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The map label for this gene is bioD [H]

Identifier: 148826794

GI number: 148826794

Start: 31372

End: 32013

Strand: Direct

Name: bioD [H]

Synonym: CGSHiGG_00205

Alternate gene names: 148826794

Gene position: 31372-32013 (Clockwise)

Preceding gene: 148826793

Following gene: 148826795

Centisome position: 1.66

GC content: 34.74

Gene sequence:

>642_bases
ATGGGAAAGGTTATTTTTATATCTGGCATTGATACTGATGTGGGAAAAACGATTGCAACAGGCATTTATGCTAAAAAGTT
GATGGAACAAGGCTGTTCCGTGATTACACAAAAAATGATTCAAACAGGCTGTAAAAATATTGCTGATGATTTGCTTGTAC
ATCGAAAGATTCAAGGGATTGATTTAACGGAAGAAGATTTACAAGGCAAAACCTGCCCTTATGTTTTTGAATATCCTTGT
TCGCCACATTTAGCTGCAAAGCGAGAAAGTAGAAAAATTGCAGCAAAAATCATTGAAAAATCTACCGCACTTTTGGCAGA
AAAATATGATTATGTACTATTGGAGGGTGCTGGTGGCTTAATGGTACCTTATTGTGAAGAGGCGACGACGTTGGATTATA
TCCAGTTAAATAATTACCCTTTAATTTTAGTGACATCGGGAAAACTAGGCAGTATAAACCACACATTATTAAGTTTAGAG
GCTTGTCGTACTCGAGGTATTTCAGTATTAAGTGTAATGTATAACGGTTACCCAGAATATGATCCTATTATTAGTCAAGA
AACCCAACGCTATTTAAAACGTTATCTTGAAAAACATTTTCCTGAAACAGGATTTGAATACTTTAGGTGTATTGAAATTT
AA

Upstream 100 bases:

>100_bases
GTCGGCGTTCAGTATGCCGTCGGGCAAAGTGCGTCTGACTTATCATCCGTTATTTTTTATCGCGCGCTATTCTCACATAG
AAAATCAGTAAGGAATATTT

Downstream 100 bases:

>100_bases
GCTCAAGGAAAAGTGTGATAGACTCGTGCAAGTTTTTGATGGTGTTATCTGCCGCTAAAGTGCGGTTAAAACGAGTAAAA
AATGAATAACTATTTATTAA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase 2; DTBS 2; Dethiobiotin synthase 2 [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MGKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGIDLTEEDLQGKTCPYVFEYPC
SPHLAAKRESRKIAAKIIEKSTALLAEKYDYVLLEGAGGLMVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLE
ACRTRGISVLSVMYNGYPEYDPIISQETQRYLKRYLEKHFPETGFEYFRCIEI

Sequences:

>Translated_213_residues
MGKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGIDLTEEDLQGKTCPYVFEYPC
SPHLAAKRESRKIAAKIIEKSTALLAEKYDYVLLEGAGGLMVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLE
ACRTRGISVLSVMYNGYPEYDPIISQETQRYLKRYLEKHFPETGFEYFRCIEI
>Mature_212_residues
GKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGIDLTEEDLQGKTCPYVFEYPCS
PHLAAKRESRKIAAKIIEKSTALLAEKYDYVLLEGAGGLMVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLEA
CRTRGISVLSVMYNGYPEYDPIISQETQRYLKRYLEKHFPETGFEYFRCIEI

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=179, Percent_Identity=30.1675977653631, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081944, Length=195, Percent_Identity=28.2051282051282, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 23966; Mature: 23835

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGI
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLTEEDLQGKTCPYVFEYPCSPHLAAKRESRKIAAKIIEKSTALLAEKYDYVLLEGAGGL
CCCHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
MVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLEACRTRGISVLSVMYNGYPEY
EEECCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCC
DPIISQETQRYLKRYLEKHFPETGFEYFRCIEI
CCCCCHHHHHHHHHHHHHHCCCCCHHHEEEECC
>Mature Secondary Structure 
GKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGI
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLTEEDLQGKTCPYVFEYPCSPHLAAKRESRKIAAKIIEKSTALLAEKYDYVLLEGAGGL
CCCHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
MVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLEACRTRGISVLSVMYNGYPEY
EEECCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCC
DPIISQETQRYLKRYLEKHFPETGFEYFRCIEI
CCCCCHHHHHHHHHHHHHHCCCCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]