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The map label for this gene is nrfC [H]

Identifier: 148826320

GI number: 148826320

Start: 1336223

End: 1336900

Strand: Direct

Name: nrfC [H]

Synonym: CGSHiEE_06745

Alternate gene names: 148826320

Gene position: 1336223-1336900 (Clockwise)

Preceding gene: 148826319

Following gene: 148826322

Centisome position: 73.7

GC content: 41.45

Gene sequence:

>678_bases
ATGACAGTCTGCTCACGCCGAAACTTTGTTTCTGGCATGGGGGCAGTGATCCTTATGACGGGAACATCTTTACCTTCTTT
TGCAAAAGAAGACAAGGCGGATAAACCTAAACGTTACGCAATGATACATGATGAAAATGCGTGTATCGGTTGTACAGCCT
GTATGGACGCATGTCGTGATGTAAACCAAGTGCCAGAAGGCGTTTCTCGCTTAGAGATTCTACGTAGTGAACCTTATGGG
GAATTTCCAAATCAAGAATACGAGTTCTTTCGTCAATCTTGCCAACATTGTACAAATGCACCTTGTGTAGCAGTTTGTCC
AACTGGTGCATCTTTTATTGATAAAGAGACTGGCATTGTAGATGTACATAAAGATTTATGCATAGGCTGCCAATACTGTA
TAGCAGTTTGCCCATATCGTGTACGTTTTATTCATCCAATACATCGTACCGCAGATAAATGTAACTTCTGCCGCGATACA
AATTTAGCCAATGGTAAACAACCTGCTTGTGTAGAGGCTTGCCCAACCAAAGCATTAACCTTTGGGGATATGAATGATCC
AACAAGTGCAGTTTCACGTAAAGTGAAAGAAAAACCGGTTTATCGCACTAAAGTGGAATTAGGTACACAACCAAACTTAT
ATCACATTCCATTCCAACATGGGGAGCCTAGAAGATGA

Upstream 100 bases:

>100_bases
TTCAACCAAAACAACGCGTTAAACTTTGCGTAGATTGCCATGGTAAACAACAAAAACGTAAAGCTGAACAAGATAAACTA
ACCAAACAAAAGGATAAACA

Downstream 100 bases:

>100_bases
CGTTAGATTATCCAGTTCCATTTCATACGCCTAATCTAGTGTGGGATTATACCATTGCGATCTACTTGTTCTTATTGGGG
ATCTCATCTGGTGCAGTACA

Product: Fe-S-cluster-containing hydrogenase subunit NrfC

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTVCSRRNFVSGMGAVILMTGTSLPSFAKEDKADKPKRYAMIHDENACIGCTACMDACRDVNQVPEGVSRLEILRSEPYG
EFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIVDVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDT
NLANGKQPACVEACPTKALTFGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR

Sequences:

>Translated_225_residues
MTVCSRRNFVSGMGAVILMTGTSLPSFAKEDKADKPKRYAMIHDENACIGCTACMDACRDVNQVPEGVSRLEILRSEPYG
EFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIVDVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDT
NLANGKQPACVEACPTKALTFGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR
>Mature_224_residues
TVCSRRNFVSGMGAVILMTGTSLPSFAKEDKADKPKRYAMIHDENACIGCTACMDACRDVNQVPEGVSRLEILRSEPYGE
FPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIVDVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDTN
LANGKQPACVEACPTKALTFGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=218, Percent_Identity=64.6788990825688, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI226510944, Length=216, Percent_Identity=47.2222222222222, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1789370, Length=223, Percent_Identity=31.8385650224215, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790326, Length=179, Percent_Identity=30.7262569832402, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787749, Length=224, Percent_Identity=28.5714285714286, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787122, Length=193, Percent_Identity=32.1243523316062, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1787872, Length=193, Percent_Identity=32.1243523316062, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787740, Length=145, Percent_Identity=29.6551724137931, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787478, Length=137, Percent_Identity=30.6569343065693, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789079, Length=188, Percent_Identity=28.7234042553192, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789067, Length=160, Percent_Identity=31.875, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082180, Length=166, Percent_Identity=31.3253012048193, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI2367245, Length=173, Percent_Identity=31.2138728323699, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788811, Length=168, Percent_Identity=31.547619047619, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082179, Length=92, Percent_Identity=36.9565217391304, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI87082114, Length=190, Percent_Identity=27.3684210526316, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1787963, Length=197, Percent_Identity=24.8730964467005, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25106; Mature: 24975

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
10.2 %Cys+Met (Translated Protein)
7.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
9.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVCSRRNFVSGMGAVILMTGTSLPSFAKEDKADKPKRYAMIHDENACIGCTACMDACRD
CCCCCCCCHHHCCCEEEEEECCCCCCHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHH
VNQVPEGVSRLEILRSEPYGEFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCEE
DVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDTNLANGKQPACVEACPTKALT
ECHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEE
FGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TVCSRRNFVSGMGAVILMTGTSLPSFAKEDKADKPKRYAMIHDENACIGCTACMDACRD
CCCCCCCHHHCCCEEEEEECCCCCCHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHH
VNQVPEGVSRLEILRSEPYGEFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCEE
DVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDTNLANGKQPACVEACPTKALT
ECHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEE
FGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]