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The map label for this gene is ligA [H]

Identifier: 148826285

GI number: 148826285

Start: 1302987

End: 1304999

Strand: Direct

Name: ligA [H]

Synonym: CGSHiEE_06560

Alternate gene names: 148826285

Gene position: 1302987-1304999 (Clockwise)

Preceding gene: 148826284

Following gene: 148826298

Centisome position: 71.87

GC content: 40.64

Gene sequence:

>2013_bases
ATGACAAATATTCAAACTCAACTAGACAATCTACGCAAAACCTTGCGCCAATATGAATACGAATACCACGTTTTAGATAA
TCCGAGCGTGCCTGATAGCGAATACGATCGTTTATTTCATCAGCTCAAAGCCCTAGAATTAGAGCATCCTGAATTTCTGA
CGTCAGATTCGCCAACTCAACGTGTTGGTGCAAAACCACTTTCTGGGTTTAGCCAAATTCGTCACGAAATTCCTATGCTC
TCTTTGGATAATGCTTTTTCCGATGCAGAATTTAATGCTTTTGTAAAACGCATTGAAGATCGTTTAATCCTATTACCGAA
ACAACTTACTTTCTGTTGCGAACCTAAACTCGATGGCTTGGCTGTGAGTATTTTGTATGTTAATGGTGTACTTACACAAG
CCGCCACTCGTGGCGATGGCACCATAGGCGAAGATATTACGGCAAATATCCGCACGATTCGTAATGTTCCATTACAACTT
CTAACAGATAATCCTCCAGCACGTTTAGAAGTGCGGGGCGAAGTTTTTATGCCGCACGCAGGCTTTGAGCGTTTAAATAA
ATATGCGTTAGAACATAATGAAAAAACCTTTGCTAATCCTCGCAATGCAGCGGCAGGCTCTTTACGCCAGCTTGATCCTA
ATATTACCAGCAAACGTCCGCTGGTATTAAATGCTTATGGTATTGGGATTGCTGAGGGGGTTGATCTGCCGACTACGCAT
TATGCTCGTTTGCAATGGCTAAAATCTATCGGGATTCCAGTAAATCCTGAAATTCGTTTATGCAATGGTGCAGATGAAGT
TTTAGGTTTTTATCAAGATATTCAAAACAAACGTAGCTCGTTAGGTTATGATATTGACGGAACGGTATTAAAAATCAATG
ATATAGCCTTACAAAATGAATTAGGATTTATTTCTAAAGCACCTCGCTGGGCGACTGCTTATAAATTCCCCGCCCAAGAA
GAATTAACCCTGTTGAATGATGTTGAATTCCAAGTGGGTCGAACTGGCGCAATCACACCAGTTGCCAAATTAGAGCCAGT
GTTTGTTGCAGGAGTTACAGTAAGTAATGCCACCTTACATAATGGCGATGAAATTGAACGTTTAAATATCGCTATTGGCG
ATACTGTTGTTATTCGTCGAGCAGGCGATGTAATTCCACAAATTATTGGCGTATTACACGAACGTCGCCCTGATAATGCT
AAACCAATCATTTTCCCAACAAATTGCCCTGTGTGCGATTCTCAAATTATTCGTATTGAAGGCGAAGCAGTAGCACGTTG
CACGGGAGGATTATTCTGTGCGGCACAGCGTAAAGAGGCGTTAAAACATTTCGTCTCTCGTAAAGCAATGGATATTGATG
GTGTAGGCGGAAAATTAATAGAGCAATTAGTCGATCGAGAACTGATTCATACACCTGCGGACTTATTTAAACTTGATTTA
ACCACGCTCACACGCTTAGAAAGAATGGGCACAAAATCTGCAGAAAACGCACTCAATAGTCTTGAAAATGCAAAAAGCAC
GACGCTTGCTCGCTTTATTTTTGCTTTAGGCATTCGTGAAGTGGGCGAAGCCACTGCATTAAATCTTGCTAATCATTTCA
AAACTTTAGATGCGCTTAAAGACGCAAATCTTGAAGAACTTCAACAAGTGCCTGATGTAGGGGAAGTTGTAGCGAACCGA
ATTTTTATATTTTGGCGTGAAGCGCATAATGTTGCAGTTGTAGAGGATTTAATCGCACAAGGTGTGCATTGGGAAACTGT
AGAAGTGAAAGAAGCAAGTGAAAACTTGTTCAAAGATAAAACTGTAGTACTGACAGGCACTCTTACACAAATGGGGCGTA
ATGAAGCAAAAGCCTTATTACAACAGCTTGGTGCAAAAGTAAGTGGTTCGGTATCCAGTAAAACAGATTTCGTTATCGCA
GGCGATGCGGCAGGCTCTAAACTTGCAAAAGCTCAAGAACTAAATATTACAGTATTAACAGAAGAAGAATTTTTAGCACA
GATAACAAGATAA

