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The map label for this gene is metG [H]

Identifier: 148827028

GI number: 148827028

Start: 274296

End: 276344

Strand: Direct

Name: metG [H]

Synonym: CGSHiGG_01600

Alternate gene names: 148827028

Gene position: 274296-276344 (Clockwise)

Preceding gene: 148827026

Following gene: 148827033

Centisome position: 14.53

GC content: 40.26

Gene sequence:

>2049_bases
ATGACAACTCAACCCCGTAAAATTTTAGTCACTTGCGCCTTGCCTTATGCCAATGGGGCAATTCATTTAGGCCATATGTT
AGAACATATCCAAGCGGATATTTGGGTGCGTTTTCAACGTATGCGTGGCAATAAAATCCATTTTGTCTGTGCGGACGATG
CACATGGCACACCGATTATGCTGAATGCCGATAAACTTGGCATTACACCTGAAGAATTAATTGCTAAAGCAAAAGCCGAT
CACATTCGTGATTTTGCAGGTTTCAATATTAGTTTTGATAACTATCATTCCACTCACAGCGAAGAAAACAAACAGCTCAC
TGCAGAAATTTACAATAAACTCAAAGCCAATGGCTTTATTAAGAGTAAAGTTATTTCTCAGTTATTCGACCCTGAAAAAA
ATATGTTCTTGCCCGATCGTTTTGTGAAAGGCACTTGCCCGAAATGTAAAGCAGAAGATCAATATGGCGATAACTGCGAA
GTTTGTGCTTCTACTTACAGCCCAATGGATTTAATTAATCCTCGTTCAGCTGTTTCTGGCACAACACCTATCGTAAAAGA
ATCTGAACACTTTTTCTTTGACTTACCTGCATTTGAAGGGATGTTAAAAGAATGGACACGTTCTGGCTCACTTCAATCTG
AAATTGCCAACAAAATGCAAGAATGGTTTGAGAGCGGTTTACAACAATGGGATATTTCACGCGATGCGCCTTATTTCGGT
TTTGAAATTCCGGGCGCAAAAGATAAATTCTTTTATGTTTGGTTAGATGCGCCAATCGGCTATATGGCTTCATTCAAAAA
TCTATGCAATCGTGAAGGCATTGATTTCAATGAATTTTGGGCGGAAGGCAGTGATGCGGAGCTTTATCATTTCATCGGTA
AAGATATCGTTTATTTTCACAGTCTATTTTGGCCTGCAATGCTTGAAGGCAGTGGCTATCGTAAACCGACTAATGTGTTC
GCTCATGGCTATGTCACGGTGGATGGGGCGAAGATGTCAAAATCTCGCGGGACTTTCATTCAAGCCAGCACTTATTTGAA
TCATATCGATCCTGAATGTTTGCGTTATTACTATGCAGCAAAATTAAATGATCGCATTGAAGATTTAGACTTTAACCTTG
ACGATTTCGTACAACGTGTAAATACGGATATTGTAAACAAATTAGTGAATTTAGCTTCACGCAATGCAGGCTTTATTGCG
AAACGCTTTGAAAGCAAACTTGCGGATAAACTGGAAGACGAATCATTATTTGCAGAATTTACCGCCCAAGCCGAGCAAAT
CGCCGCTTATTACGAAAGCCGTGAATACAATAAAGCCATTCGTGAAATTATGGCATTAACGGATAAAGCCAATAAATACA
TTGATGAAAAAGCCCCTTGGGTGATTGCAAAAGAAGAAGGCAAAGAGGCGGAATTGCAAGCCGTATGTTCAATGGGTATT
GAGCTTTTCCGCGTGTTGATGTCTTACTTAAAACCTGTTCTTCCAAAACTGGCAGAACGTGCCGAAGCCTTCTTACAAGC
TGAATTACGTTGGGATAATATTCATCAACCATTGCTTGGTCATACCCTTGCACCGTTCAAAGCCTTATTCTCTCGTTTAG
AGAAAAAACAAATTGATGCCGTTGTGGAAGAAACCAAAGCCTTATTTGCAGCAGCCAATAAAGCCGCAGAAAAAACAGAA
GCAAAACCAACCGCACTTTCTGCCGTTGAACCTATTGCTGAAACCATCACGATTGATGATTTCGCTAAACTTGATATGCG
TGTAGCAAAAGTGTTGAAATGCGAAGCAGTGCCAGAATCTAATAAACTTTTACGTTTTGAATTGGATCTTGGCGATCATA
CTCGTCAAGTCTTCTCTGGCATTAAAGCGGCTTACAATAAACCTGAAGAATTAGAAGGTCGTTTTGTGATTATGGTGGCA
AACCTTGCACCACGTAAAATGAAATTCGGCGTGTCTGAGGGAATGATTCTATCCGCAGGAACAGGCGGTAGTGATTTATT
CTTGTTATCTGCCGATAATGGTGTCACCGCAGGTATGCAAGTAAAATAA

