDVFNGLFAVKNAKPENRQQAKPILKQEINDED
VVQFMLKSFDVNSADCFRFNLTALFNNYANIMDWEKVRVFTPTLFIKGGNSSYIKIENSEKILEQFPNATAFTINGSGHW
VHAEKPDFVIRAIKRFLIKN
>Mature_259_residues
AKSLLNYQFHQVKQTINTPVLIFIHGLFGDMDNLGVIARAFSEHYSILRIDLRNHGHSFHSEKMNYQLMAEDVIAVIRHL
NLSKVILIGHSMGGKTAMKITALCPELVEKLIVIDMSPLPYEGFGHKDVFNGLFAVKNAKPENRQQAKPILKQEINDEDV
VQFMLKSFDVNSADCFRFNLTALFNNYANIMDWEKVRVFTPTLFIKGGNSSYIKIENSEKILEQFPNATAFTINGSGHWV
HAEKPDFVIRAIKRFLIKN

Specific function: Unknown

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=249, Percent_Identity=34.9397590361446, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI23200012, Length=249, Percent_Identity=34.9397590361446, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI223941839, Length=244, Percent_Identity=29.5081967213115, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1786902, Length=253, Percent_Identity=43.0830039525692, Blast_Score=230, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17508535, Length=255, Percent_Identity=32.9411764705882, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17507063, Length=268, Percent_Identity=28.3582089552239, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17554012, Length=274, Percent_Identity=26.2773722627737, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321468, Length=266, Percent_Identity=31.203007518797, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640348, Length=258, Percent_Identity=32.5581395348837, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24646055, Length=261, Percent_Identity=22.6053639846743, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR012908 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07819 PGAP1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 29669; Mature: 29538

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSLLNYQFHQVKQTINTPVLIFIHGLFGDMDNLGVIARAFSEHYSILRIDLRNHGHSF
CCCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHHHHHHCCEEEEEEECCCCCCH
HSEKMNYQLMAEDVIAVIRHLNLSKVILIGHSMGGKTAMKITALCPELVEKLIVIDMSPL
HHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCCC
PYEGFGHKDVFNGLFAVKNAKPENRQQAKPILKQEINDEDVVQFMLKSFDVNSADCFRFN
CCCCCCCHHHHHHHHEECCCCCCCHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCEEEEE
LTALFNNYANIMDWEKVRVFTPTLFIKGGNSSYIKIENSEKILEQFPNATAFTINGSGHW
HHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEECCHHHHHHHCCCCEEEEECCCCEE
VHAEKPDFVIRAIKRFLIKN
EECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKSLLNYQFHQVKQTINTPVLIFIHGLFGDMDNLGVIARAFSEHYSILRIDLRNHGHSF
CCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHHHHHHCCEEEEEEECCCCCCH
HSEKMNYQLMAEDVIAVIRHLNLSKVILIGHSMGGKTAMKITALCPELVEKLIVIDMSPL
HHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCCC
PYEGFGHKDVFNGLFAVKNAKPENRQQAKPILKQEINDEDVVQFMLKSFDVNSADCFRFN
CCCCCCCHHHHHHHHEECCCCCCCHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCEEEEE
LTALFNNYANIMDWEKVRVFTPTLFIKGGNSSYIKIENSEKILEQFPNATAFTINGSGHW
HHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEECCHHHHHHHCCCCEEEEECCCCEE
VHAEKPDFVIRAIKRFLIKN
EECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]