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The map label for this gene is mrdA

Identifier: 148827188

GI number: 148827188

Start: 486226

End: 488181

Strand: Reverse

Name: mrdA

Synonym: CGSHiGG_02655

Alternate gene names: 148827188

Gene position: 488181-486226 (Counterclockwise)

Preceding gene: 148827189

Following gene: 148827187

Centisome position: 25.87

GC content: 38.55

Gene sequence:

>1956_bases
ATGAATTTAAAAAAATTCTTCAATACACCAACGCACGAACCGTTCCGCGATAAAAAAGCGGAGCGTAACCTTTTTGCACG
CCGAACGTTAGTGGCTTTTTTGGGGATTCTGCTCTTAACAGGCGTGTTATTCACCAATATTTATCAGCTACAGATTGTTA
ATTTCGATACTTACCAAACGCGTTCTAATGGTAACCGAATTAAATTATTACCTTTACCGCCAACACGTGGCTTAATCTAT
GATCGTTACGGCAAATTATTAGCCGAAAATCTTACTTTTTTTGGTTTATACATTGTGCCAGAAAAAACTGAAAATTTAGA
CCGCACTTTAGATGAATTACGCTATATTGTGGGATTGACGGATAACGATATTGAAAATTTTAAAAAGGAACGTCGCCGCG
GAACGCGTTATACACCGATTTTATTAAAGCCGAATTTAACAGAAGAACAAATTTCACGCTTTGCGGTAAATGGCTACCAA
TACCCGAGCTTAGAGGTACGTCCTTATTTCAAACGCCATTATTTATATGGCGAAACAATGGCGCATATTTTGGGCTATGT
AGGTAAAATGAATGATAAAGATGTAGAACGTTTGAAACGAGAGGATAAATTCGCGAATTATGCGGGAACGAACGATATTG
GAAAATTGGGTATTGAACGTTATTACGAGGATATACTGCAAGGTACCACGGGTTTTGAAGAAGTAGAAATTAATAATCGC
GGCAAAGTAATCCGCACTTTGCGTAGCCGACCTGCTGTGGCGGGAAAAAGTATTCATTTAACCATTGATTTGGCATTACA
GCGTTATATTACTGAACTTTTATCGGGACTAAAAGGTGCTGTAGTTGTGCTTGATCCGAAAGATAGTAGTGTATTAGCGA
TGGTATCTACACCAAGTTATGATAATAATTTATTTGTTGATGGTATTTCCTCCGAAGATTATAAACGTTTATTAAATGAT
CTTGCTCGTCCACTTTATAGTCGTGCGACACAAGGTGCGTATCCGCCCGCTTCGACAGTCAAACCTTTTATTGCAGTAGC
CGCACAAACTGAAAATGTGATTACACCAAACACAACGATTTTTGATCCGGGATATTGGGTATTACCTAATTCCACAAAAC
GTTTTCGTGATTGGAAAAAAACAGGCCACGGGGATACGGATTTGAATAAAGCGATTACTGAATCCTCTGATACTTATTTT
TATCAAGTGGCTTATAATATGGGTATTGATCGCCTTTCTAATTGGATGAAGGATTTTGGTTTTGGTATGCCAACGGGGAT
TGAAATTCAAGAAGAAACGGCTGCCAATATACCAACTCGAGAATGGAAACAAAAACGTTATAAACGCCCTTGGGTGCAAG
GCGATACGATTTCGGTTGGGATTGGTCAAGGTTATTGGACAGCAACACCACTACAAGTAGCAAAAGCGACGACAATATTG
GTCAATAATGGCAAAGTAAATACACCGCATTTGATGAAAGCAATTGAAGGTGCAGTATTAGAGCCTTATGAAGATCCGTT
ACTTTATCCTGATATTAACACACCTAAAGTGGCAGCTTGGGAGGCAGCGAAACGCGGTATGTACAATGTTGTTAATGCTG
CAAATGGAACTGGGCGAAAAGCCTTTGCAGATGCAAATTATCGTGTTGCAGGGAAATCTGGCACTGCACAAGTGTTTAGT
TTAAAGGAAAATGAAAAATATAATACGGCAGGCTTAAAAAAAGAATTGCATGATCACGCTTGGTTTACCGCTTACGCCCC
TTATGATAATCCTAAATTAGTTGTAACAGTGATTTTAGAAAATGCAGGTGGTGGTTCAAGTAATGCCGCACCGTTAGCAC
GTAAAGTGATGGATTACTATTTAAATCAGCGTTTACCACAAGTTGAAAAATATAATGTACCTATTCAGCAAAAAAAAGAT
TTGTCACAAGAGAGCGAACAAATAAATGGAAGATAA

Upstream 100 bases:

>100_bases
GCTTGTTCGTGTCGTGGTGGCTGAAAGTTTGTATCGTGCGTGGTCGCTAACCACTAATCATCCTTATCATCGAGAATAAT
ATATCCTTATTTTTAGTCAG

Downstream 100 bases:

>100_bases
AATCCCTTTTTGGTTGCGTGTTTGGCAACGTTTGCATATTGATTTTTGGCTGTTTATCGGACTTCTTGCAATAACTGCTT
ATGGTATGCTGGTGTTATAT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MNLKKFFNTPTHEPFRDKKAERNLFARRTLVAFLGILLLTGVLFTNIYQLQIVNFDTYQTRSNGNRIKLLPLPPTRGLIY
DRYGKLLAENLTFFGLYIVPEKTENLDRTLDELRYIVGLTDNDIENFKKERRRGTRYTPILLKPNLTEEQISRFAVNGYQ
YPSLEVRPYFKRHYLYGETMAHILGYVGKMNDKDVERLKREDKFANYAGTNDIGKLGIERYYEDILQGTTGFEEVEINNR
GKVIRTLRSRPAVAGKSIHLTIDLALQRYITELLSGLKGAVVVLDPKDSSVLAMVSTPSYDNNLFVDGISSEDYKRLLND
LARPLYSRATQGAYPPASTVKPFIAVAAQTENVITPNTTIFDPGYWVLPNSTKRFRDWKKTGHGDTDLNKAITESSDTYF
YQVAYNMGIDRLSNWMKDFGFGMPTGIEIQEETAANIPTREWKQKRYKRPWVQGDTISVGIGQGYWTATPLQVAKATTIL
VNNGKVNTPHLMKAIEGAVLEPYEDPLLYPDINTPKVAAWEAAKRGMYNVVNAANGTGRKAFADANYRVAGKSGTAQVFS
LKENEKYNTAGLKKELHDHAWFTAYAPYDNPKLVVTVILENAGGGSSNAAPLARKVMDYYLNQRLPQVEKYNVPIQQKKD
LSQESEQINGR

Sequences:

>Translated_651_residues
MNLKKFFNTPTHEPFRDKKAERNLFARRTLVAFLGILLLTGVLFTNIYQLQIVNFDTYQTRSNGNRIKLLPLPPTRGLIY
DRYGKLLAENLTFFGLYIVPEKTENLDRTLDELRYIVGLTDNDIENFKKERRRGTRYTPILLKPNLTEEQISRFAVNGYQ
YPSLEVRPYFKRHYLYGETMAHILGYVGKMNDKDVERLKREDKFANYAGTNDIGKLGIERYYEDILQGTTGFEEVEINNR
GKVIRTLRSRPAVAGKSIHLTIDLALQRYITELLSGLKGAVVVLDPKDSSVLAMVSTPSYDNNLFVDGISSEDYKRLLND
LARPLYSRATQGAYPPASTVKPFIAVAAQTENVITPNTTIFDPGYWVLPNSTKRFRDWKKTGHGDTDLNKAITESSDTYF
YQVAYNMGIDRLSNWMKDFGFGMPTGIEIQEETAANIPTREWKQKRYKRPWVQGDTISVGIGQGYWTATPLQVAKATTIL
VNNGKVNTPHLMKAIEGAVLEPYEDPLLYPDINTPKVAAWEAAKRGMYNVVNAANGTGRKAFADANYRVAGKSGTAQVFS
LKENEKYNTAGLKKELHDHAWFTAYAPYDNPKLVVTVILENAGGGSSNAAPLARKVMDYYLNQRLPQVEKYNVPIQQKKD
LSQESEQINGR
>Mature_651_residues
MNLKKFFNTPTHEPFRDKKAERNLFARRTLVAFLGILLLTGVLFTNIYQLQIVNFDTYQTRSNGNRIKLLPLPPTRGLIY
DRYGKLLAENLTFFGLYIVPEKTENLDRTLDELRYIVGLTDNDIENFKKERRRGTRYTPILLKPNLTEEQISRFAVNGYQ
YPSLEVRPYFKRHYLYGETMAHILGYVGKMNDKDVERLKREDKFANYAGTNDIGKLGIERYYEDILQGTTGFEEVEINNR
GKVIRTLRSRPAVAGKSIHLTIDLALQRYITELLSGLKGAVVVLDPKDSSVLAMVSTPSYDNNLFVDGISSEDYKRLLND
LARPLYSRATQGAYPPASTVKPFIAVAAQTENVITPNTTIFDPGYWVLPNSTKRFRDWKKTGHGDTDLNKAITESSDTYF
YQVAYNMGIDRLSNWMKDFGFGMPTGIEIQEETAANIPTREWKQKRYKRPWVQGDTISVGIGQGYWTATPLQVAKATTIL
VNNGKVNTPHLMKAIEGAVLEPYEDPLLYPDINTPKVAAWEAAKRGMYNVVNAANGTGRKAFADANYRVAGKSGTAQVFS
LKENEKYNTAGLKKELHDHAWFTAYAPYDNPKLVVTVILENAGGGSSNAAPLARKVMDYYLNQRLPQVEKYNVPIQQKKD
LSQESEQINGR