Upstream 100 bases:

>100_bases
AAAGTGCGGTATGTTAAGTTAAAGTTTTTCAAACTAGAAAAAATCCCCTATTTAAAGTAAGATAAGCACAATTTTTGTAT
GCTAAAAAAGAGAATAATAA

Downstream 100 bases:

>100_bases
TCAATTCAATTAATTAGGGCAACATTACGTTGCCCTTTTATTTTCTTGTTGAAAATAGATGACTTATAACCTACCATAAC
ACTCAACTATTTATCTGTTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MTTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIMLNADKLGITPEELIAKAKAD
HIRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
VCASTYSPMDLINPRSAVSGTTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFG
FEIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVF
AHGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNTDIVNKLVNLASRNAGFIA
KRFESKLADKLEDESLFAEFTAQAEQIAAYYESREYNKAIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGI
ELFRVLMSYLKPVLPKLAERAEAFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDAVVEETKALFAAANKAAEKTE
AKPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPESNKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVA
NLAPRKMKFGVSEGMILSAGTGGSDLFLLSADNGVTAGMQVK

Sequences:

>Translated_682_residues
MTTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIMLNADKLGITPEELIAKAKAD
HIRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
VCASTYSPMDLINPRSAVSGTTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFG
FEIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVF
AHGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNTDIVNKLVNLASRNAGFIA
KRFESKLADKLEDESLFAEFTAQAEQIAAYYESREYNKAIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGI
ELFRVLMSYLKPVLPKLAERAEAFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDAVVEETKALFAAANKAAEKTE
AKPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPESNKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVA
NLAPRKMKFGVSEGMILSAGTGGSDLFLLSADNGVTAGMQVK
>Mature_681_residues
TTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIMLNADKLGITPEELIAKAKADH
IRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCEV
CASTYSPMDLINPRSAVSGTTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFGF
EIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFA
HGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNTDIVNKLVNLASRNAGFIAK
RFESKLADKLEDESLFAEFTAQAEQIAAYYESREYNKAIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGIE
LFRVLMSYLKPVLPKLAERAEAFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDAVVEETKALFAAANKAAEKTEA
KPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPESNKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVAN
LAPRKMKFGVSEGMILSAGTGGSDLFLLSADNGVTAGMQVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=635, Percent_Identity=28.8188976377953, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI24308436, Length=515, Percent_Identity=25.8252427184466, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1788432, Length=684, Percent_Identity=68.4210526315789, Blast_Score=1015, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=35.7798165137615, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17540794, Length=585, Percent_Identity=29.5726495726496, Blast_Score=252, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI212645096, Length=512, Percent_Identity=25.390625, Blast_Score=156, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71992781, Length=408, Percent_Identity=26.2254901960784, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321704, Length=554, Percent_Identity=29.2418772563177, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321610, Length=547, Percent_Identity=22.1206581352834, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24655311, Length=555, Percent_Identity=29.3693693693694, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI21358483, Length=515, Percent_Identity=24.8543689320388, Blast_Score=143, Evalue=4e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76950; Mature: 76818

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIM
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LNADKLGITPEELIAKAKADHIRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFI
EECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCH
KSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCEVCASTYSPMDLINPRSAVSG
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCCHHCCC
TTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFG
CCCCEECCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FEIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFH
EECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
SLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAA
HHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHH
KLNDRIEDLDFNLDDFVQRVNTDIVNKLVNLASRNAGFIAKRFESKLADKLEDESLFAEF
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TAQAEQIAAYYESREYNKAIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
ELFRVLMSYLKPVLPKLAERAEAFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHH
VVEETKALFAAANKAAEKTEAKPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPES
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
NKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVANLAPRKMKFGVSEGMILSAG
CCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCHHHCCCCCCEEEEEC
TGGSDLFLLSADNGVTAGMQVK
CCCCEEEEEECCCCCCCCCEEC
>Mature Secondary Structure 
TTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIM
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LNADKLGITPEELIAKAKADHIRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFI
EECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCH
KSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCEVCASTYSPMDLINPRSAVSG
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCCHHCCC
TTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFG
CCCCEECCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FEIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFH
EECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
SLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAA
HHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHH
KLNDRIEDLDFNLDDFVQRVNTDIVNKLVNLASRNAGFIAKRFESKLADKLEDESLFAEF
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TAQAEQIAAYYESREYNKAIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
ELFRVLMSYLKPVLPKLAERAEAFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHH
VVEETKALFAAANKAAEKTEAKPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPES
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
NKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVANLAPRKMKFGVSEGMILSAG
CCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCHHHCCCCCCEEEEEC
TGGSDLFLLSADNGVTAGMQVK
CCCCEEEEEECCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA