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The map label for this gene is rfaF [H]

Identifier: 148826280

GI number: 148826280

Start: 1297020

End: 1298060

Strand: Direct

Name: rfaF [H]

Synonym: CGSHiEE_06535

Alternate gene names: 148826280

Gene position: 1297020-1298060 (Clockwise)

Preceding gene: 148826279

Following gene: 148826284

Centisome position: 71.54

GC content: 41.69

Gene sequence:

>1041_bases
ATGAATATTCTAATTATTGGCCCATCTTGGGTTGGCGATATGATGATGTCGCACAGTTTGTACCAACAACTGAAAATTCA
ATATCCAAACTGCAATATTGATGTGATGGCACCGAATTGGTGTAAGCCTTTGCTTGCGCGTATGCCAGAAGTGCGTAAGG
CGATTGAAATGCCATTAGGGCACGGAGCCTTTGAATTGGGAACGCGTTATCGTTTAGGTAAGGCATTGCGCAATCAATAT
GATATGGCGATCGTATTACCAAACTCCTTGAAGTCAGCTTTTATTCCCGCTTTTGCAAAAATTGCGGTGCGTCGTGGCTG
GAAAGGGGAAAGCCGTTATTTCTTGCTTAACGATTTACGCAATAATAAACGTGATTATCCTATGATGGTGCAACGTTATG
TCGCGTTAGCTTTTGAGCAAAATGCGGTACCGAAAGCGGCTGATATTCCTGTTCTAAAACCTTATTTAACCGTTGATCCC
ACTCAACAAGCTGAAACCTTAAAAAACTTTAAAAAACAAACCGCACTTTTAGGCGAGCGTCCAATTATTGGTTTTTGTCC
TGGTGCGGAATTTGGCCCGGCAAAACGTTGGCCACATTATCATTATGCAAAATTAGCAGAAATGTTAATTACCCAAGGCT
ATGCTGTGGCGCTATTTGGTTCAGCTAAAGATGAACCAGTGGGAGAAGAAATTCGTCAAGCACTGCCAGAAGCGTTGCGT
GAGTTTTGTGTCAATTTAGCAGGAAAAACCAACTTAAATGAAGCAGTGGATTTAATTGCAAATTGTACGGCGGTCGTGAC
CAATGACAGTGGTTTAATGCATATTGCGGCAGCAGTAAATCGTCCATTGATTGCACTTTATGGACCAACTAGCCCACAAT
ATACGCCGCCGCTTTCAGATAAAGCAACGATTATTCGTTTAATTGAAGGTGGATTGATTAAAGTCCGTAAAGGCGACAAA
GAGGGGGGGTATCATCAAAGTTTGATTGATATTACGCCAGAAATGGCATTAGAAAAATTGAACGAACTGTTGGCAAAATA
A

Upstream 100 bases:

>100_bases
TAAAGAGTTTCGACTAAATTGTTAAAGGAGCATATTTTTATTCAACTCTGTGCCATAATAACAAGTATTTTTGACCGCAC
TTTTATCTGAAAGACAGAAA

Downstream 100 bases:

>100_bases
TGAAAGTTTGCTTAGTGAAAAGAAAACTTATAAAGATATTACTTATAAGATGACTTGTTTCATAAAAAATGCTCGCACTT
GGCGAGCATTTTTATCTTTT

Product: ADP-heptose--lipooligosaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLGHGAFELGTRYRLGKALRNQY
DMAIVLPNSLKSAFIPAFAKIAVRRGWKGESRYFLLNDLRNNKRDYPMMVQRYVALAFEQNAVPKAADIPVLKPYLTVDP
TQQAETLKNFKKQTALLGERPIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEALR
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSDKATIIRLIEGGLIKVRKGDK
EGGYHQSLIDITPEMALEKLNELLAK

Sequences:

>Translated_346_residues
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLGHGAFELGTRYRLGKALRNQY
DMAIVLPNSLKSAFIPAFAKIAVRRGWKGESRYFLLNDLRNNKRDYPMMVQRYVALAFEQNAVPKAADIPVLKPYLTVDP
TQQAETLKNFKKQTALLGERPIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEALR
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSDKATIIRLIEGGLIKVRKGDK
EGGYHQSLIDITPEMALEKLNELLAK
>Mature_346_residues
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLGHGAFELGTRYRLGKALRNQY
DMAIVLPNSLKSAFIPAFAKIAVRRGWKGESRYFLLNDLRNNKRDYPMMVQRYVALAFEQNAVPKAADIPVLKPYLTVDP
TQQAETLKNFKKQTALLGERPIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEALR
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSDKATIIRLIEGGLIKVRKGDK
EGGYHQSLIDITPEMALEKLNELLAK

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=348, Percent_Identity=61.4942528735632, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1790062, Length=319, Percent_Identity=25.0783699059561, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38564; Mature: 38564

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLG
CEEEEECCHHHHHHHHHHHHHHHHEEECCCCCEEEECCHHHHHHHHHCHHHHHHHHCCCC
HGAFELGTRYRLGKALRNQYDMAIVLPNSLKSAFIPAFAKIAVRRGWKGESRYFLLNDLR
CCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHC
NNKRDYPMMVQRYVALAFEQNAVPKAADIPVLKPYLTVDPTQQAETLKNFKKQTALLGER
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCC
PIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEALR
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSD
HHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCEEEEHHHCCCEEEEECCCCCCCCCCCCC
KATIIRLIEGGLIKVRKGDKEGGYHQSLIDITPEMALEKLNELLAK
HHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLG
CEEEEECCHHHHHHHHHHHHHHHHEEECCCCCEEEECCHHHHHHHHHCHHHHHHHHCCCC
HGAFELGTRYRLGKALRNQYDMAIVLPNSLKSAFIPAFAKIAVRRGWKGESRYFLLNDLR
CCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHC
NNKRDYPMMVQRYVALAFEQNAVPKAADIPVLKPYLTVDPTQQAETLKNFKKQTALLGER
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCC
PIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEALR
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSD
HHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCEEEEHHHCCCEEEEECCCCCCCCCCCCC
KATIIRLIEGGLIKVRKGDKEGGYHQSLIDITPEMALEKLNELLAK
HHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]