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The map label for this gene is spoT [H]

Identifier: 148827158

GI number: 148827158

Start: 449252

End: 451366

Strand: Reverse

Name: spoT [H]

Synonym: CGSHiGG_02450

Alternate gene names: 148827158

Gene position: 451366-449252 (Counterclockwise)

Preceding gene: 148827159

Following gene: 148827157

Centisome position: 23.92

GC content: 40.09

Gene sequence:

>2115_bases
TTGGAACTGTTTGCGGATTTAGATCGAATTATTCAAGGGTATTTGCCCGCAGATAAAATTGAACTCATCAAGCGTGCGTT
TGTGATTGCAAGAGATGCGCACGAAGGACAATTTCGCTCCAGCGGCGAACCTTACATTACCCATCCTGTGGCGGTAGCCT
CCATTATTGCTCAACTTCACTTAGATCACGAAGCGGTAATGGCTGCGCTTTTGCACGATGTTATTGAAGATACACCTTAT
ACGGAAGAACAACTGAAAGAAGAGTTCGGTGCCAGTGTGGCTGAAATTGTGGACGGTGTATCAAAACTCGATAAGTTGAA
ATTTCGTACTCGCCAAGAAGCACAAGTCGAGAATTTTCGCAAAATGATTTTGGCGATGACGCGCGATATTCGTGTTGTGT
TAATCAAGCTTGCTGACCGCACGCATAATATGCGCACGCTTGGTTCGTTACGACCAGATAAACGTCGCCGTATTGCGAAA
GAAACGCTTGAAATCTATTGTCCGCTTGCTCATCGTTTAGGTATTGAGCATATCAAAAATGAATTAGAAGACTTATCTTT
TCAAGCAATGCATCCACATCGTTATGAGGTATTAAAAAAACTCGTTGATGTGGCGCGGAGTAATCGACAAGATTTAATCG
AACGTATTTCACAAGAGATTAAAGTGCGGTTAGAAAACGCAGGCATTTTTGCCCGAGTGTGGGGACGTGAAAAACATCTT
TACAAGATCTATCAAAAAATGCGCATAAAGGATCAAGAATTCCACTCTATTATGGATATTTATGCGTTCCGTGTGATTGT
GAAAAATGTTGATGATTGTTATCGCGTTTTGGGGCAAATGCATAATCTTTATAAGCCACGTCCAGGCCGAGTTAAGGATT
ATATCGCCGTACCTAAAGCAAATGGCTATCAATCTTTGCAAACTTCAATGATTGGGCCGAAAGGAGTTCCCGTAGAAGTT
CATATCCATACTGAGGATATGGAACAAGTCGCTGAAATGGGCATTACTGCGCATTGGGTTTATAAAGAAAATGGTAAAAA
CGACAGTACAACCGCACAAATCCGCGTACAACGCTGGTTGCAAAGTTTGGTCGAAATTCAACAAAGTGTAGGTAATTCGT
TTGAGTTTATTGAGAATGTAAAATCAGAGTTTTTCCCGAAAGAAATTTATGTTTTTACGCCAAAAGGTCGAATTGTTGAA
TTACCAATGGACGCAACAGCAGTAGATTTCGCTTATGCTGTGCATTCAGATGTGGGGAATACTTGTGTTGGCGTAACCGT
TGAGCATAAACCTTATCCACTTTCAAAAGCCCTTGAATCAGGGCAAACAGTCAATATCATTACTGATCCAAATGCGCATC
CTGAAGTCGCATGGTTAAATTTTGTGGTAACGGCTCGTGCGAAAACACGGATTCGCCATTATCTCAAACAGCGTTGTGAA
GAGGATGCGGTGAAATTAGGCGAAGTTGAGCTTAATGTAGCATTACAGCCTCATAATTTAGGTGATTTTTCTATTCAACA
AATTAGCACTGTATTAGATGCTTTAGCCCTTTCTTCTTTAGATGAATTATTACGAGAAATTGGTTTAGGTAATCAGTCAG
CCTCCATGATTGCGCATCAATTTGTGGGCGTGCCGTTAGAATCTGCTAATACTAAGAATCTCGAATTTGAATCAAAAATA
CTCACTATTGCACCAATGCAAGTGGGTAAAACACAATTTGCACAATGTTGCCATCCAATTCTAGGTGATCCTATTGTGGG
ATGTTGTACAGAAAAAAATACAGTTGTGGTACACCATCAACATTGTGCAAGTTTGAAAAATGCTTGTCGTCAATCGTTGG
CTAAATGGGATAACGTTCAAAGTGCGGTGAATTTTGAGGCTGAATTACAAATAGAAATATTGAATGAGCAGAATGCCTTG
CTTAGTTTAATGACCGCAATTTCAGCTAGCGAAAGCAGTCTCCAAAATATTTGGACGGAAGAATTAGAGAACAATTTATT
GCTTGTGATTTTGCAGGTTTGTGTAAAAGATATCAAACACCTTGCCAATATCGTTCATCGTATTAAAGGCATCACTGGCG
TCGTTAATGTCAAACGCAATATTAATGAGCTATGA

