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The map label for this gene is rep [H]

Identifier: 148826716

GI number: 148826716

Start: 1766240

End: 1768255

Strand: Direct

Name: rep [H]

Synonym: CGSHiEE_08955

Alternate gene names: 148826716

Gene position: 1766240-1768255 (Clockwise)

Preceding gene: 148826715

Following gene: 148826718

Centisome position: 97.42

GC content: 40.62

Gene sequence:

>2016_bases
ATGAAACTCAATCCTCAACAACAACAAGCCGTTGAATATGTGACAGGCCCTTGTCTTGTGCTTGCTGGCGCTGGCTCAGG
CAAAACTCGTGTAATCATTAATAAAATCGCCTATTTAATTGAAAAGTGCGGTTATTCTCCGAAACAAATTGCCGCAGTGA
CTTTCACTAACAAAGCCGCACGCGAGATGAAGGAGCGTGTAGCACATTCCATTGGAAAAGAACAATCTAAAGGTTTGCTC
GTTTCCACTTTTCATACCCTCGGTTTTGACATTATTAAGCGTGAATATAAAGCGTTGGGCTTTAAATCAAATATGACCTT
GTTTGATGAACAGGATCAATTTGCGTTGTTAAAAGAGCTAACCGCTGATGTGTTAAAAGAAGATAAGGATTTATTGCGTG
AGTTAATTTCAGTGATTTCTAACTGGAAGAATGATTTGATTTCGCCAAAACAGGCATTTGCGTTGGCGCGTGATGCAAAA
TATCAAACTTTTGCAAAATGTTATGAGCGTTACGCCACACAAATTCGAGCTTACAATGCCCTTGATTTTGATGATTTGAT
TATGCTGCCGACATTGTTGTTCAAGCAAAATGAAGAAGTGCGGGCAAAATGGCAGGCAAAAATTCGTTATTTGTTGGTGG
ATGAATATCAAGATACCAACACCAGCCAATATGAACTGATTAAACTTTTAGTGGGGGATCGTGCTTGTTTTACTGTGGTG
GGCGATGATGACCAATCTATTTATTCGTGGCGTGGCGCACGACCAGAAAATATGGTGCGTTTACGCGATGATTTCCCTCG
TTTGAACGTGATTAAGCTAGAGCAAAATTATCGTTCAACCCAACGCATTCTGCATTGTGCCAATATTTTGATTGATAACA
ATGAGCACGTGTTTGATAAGAAACTCTTTTCAACCATTGGCGAAGGGGAAAAATTACTTATTATCGAAGCAAAAAATGAA
GAACACGAAGCAGAGCGGATTGTCGCTGAGTTGATCGCCCATCGTTTTAGCCGTAAAACCAAATATAAAGATTATGCGAT
TTTGTATCGAGGCAATCATCAATCTCGATTACTCGAAAAAGTACTGATGCAAAACCGTATTCCTTACAAAATTTCGGGTG
GTACTTCTTTTTTCTCCCGTGCAGAAATCAAAGATATGATGGCGTACTTGCGCTTGGTGGTGAATCAAGATGATGATGCC
GCATTCCTGCGTATTGTGAATACACCGAAACGTGAAATTGGCACCGCAACCTTACAAAAACTTGGCGAGTTGGCTCAAGA
AAAACACATCAGTTTATTTGATGCTATTTTTGAGTTTGAACTTATTCAACGCATCACGCCAAAAGCCTATGATTCATTGC
AAAAATTTGGCCGTTGGATTGTAGAACTTAATGATGAAATTCAACGTTCTGAATCAGAACGTGCGGTACGTTCAATGTTA
TCTGTGATTCATTATGAAGAATATTTGTACGAATACGCAACAAGCCCTAAAGCCGCAGAAATGCAAAGTAAGAATGTCGC
CACGTTATTTGACTGGGTTGCGGATATGTTAAAAGGCGATGAAACCAATGAGCCGATGAACCTTAATCAAGTTGTAACCC
GCCTGACATTACGGGATATGTTGGAGCGAGGCGAAGACGATGACGACAGCGATCAAGTTCAACTGATGACATTGCACGCC
TCTAAGGGATTAGAATTTCCTTATGTTTATTTGATCGGTATGGAAGAGGGCATTTTGCCCCACCAAACTAGCATTGATGA
AGACAACGTGGAAGAAGAACGCCGCTTGGCTTATGTTGGCATCACAAGAGCGCAAAAAGAGCTCATTTTTTCCTTGTGTC
GAGAACGCCGTCAATATGGAGAATTAGTTCGTCCAGAACCCAGCAGATTTTTAGCTGAATTACCCAATGACGATGTGTTA
TGGGAACGTGATAAACCAAAACTAACCACCGAGCAAAAACAAGAAAAAACACAAAACCAACTTGATAGATTGAGGGCGAT
TTTGAAAGGAGACTAG

Upstream 100 bases:

>100_bases
TTGAAATGGCCCAACAATGGGGATTGCCAATCTTAGATTTGCCTCTCTCATTTTTATTAGACACCGTATTACTGCCCTAT
GCGTGGGCCCAATAATTCTT

Downstream 100 bases:

>100_bases
AAAATCTAGCTCGGAATGAAAAGACAAGAGGGGAAAAGTGCGGTTAGGTTTTAAAGTATTTTTACAAATGGACTTTAAAA
TTTGATGCTTATGAATTTTA

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLIEKCGYSPKQIAAVTFTNKAAREMKERVAHSIGKEQSKGLL
VSTFHTLGFDIIKREYKALGFKSNMTLFDEQDQFALLKELTADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAK
YQTFAKCYERYATQIRAYNALDFDDLIMLPTLLFKQNEEVRAKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDKKLFSTIGEGEKLLIIEAKNE
EHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEKVLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDA
AFLRIVNTPKREIGTATLQKLGELAQEKHISLFDAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSESERAVRSML
SVIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDMLERGEDDDDSDQVQLMTLHA
SKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVGITRAQKELIFSLCRERRQYGELVRPEPSRFLAELPNDDVL
WERDKPKLTTEQKQEKTQNQLDRLRAILKGD

Sequences:

>Translated_671_residues
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLIEKCGYSPKQIAAVTFTNKAAREMKERVAHSIGKEQSKGLL
VSTFHTLGFDIIKREYKALGFKSNMTLFDEQDQFALLKELTADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAK
YQTFAKCYERYATQIRAYNALDFDDLIMLPTLLFKQNEEVRAKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDKKLFSTIGEGEKLLIIEAKNE
EHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEKVLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDA
AFLRIVNTPKREIGTATLQKLGELAQEKHISLFDAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSESERAVRSML
SVIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDMLERGEDDDDSDQVQLMTLHA
SKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVGITRAQKELIFSLCRERRQYGELVRPEPSRFLAELPNDDVL
WERDKPKLTTEQKQEKTQNQLDRLRAILKGD
>Mature_671_residues
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLIEKCGYSPKQIAAVTFTNKAAREMKERVAHSIGKEQSKGLL
VSTFHTLGFDIIKREYKALGFKSNMTLFDEQDQFALLKELTADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAK
YQTFAKCYERYATQIRAYNALDFDDLIMLPTLLFKQNEEVRAKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDKKLFSTIGEGEKLLIIEAKNE
EHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEKVLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDA
AFLRIVNTPKREIGTATLQKLGELAQEKHISLFDAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSESERAVRSML
SVIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDMLERGEDDDDSDQVQLMTLHA
SKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVGITRAQKELIFSLCRERRQYGELVRPEPSRFLAELPNDDVL
WERDKPKLTTEQKQEKTQNQLDRLRAILKGD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=668, Percent_Identity=66.9161676646707, Blast_Score=931, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=643, Percent_Identity=39.0357698289269, Blast_Score=417, Evalue=1e-117,
Organism=Escherichia coli, GI1787196, Length=352, Percent_Identity=27.8409090909091, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=457, Percent_Identity=31.0722100656455, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324477, Length=682, Percent_Identity=22.8739002932551, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77834; Mature: 77834

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLIEKCGYSPKQIAAVTFTNKAA
CCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCHHH
REMKERVAHSIGKEQSKGLLVSTFHTLGFDIIKREYKALGFKSNMTLFDEQDQFALLKEL
HHHHHHHHHHHCCHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHH
TADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAKYQTFAKCYERYATQIRAYNA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
LDFDDLIMLPTLLFKQNEEVRAKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDK
CCCCCHHHHCCCCCCCCCEEHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHH
KLFSTIGEGEKLLIIEAKNEEHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEK
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
VLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDAAFLRIVNTPKREIGTATLQK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
LGELAQEKHISLFDAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSESERAVRSML
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SVIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDM
HHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LERGEDDDDSDQVQLMTLHASKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVG
HHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
ITRAQKELIFSLCRERRQYGELVRPEPSRFLAELPNDDVLWERDKPKLTTEQKQEKTQNQ
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHH
LDRLRAILKGD
HHHHHHHHCCC
>Mature Secondary Structure
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLIEKCGYSPKQIAAVTFTNKAA
CCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCHHH
REMKERVAHSIGKEQSKGLLVSTFHTLGFDIIKREYKALGFKSNMTLFDEQDQFALLKEL
HHHHHHHHHHHCCHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHH
TADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAKYQTFAKCYERYATQIRAYNA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
LDFDDLIMLPTLLFKQNEEVRAKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDK
CCCCCHHHHCCCCCCCCCEEHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHH
KLFSTIGEGEKLLIIEAKNEEHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEK
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
VLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDAAFLRIVNTPKREIGTATLQK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
LGELAQEKHISLFDAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSESERAVRSML
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SVIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDM
HHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LERGEDDDDSDQVQLMTLHASKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVG
HHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
ITRAQKELIFSLCRERRQYGELVRPEPSRFLAELPNDDVLWERDKPKLTTEQKQEKTQNQ
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHH
LDRLRAILKGD
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]