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The map label for this gene is ptsN [H]

Identifier: 148826241

GI number: 148826241

Start: 1249856

End: 1250350

Strand: Direct

Name: ptsN [H]

Synonym: CGSHiEE_06310

Alternate gene names: 148826241

Gene position: 1249856-1250350 (Clockwise)

Preceding gene: 148826240

Following gene: 148826242

Centisome position: 68.94

GC content: 36.16

Gene sequence:

>495_bases
ATGAAAATAACTGAACTATTAAGCCCTGAGAATATTCGTCAGGGCGTTAGTTTTTCTAGCAAAAAACGATTATTTGAGTC
TATTGCGCATTTTGTAGAAGAACAAATCCTTGCAGAACAAGGCGAACAAGCCTGTTTTGAATGCCTTTTTGAACGCGAAA
AACTGGGGAATTCTGGTTTAGGAAATGGGATTGCAATGCCAAAAGCGAAAATCCCCGTAACAGTATCAGATAAAGCAATC
GCAGTGTTTATGCAACTCGATAATCCGATTGATTACGATGCTTTTGATGGTAAACCAGTGGATTTAATCTTTGCCTTGCT
GATTCCAGAAAATCAATGTGAAACCTACATTCCAGTTTTAGCATCATTAATAGAAAAACTGACTGATAAAAATGTGTTGA
AACAATTACGATCAGCCAAAAGTGCGGATGAAATTTGGCAAGTTTTTGAAATTACCGATCAATCAGAAACGACATTTGAA
GAAGTGAAAGAATAA

Upstream 100 bases:

>100_bases
TTGGCGCAGGTAAAATTATCGCAACAGGCACACCAGAACAAGTGATGAACGATGAACAGGTTAAACGCGTTTATCTTGGC
GAACAATTCAAATTATAAAA

Downstream 100 bases:

>100_bases
TTACAGTGGGGAAACTTATGGAAATTATCATTATTAGTGGACGTTCTGGCGCAGGAAAATCCGTAGCCTTACGAGCATTG
GAAGATACAGGATATTATTG

Product: nitrogen regulatory IIA protein

Products: protein histidine; sugar phosphate

Alternate protein names: Putative phosphotransferase enzyme IIA component; Putative PTS system EIIA component [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEQGEQACFECLFEREKLGNSGLGNGIAMPKAKIPVTVSDKAI
AVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVLASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFE
EVKE

Sequences:

>Translated_164_residues
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEQGEQACFECLFEREKLGNSGLGNGIAMPKAKIPVTVSDKAI
AVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVLASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFE
EVKE
>Mature_164_residues
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEQGEQACFECLFEREKLGNSGLGNGIAMPKAKIPVTVSDKAI
AVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVLASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFE
EVKE

Specific function: Not known; lacks the phosphorylation site found in other ptsN proteins [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=157, Percent_Identity=38.8535031847134, Blast_Score=120, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 18444; Mature: 18444

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEQGEQACFECLFEREKLGNSGL
CCCHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
GNGIAMPKAKIPVTVSDKAIAVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVL
CCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
ASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFEEVKE
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEQGEQACFECLFEREKLGNSGL
CCCHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
GNGIAMPKAKIPVTVSDKAIAVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVL
CCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
ASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFEEVKE
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]