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The map label for this gene is apbE [H]

Identifier: 148827310

GI number: 148827310

Start: 642301

End: 643341

Strand: Direct

Name: apbE [H]

Synonym: CGSHiGG_03455

Alternate gene names: 148827310

Gene position: 642301-643341 (Clockwise)

Preceding gene: 148827309

Following gene: 148827311

Centisome position: 34.03

GC content: 38.33

Gene sequence:

>1041_bases
ATGAAAAAATTAATAAGCGGTATCATAGCGGTAGCAATGGCATTAAGTCTTGCTGCTTGTCAAAAAGAAACAAAAGTTAT
CTCTTTAAGCGGTAAAACAATGGGGACAACTTATCATGTTAAATACCTTGATGATGGTTCAATAAAAGCAACATCTGAAA
AGACGCATGAAGAAATTGAAGCAATCTTAAAAGATGTGAATGCGAAAATGTCCACTTACAAAAAAGATTCGGAATTGAGC
CGCTTCAATCAAAATACCCAAGTGAACACACCGATTGAGATTTCAGCAGATTTTGCCAAAGTATTAGCTGAAGCGATTCG
TTTAAATAAAGTGACCGAAGGCGCGTTGGATGTAACTGTTGGTCCAGTTGTAAATTTATGGGGATTTGGTCCTGAAAAAC
GCCCCGAAAAACAACCTACACCAGAACAATTAGCTGAACGCCAAGCGTGGGTTGGCATTGATAAAATTGCCCTTGATATG
AGTGGTGCAAAACCGACATTAAGCAAAGCACTTCCTCAAGTTTACGTAGATTTATCGTCAATTGCTAAAGGCTTTGGCGT
TGATCAGGTGGCTGAAAAGTTAGAACAATTAAATGCTCAGAATTACATGGTTGAAATCGGCGGTGAAATTCGTGCGAAAG
GAAAAAATATTGAAGGTAAACCTTGGCAGATTGCCATTGAAAAACCAACTACAACAGGCGAAAGAGCGGTTGAAACTGTC
ATTGGATTAAACAATATGGGAATGGCAAGTTCTGGCGATTACCGTATTTACTTTGAAGAAAATGGCAAACGCTTTGCTCA
TGAAATTGATCCGAAAACAGGTTATCCAATTCAGCATCATTTAGCCTCAATTACGGTACTTGCACCAACCTCAATGACTG
CAGATGGCTTATCTACAGGGTTATTTGTGCTGGGGGAAGACAAGGCGTTAGAAGTGGCTGAGAAAAATAATCTTGCCGTT
TATTTAATCATTAGAACAGATAATGGTTTTGTTACCAAATCATCCTCTGCTTTCAAAAAATTAACAGAAACAAAAGAATA
G

Upstream 100 bases:

>100_bases
ATAAAGTGCGGTCAAAATTTAAGATGTTTTTAAAAATTTTCTATGATTACAGGTAAGTTTTGTTGAGTTTGCCAGTAATT
CACAAATTTAGGAAATCCAG

Downstream 100 bases:

>100_bases
TTATGCAAACTTTATTTTTTACTTTAATCGCTTTCGTCGCAATTATATTGTTGATGTCTATCGGCTTTATTATCAAAAAA
CAAAGTTTAAAAGGCAGCTG

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSIKATSEKTHEEIEAILKDVNAKMSTYKKDSELS
RFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKIALDM
SGAKPTLSKALPQVYVDLSSIAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVETV
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVAEKNNLAV
YLIIRTDNGFVTKSSSAFKKLTETKE

Sequences:

>Translated_346_residues
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSIKATSEKTHEEIEAILKDVNAKMSTYKKDSELS
RFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKIALDM
SGAKPTLSKALPQVYVDLSSIAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVETV
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVAEKNNLAV
YLIIRTDNGFVTKSSSAFKKLTETKE
>Mature_346_residues
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSIKATSEKTHEEIEAILKDVNAKMSTYKKDSELS
RFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKIALDM
SGAKPTLSKALPQVYVDLSSIAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVETV
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVAEKNNLAV
YLIIRTDNGFVTKSSSAFKKLTETKE

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=339, Percent_Identity=41.0029498525074, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37708; Mature: 37708

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSIKATSEKTHEEIE
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECEEEEEEEECCCCEEECCHHHHHHHH
AILKDVNAKMSTYKKDSELSRFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTV
HHHHHHCHHHHHHCCHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEC
GPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKIALDMSGAKPTLSKALPQVYVDLSS
CCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHH
IAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVETV
HHCCCCHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTG
HCCCCCCCCCCCCEEEEEECCCCEEHHCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCC
LFVLGEDKALEVAEKNNLAVYLIIRTDNGFVTKSSSAFKKLTETKE
EEEEECCCEEEEECCCCEEEEEEEECCCCEEECCHHHHHHHHHCCC
>Mature Secondary Structure
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSIKATSEKTHEEIE
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECEEEEEEEECCCCEEECCHHHHHHHH
AILKDVNAKMSTYKKDSELSRFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTV
HHHHHHCHHHHHHCCHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEC
GPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKIALDMSGAKPTLSKALPQVYVDLSS
CCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHH
IAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVETV
HHCCCCHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTG
HCCCCCCCCCCCCEEEEEECCCCEEHHCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCC
LFVLGEDKALEVAEKNNLAVYLIIRTDNGFVTKSSSAFKKLTETKE
EEEEECCCEEEEECCCCEEEEEEEECCCCEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]