Upstream 100 bases:

>100_bases
AGCAGCTGTTGCATTAATTACAGAATAATATTTTAAAAGTGCGGTAAAAAATCACGATATTTTAACTGCACTTTCTCTTT
CAACGATAAGTAGGTCTCCT

Downstream 100 bases:

>100_bases
GCCTTGAACTTCTCGATGCCGTGTCTTTGACCAGCCTTTCTGGTGTGGGGGCTGCCATTTCTAATAAACTGGCTAAAATC
GGCATTCATAACCTGCAAGA

Product: glutamate racemase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLHLDHEAVMAALLHDVIEDTPY
TEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAK
ETLEIYCPLAHRLGIEHIKNELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKANGYQSLQTSMIGPKGVPVEV
HIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWLQSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVE
LPMDATAVDFAYAVHSDVGNTCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQFVGVPLESANTKNLEFESKI
LTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQHCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNAL
LSLMTAISASESSLQNIWTEELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL

Sequences:

>Translated_704_residues
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLHLDHEAVMAALLHDVIEDTPY
TEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAK
ETLEIYCPLAHRLGIEHIKNELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKANGYQSLQTSMIGPKGVPVEV
HIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWLQSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVE
LPMDATAVDFAYAVHSDVGNTCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQFVGVPLESANTKNLEFESKI
LTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQHCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNAL
LSLMTAISASESSLQNIWTEELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL
>Mature_704_residues
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLHLDHEAVMAALLHDVIEDTPY
TEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAK
ETLEIYCPLAHRLGIEHIKNELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKANGYQSLQTSMIGPKGVPVEV
HIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWLQSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVE
LPMDATAVDFAYAVHSDVGNTCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQFVGVPLESANTKNLEFESKI
LTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQHCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNAL
LSLMTAISASESSLQNIWTEELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=711, Percent_Identity=57.1026722925457, Blast_Score=833, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=686, Percent_Identity=31.3411078717201, Blast_Score=348, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI25143118, Length=142, Percent_Identity=33.8028169014084, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79830; Mature: 79830

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLH
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAKETLEIYCPLAHRLGIEHIKN
HHHHHHHCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHH
ELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEHHCCCEEEEECCCHHHH
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLQTSMIGPKGVPVEVHIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWL
CCHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHH
QSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVELPMDATAVDFAYAVHSDVGN
HHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCC
TCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
CEEEEEEECCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQ
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FVGVPLESANTKNLEFESKILTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQ
HHCCCCCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEHH
HCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNALLSLMTAISASESSLQNIWTE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHH
ELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCC
>Mature Secondary Structure
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLH
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAKETLEIYCPLAHRLGIEHIKN
HHHHHHHCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHH
ELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEHHCCCEEEEECCCHHHH
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLQTSMIGPKGVPVEVHIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWL
CCHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHH
QSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVELPMDATAVDFAYAVHSDVGN
HHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCC
TCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
CEEEEEEECCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQ
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FVGVPLESANTKNLEFESKILTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQ
HHCCCCCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEHH
HCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNALLSLMTAISASESSLQNIWTE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHH
ELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]