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786854, Length=608, Percent_Identity=59.046052631579, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=627, Percent_Identity=21.3716108452951, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBP2_HAEIN (P44469)

Other databases:

- EMBL:   L42023
- PIR:   C64044
- RefSeq:   NP_438205.1
- ProteinModelPortal:   P44469
- GeneID:   950929
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0032
- TIGR:   HI_0032
- HOGENOM:   HBG712588
- OMA:   WKKKTFK
- ProtClustDB:   CLSK870667
- BioCyc:   HINF71421:HI_0032-MONOMER
- DrugBank:   DB00303
- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790
- TIGRFAMs:   TIGR03423

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase; SSF56519 PBP_dimer; SSF56601 PBP_transp_fold

EC number: NA

Molecular weight: Translated: 73814; Mature: 73814

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: ACT_SITE 338-338

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKKFFNTPTHEPFRDKKAERNLFARRTLVAFLGILLLTGVLFTNIYQLQIVNFDTYQT
CCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCEEE
RSNGNRIKLLPLPPTRGLIYDRYGKLLAENLTFFGLYIVPEKTENLDRTLDELRYIVGLT
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCC
DNDIENFKKERRRGTRYTPILLKPNLTEEQISRFAVNGYQYPSLEVRPYFKRHYLYGETM
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AHILGYVGKMNDKDVERLKREDKFANYAGTNDIGKLGIERYYEDILQGTTGFEEVEINNR
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
GKVIRTLRSRPAVAGKSIHLTIDLALQRYITELLSGLKGAVVVLDPKDSSVLAMVSTPSY
CHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCC
DNNLFVDGISSEDYKRLLNDLARPLYSRATQGAYPPASTVKPFIAVAAQTENVITPNTTI
CCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHEEEECCCCEECCCCEE
FDPGYWVLPNSTKRFRDWKKTGHGDTDLNKAITESSDTYFYQVAYNMGIDRLSNWMKDFG
ECCCEEECCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHC
FGMPTGIEIQEETAANIPTREWKQKRYKRPWVQGDTISVGIGQGYWTATPLQVAKATTIL
CCCCCCCEECHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHEEEEEEE
VNNGKVNTPHLMKAIEGAVLEPYEDPLLYPDINTPKVAAWEAAKRGMYNVVNAANGTGRK
EECCCCCCHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHCCHHHHHCCCCCCCCE
AFADANYRVAGKSGTAQVFSLKENEKYNTAGLKKELHDHAWFTAYAPYDNPKLVVTVILE
EECCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEE
NAGGGSSNAAPLARKVMDYYLNQRLPQVEKYNVPIQQKKDLSQESEQINGR
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLKKFFNTPTHEPFRDKKAERNLFARRTLVAFLGILLLTGVLFTNIYQLQIVNFDTYQT
CCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCEEE
RSNGNRIKLLPLPPTRGLIYDRYGKLLAENLTFFGLYIVPEKTENLDRTLDELRYIVGLT
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCC
DNDIENFKKERRRGTRYTPILLKPNLTEEQISRFAVNGYQYPSLEVRPYFKRHYLYGETM
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AHILGYVGKMNDKDVERLKREDKFANYAGTNDIGKLGIERYYEDILQGTTGFEEVEINNR
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
GKVIRTLRSRPAVAGKSIHLTIDLALQRYITELLSGLKGAVVVLDPKDSSVLAMVSTPSY
CHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCC
DNNLFVDGISSEDYKRLLNDLARPLYSRATQGAYPPASTVKPFIAVAAQTENVITPNTTI
CCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHEEEECCCCEECCCCEE
FDPGYWVLPNSTKRFRDWKKTGHGDTDLNKAITESSDTYFYQVAYNMGIDRLSNWMKDFG
ECCCEEECCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHC
FGMPTGIEIQEETAANIPTREWKQKRYKRPWVQGDTISVGIGQGYWTATPLQVAKATTIL
CCCCCCCEECHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHEEEEEEE
VNNGKVNTPHLMKAIEGAVLEPYEDPLLYPDINTPKVAAWEAAKRGMYNVVNAANGTGRK
EECCCCCCHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHCCHHHHHCCCCCCCCE
AFADANYRVAGKSGTAQVFSLKENEKYNTAGLKKELHDHAWFTAYAPYDNPKLVVTVILE
EECCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEE
NAGGGSSNAAPLARKVMDYYLNQRLPQVEKYNVPIQQKKDLSQESEQINGR
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800