Upstream 100 bases:

>100_bases
AAATTTACCGCACTTTTATATTCATTTAGACAGGCTTTGTACCTGTCTATTTTCACACTAAATACATAGAAAAGTGCGGT
CATTTTTCTCCGAGAATTTT

Downstream 100 bases:

>100_bases
TTTTTTATCTAAAATAGTGATAATTATAATGATACCCGACGCTTGGTGTAACTGGAAGAACAGGCAATACACGTCTTGTT
CTCGTTTTAACTTTATTGTC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MTNIQTQLDNLRKTLRQYEYEYHVLDNPSVPDSEYDRLFHQLKALELEHPEFLTSDSPTQRVGAKPLSGFSQIRHEIPML
SLDNAFSDAEFNAFVKRIEDRLILLPKQLTFCCEPKLDGLAVSILYVNGVLTQAATRGDGTIGEDITANIRTIRNVPLQL
LTDNPPARLEVRGEVFMPHAGFERLNKYALEHNEKTFANPRNAAAGSLRQLDPNITSKRPLVLNAYGIGIAEGVDLPTTH
YARLQWLKSIGIPVNPEIRLCNGADEVLGFYQDIQNKRSSLGYDIDGTVLKINDIALQNELGFISKAPRWATAYKFPAQE
ELTLLNDVEFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLNIAIGDTVVIRRAGDVIPQIIGVLHERRPDNA
KPIIFPTNCPVCDSQIIRIEGEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGGKLIEQLVDRELIHTPADLFKLDL
TTLTRLERMGTKSAENALNSLENAKSTTLARFIFALGIREVGEATALNLANHFKTLDALKDANLEELQQVPDVGEVVANR
IFIFWREAHNVAVVEDLIAQGVHWETVEVKEASENLFKDKTVVLTGTLTQMGRNEAKALLQQLGAKVSGSVSSKTDFVIA
GDAAGSKLAKAQELNITVLTEEEFLAQITR

Sequences:

>Translated_670_residues
MTNIQTQLDNLRKTLRQYEYEYHVLDNPSVPDSEYDRLFHQLKALELEHPEFLTSDSPTQRVGAKPLSGFSQIRHEIPML
SLDNAFSDAEFNAFVKRIEDRLILLPKQLTFCCEPKLDGLAVSILYVNGVLTQAATRGDGTIGEDITANIRTIRNVPLQL
LTDNPPARLEVRGEVFMPHAGFERLNKYALEHNEKTFANPRNAAAGSLRQLDPNITSKRPLVLNAYGIGIAEGVDLPTTH
YARLQWLKSIGIPVNPEIRLCNGADEVLGFYQDIQNKRSSLGYDIDGTVLKINDIALQNELGFISKAPRWATAYKFPAQE
ELTLLNDVEFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLNIAIGDTVVIRRAGDVIPQIIGVLHERRPDNA
KPIIFPTNCPVCDSQIIRIEGEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGGKLIEQLVDRELIHTPADLFKLDL
TTLTRLERMGTKSAENALNSLENAKSTTLARFIFALGIREVGEATALNLANHFKTLDALKDANLEELQQVPDVGEVVANR
IFIFWREAHNVAVVEDLIAQGVHWETVEVKEASENLFKDKTVVLTGTLTQMGRNEAKALLQQLGAKVSGSVSSKTDFVIA
GDAAGSKLAKAQELNITVLTEEEFLAQITR
>Mature_669_residues
TNIQTQLDNLRKTLRQYEYEYHVLDNPSVPDSEYDRLFHQLKALELEHPEFLTSDSPTQRVGAKPLSGFSQIRHEIPMLS
LDNAFSDAEFNAFVKRIEDRLILLPKQLTFCCEPKLDGLAVSILYVNGVLTQAATRGDGTIGEDITANIRTIRNVPLQLL
TDNPPARLEVRGEVFMPHAGFERLNKYALEHNEKTFANPRNAAAGSLRQLDPNITSKRPLVLNAYGIGIAEGVDLPTTHY
ARLQWLKSIGIPVNPEIRLCNGADEVLGFYQDIQNKRSSLGYDIDGTVLKINDIALQNELGFISKAPRWATAYKFPAQEE
LTLLNDVEFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLNIAIGDTVVIRRAGDVIPQIIGVLHERRPDNAK
PIIFPTNCPVCDSQIIRIEGEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGGKLIEQLVDRELIHTPADLFKLDLT
TLTRLERMGTKSAENALNSLENAKSTTLARFIFALGIREVGEATALNLANHFKTLDALKDANLEELQQVPDVGEVVANRI
FIFWREAHNVAVVEDLIAQGVHWETVEVKEASENLFKDKTVVLTGTLTQMGRNEAKALLQQLGAKVSGSVSSKTDFVIAG
DAAGSKLAKAQELNITVLTEEEFLAQITR

