Click here to switch to the map view.

The map label for this gene is haeIIM [H]

Identifier: 148826843

GI number: 148826843

Start: 104695

End: 105651

Strand: Direct

Name: haeIIM [H]

Synonym: CGSHiGG_00545

Alternate gene names: 148826843

Gene position: 104695-105651 (Clockwise)

Preceding gene: 148826842

Following gene: 148826844

Centisome position: 5.55

GC content: 30.93

Gene sequence:

>957_bases
ATGAAAGAATATAAAACAATAGATTTGTTTGCTGGAATTGGTGGCATTCGTCTAGGTTTTGAAGCATTTGGATGTAAAAA
CGTATTTTCATCAGAATGGGATAAGTATGCTCAAAGTATGTATGAAGTAAATTTTGGCGAAAAACCTTTTGGAGATATTA
ATGATATATCTCCTAGCGATATACCAGATCATGATATTTTACTTGCTGGATTTCCTTGCCAACCATTTAGTATTGCTGGT
AAAGGATTGGGCTTTGCTGATACTAGAGGAACATTATTTTTTAATATAGAAGCAATTTTGAAAGCTAAAAAACCAAAAGC
TTTTTTGCTGGAAAATGTAAAAAGATTAACAACACATGATAATGGTAATACATTTAAAGTTATTAACGATAAATTAAATA
AGTTAGGATATACGGTATATCATAAAGTATTAAATACCCTTGATTTTGGATTACCACAAAAAAGAGAGCGAATTTACATT
GTGGGTTTTTTAGATAAATTGCATTTTGAATTTCCAAAGCCTATAGGAAAGTATGAAGAACTTTCTAATATATTAGAGAG
TGATGATGTTGTTCCTAATAATTATTTTCTTTCAGATGAACTAAAACGGAAAAGATTAAGTTCAATTACTAAAGATGTGC
CTTATCCTTCAATTTGGCATGAAAATATTTCAGGAAATGTTTCTGCTCTTCCATACTCTTGTGCATTAAGAGCTGGGGGA
AGTTATAATTATCTAGTTGTAAATGGGGTACGTCGATTAACTGGGCGAGAAATGCTAAGATTGCAAGGATTTCCAGATAC
TTTTGAAATTAATATACCTTATTCTCAGGTACGTAAAGTTGCTGGCAATTCAGTTAGTGTTCCTGTAATTAGGGCTATTG
CTGGTTCAATGATTAATAGTTTAAATATGGCAATGCCTAAAATAAATCAAGCAATTCAACTTTCATTATTGGACTAA

Upstream 100 bases:

>100_bases
TGATGATTTATCGGGTATTTATCCAGATTGGCAAGCAATTAATAAACGTGGCGCAGAAGTGCAAGCGCATGTGATTGATA
AGTATTAATTAGTAATAAGT

Downstream 100 bases:

>100_bases
AAATGAACGATATTCAAGTAGCAAAAGAATCTTTAGATAAAATTATAAAGAAAGCAAGAGTACATTTATACAAGCCTATA
CAAATTGCTGAAATTCTTTA

Product: modification methylase HaeII

Products: NA

Alternate protein names: M.HaeII; Cytosine-specific methyltransferase HaeII [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKEYKTIDLFAGIGGIRLGFEAFGCKNVFSSEWDKYAQSMYEVNFGEKPFGDINDISPSDIPDHDILLAGFPCQPFSIAG
KGLGFADTRGTLFFNIEAILKAKKPKAFLLENVKRLTTHDNGNTFKVINDKLNKLGYTVYHKVLNTLDFGLPQKRERIYI
VGFLDKLHFEFPKPIGKYEELSNILESDDVVPNNYFLSDELKRKRLSSITKDVPYPSIWHENISGNVSALPYSCALRAGG
SYNYLVVNGVRRLTGREMLRLQGFPDTFEINIPYSQVRKVAGNSVSVPVIRAIAGSMINSLNMAMPKINQAIQLSLLD

Sequences:

>Translated_318_residues
MKEYKTIDLFAGIGGIRLGFEAFGCKNVFSSEWDKYAQSMYEVNFGEKPFGDINDISPSDIPDHDILLAGFPCQPFSIAG
KGLGFADTRGTLFFNIEAILKAKKPKAFLLENVKRLTTHDNGNTFKVINDKLNKLGYTVYHKVLNTLDFGLPQKRERIYI
VGFLDKLHFEFPKPIGKYEELSNILESDDVVPNNYFLSDELKRKRLSSITKDVPYPSIWHENISGNVSALPYSCALRAGG
SYNYLVVNGVRRLTGREMLRLQGFPDTFEINIPYSQVRKVAGNSVSVPVIRAIAGSMINSLNMAMPKINQAIQLSLLD
>Mature_318_residues
MKEYKTIDLFAGIGGIRLGFEAFGCKNVFSSEWDKYAQSMYEVNFGEKPFGDINDISPSDIPDHDILLAGFPCQPFSIAG
KGLGFADTRGTLFFNIEAILKAKKPKAFLLENVKRLTTHDNGNTFKVINDKLNKLGYTVYHKVLNTLDFGLPQKRERIYI
VGFLDKLHFEFPKPIGKYEELSNILESDDVVPNNYFLSDELKRKRLSSITKDVPYPSIWHENISGNVSALPYSCALRAGG
SYNYLVVNGVRRLTGREMLRLQGFPDTFEINIPYSQVRKVAGNSVSVPVIRAIAGSMINSLNMAMPKINQAIQLSLLD

Specific function: This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HaeII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=384, Percent_Identity=34.1145833333333, Blast_Score=158, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 35692; Mature: 35692

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEYKTIDLFAGIGGIRLGFEAFGCKNVFSSEWDKYAQSMYEVNFGEKPFGDINDISPSD
CCCCEEEEHHHCCCCCEECHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
IPDHDILLAGFPCQPFSIAGKGLGFADTRGTLFFNIEAILKAKKPKAFLLENVKRLTTHD
CCCCCEEEECCCCCCHHCCCCCCCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHCCC
NGNTFKVINDKLNKLGYTVYHKVLNTLDFGLPQKRERIYIVGFLDKLHFEFPKPIGKYEE
CCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCCCCHHHH
LSNILESDDVVPNNYFLSDELKRKRLSSITKDVPYPSIWHENISGNVSALPYSCALRAGG
HHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEECCC
SYNYLVVNGVRRLTGREMLRLQGFPDTFEINIPYSQVRKVAGNSVSVPVIRAIAGSMINS
CCCEEEECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHH
LNMAMPKINQAIQLSLLD
HHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MKEYKTIDLFAGIGGIRLGFEAFGCKNVFSSEWDKYAQSMYEVNFGEKPFGDINDISPSD
CCCCEEEEHHHCCCCCEECHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
IPDHDILLAGFPCQPFSIAGKGLGFADTRGTLFFNIEAILKAKKPKAFLLENVKRLTTHD
CCCCCEEEECCCCCCHHCCCCCCCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHCCC
NGNTFKVINDKLNKLGYTVYHKVLNTLDFGLPQKRERIYIVGFLDKLHFEFPKPIGKYEE
CCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCCCCHHHH
LSNILESDDVVPNNYFLSDELKRKRLSSITKDVPYPSIWHENISGNVSALPYSCALRAGG
HHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEECCC
SYNYLVVNGVRRLTGREMLRLQGFPDTFEINIPYSQVRKVAGNSVSVPVIRAIAGSMINS
CCCEEEECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHH
LNMAMPKINQAIQLSLLD
HHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9628358 [H]