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=667, Percent_Identity=63.568215892054, Blast_Score=880, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=585, Percent_Identity=22.3931623931624, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74046; Mature: 73915

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIQTQLDNLRKTLRQYEYEYHVLDNPSVPDSEYDRLFHQLKALELEHPEFLTSDSPTQ
CCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RVGAKPLSGFSQIRHEIPMLSLDNAFSDAEFNAFVKRIEDRLILLPKQLTFCCEPKLDGL
HCCCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHEEEECCHHCEEECCCCCCE
AVSILYVNGVLTQAATRGDGTIGEDITANIRTIRNVPLQLLTDNPPARLEVRGEVFMPHA
EEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEEEECCEECCCC
GFERLNKYALEHNEKTFANPRNAAAGSLRQLDPNITSKRPLVLNAYGIGIAEGVDLPTTH
CHHHHHHHHHHCCCCCCCCCCCHHCCCHHHCCCCCCCCCCEEEEEECCCEECCCCCCCHH
YARLQWLKSIGIPVNPEIRLCNGADEVLGFYQDIQNKRSSLGYDIDGTVLKINDIALQNE
HHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCC
LGFISKAPRWATAYKFPAQEELTLLNDVEFQVGRTGAITPVAKLEPVFVAGVTVSNATLH
CCCCCCCCCCCEEECCCCCHHHHHHHCCEEEECCCCCCCCHHHCCCEEEEEEEEECCEEC
NGDEIERLNIAIGDTVVIRRAGDVIPQIIGVLHERRPDNAKPIIFPTNCPVCDSQIIRIE
CCCCEEEEEEEECCEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEC
GEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGGKLIEQLVDRELIHTPADLFKLDL
CCHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
TTLTRLERMGTKSAENALNSLENAKSTTLARFIFALGIREVGEATALNLANHFKTLDALK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DANLEELQQVPDVGEVVANRIFIFWREAHNVAVVEDLIAQGVHWETVEVKEASENLFKDK
CCCHHHHHHCCCHHHHHHCEEEEEEECCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCC
TVVLTGTLTQMGRNEAKALLQQLGAKVSGSVSSKTDFVIAGDAAGSKLAKAQELNITVLT
EEEEEECHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCEEEEEE
EEEFLAQITR
CHHHHHHHCC
>Mature Secondary Structure 
TNIQTQLDNLRKTLRQYEYEYHVLDNPSVPDSEYDRLFHQLKALELEHPEFLTSDSPTQ
CCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RVGAKPLSGFSQIRHEIPMLSLDNAFSDAEFNAFVKRIEDRLILLPKQLTFCCEPKLDGL
HCCCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHEEEECCHHCEEECCCCCCE
AVSILYVNGVLTQAATRGDGTIGEDITANIRTIRNVPLQLLTDNPPARLEVRGEVFMPHA
EEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEEEECCEECCCC
GFERLNKYALEHNEKTFANPRNAAAGSLRQLDPNITSKRPLVLNAYGIGIAEGVDLPTTH
CHHHHHHHHHHCCCCCCCCCCCHHCCCHHHCCCCCCCCCCEEEEEECCCEECCCCCCCHH
YARLQWLKSIGIPVNPEIRLCNGADEVLGFYQDIQNKRSSLGYDIDGTVLKINDIALQNE
HHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCC
LGFISKAPRWATAYKFPAQEELTLLNDVEFQVGRTGAITPVAKLEPVFVAGVTVSNATLH
CCCCCCCCCCCEEECCCCCHHHHHHHCCEEEECCCCCCCCHHHCCCEEEEEEEEECCEEC
NGDEIERLNIAIGDTVVIRRAGDVIPQIIGVLHERRPDNAKPIIFPTNCPVCDSQIIRIE
CCCCEEEEEEEECCEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEC
GEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGGKLIEQLVDRELIHTPADLFKLDL
CCHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
TTLTRLERMGTKSAENALNSLENAKSTTLARFIFALGIREVGEATALNLANHFKTLDALK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DANLEELQQVPDVGEVVANRIFIFWREAHNVAVVEDLIAQGVHWETVEVKEASENLFKDK
CCCHHHHHHCCCHHHHHHCEEEEEEECCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCC
TVVLTGTLTQMGRNEAKALLQQLGAKVSGSVSSKTDFVIAGDAAGSKLAKAQELNITVLT
EEEEEECHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCEEEEEE
EEEFLAQITR
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA