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The map label for this gene is ackA [H]

Identifier: 148826187

GI number: 148826187

Start: 1195529

End: 1196734

Strand: Direct

Name: ackA [H]

Synonym: CGSHiEE_06020

Alternate gene names: 148826187

Gene position: 1195529-1196734 (Clockwise)

Preceding gene: 148826184

Following gene: 148826188

Centisome position: 65.94

GC content: 40.22

Gene sequence:

>1206_bases
ATGTCAAAACTTGTTCTTATCCTTAACTGCGGTAGTTCTTCACTAAAATTTGCAATTCTTGATCCAGCAACAGGGGAAGA
AAAATTATCTGGCTTAGCCGAAGCATTTTTCTTACCAGAAGCACGTATCAAATGGAAACTCAATGGAGAGAAAGGTAACG
CAGATTTAGGCGCAGGTGCAGCGCATACTGAAGCGCTTAATTTTATTGCATCTAACATTCTGACTGATGAACTAAAAAAT
TCTATCGCAGCAATTGGTCACCGTATCGTTCACGGTGGCGAAAAATACACCCAATCTGTTATCGTAACTGATGAAGTTGT
TAAAGGTATTGAAGATGCTGCACAATTTGCGCCACTTCACAACCCTGCTCACTTAATTGGTATTCGCGAAGCATTTAACG
CATTCCCACACTTAAAAGATAAAAACGTGGTAGTTTTTGATACAGCATTCCACCAAACTATGTCTGAAGAAGCATTCCTT
TATGCCCTTCCATACTCTTTATATAAAGAACACGGCGTACGTCGCTATGGTGCACACGGCACAAGTCACTACTTCATTTC
TCGTGAAGTCGCTGAATATGTAGGCAAACCCGCAGACCAAGTCAATGCTATTATTTGTCACTTAGGTAACGGTGGTTCTG
TTTCTGTTGTGCGTAACGGTCAATGTATCGATACGTCAATGGGTTTAACTCCGCTAGAAGGTTTGGTAATGGGTACACGT
TGTGGCGATATTGACCCTGCAATCATATTCTATCTATACAAAACTTTAGGTATGTCTATGGAACAAATCGAAGAGACTTT
AGTGAAAAAATCTGGTCTTTTAGGCTTAACTGAAGTAACCAGCGACTGCCGTTATGCTGAAGATAACTACGATAATGCAT
CTAAACCAGAAGCAAAACGCGCTTTAAATGTTTATAGCTATCGTTTAGCGAAATACATTGGTGCTTATATGGCTGTGTTG
GGCGATGATCACTTAGATGCGATTGCATTCACTGGTGGTATTGGCGAAAACTCAGCGCACGTTCGTGAATTAGCATTGAA
TCACTTAAAATTATTTGGCATTCAAATCGATAATGAACGAAATCTTGCCGCACGTTTTGGTAAAGATGGCGTAATCACGA
CTGATGATTCCGCATTTAAAGCGATTGTTCTTCCAACTAACGAAGAATTGGTTATCGCACAAGATACCGCAAGACTTTGT
TTCTAA

Upstream 100 bases:

>100_bases
TGAATTTATTTTATTCATCAAAAGGTGTATCCTATACCTAATTTTTCAAACCATCTTAAATGATGGATTTCTATTAACCT
CAATCAAATATAGGGTTCCT

Downstream 100 bases:

>100_bases
TTCAACAACAAACTGCCGAATTTTTCGGCAGTTTTTATTTATAACATATACATATTAAGGTTTAACTATGTCTCGCACGA
TTATTCTTATCCCTGTAAGT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGAAHTEALNFIASNILTDELKN
SIAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLHNPAHLIGIREAFNAFPHLKDKNVVVFDTAFHQTMSEEAFL
YALPYSLYKEHGVRRYGAHGTSHYFISREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTR
CGDIDPAIIFYLYKTLGMSMEQIEETLVKKSGLLGLTEVTSDCRYAEDNYDNASKPEAKRALNVYSYRLAKYIGAYMAVL
GDDHLDAIAFTGGIGENSAHVRELALNHLKLFGIQIDNERNLAARFGKDGVITTDDSAFKAIVLPTNEELVIAQDTARLC
F

Sequences:

>Translated_401_residues
MSKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGAAHTEALNFIASNILTDELKN
SIAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLHNPAHLIGIREAFNAFPHLKDKNVVVFDTAFHQTMSEEAFL
YALPYSLYKEHGVRRYGAHGTSHYFISREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTR
CGDIDPAIIFYLYKTLGMSMEQIEETLVKKSGLLGLTEVTSDCRYAEDNYDNASKPEAKRALNVYSYRLAKYIGAYMAVL
GDDHLDAIAFTGGIGENSAHVRELALNHLKLFGIQIDNERNLAARFGKDGVITTDDSAFKAIVLPTNEELVIAQDTARLC
F
>Mature_400_residues
SKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGAAHTEALNFIASNILTDELKNS
IAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLHNPAHLIGIREAFNAFPHLKDKNVVVFDTAFHQTMSEEAFLY
ALPYSLYKEHGVRRYGAHGTSHYFISREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTRC
GDIDPAIIFYLYKTLGMSMEQIEETLVKKSGLLGLTEVTSDCRYAEDNYDNASKPEAKRALNVYSYRLAKYIGAYMAVLG
DDHLDAIAFTGGIGENSAHVRELALNHLKLFGIQIDNERNLAARFGKDGVITTDDSAFKAIVLPTNEELVIAQDTARLCF

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=69, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=41.9191919191919, Blast_Score=308, Evalue=3e-85,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43645; Mature: 43514

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGA
CCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
AHTEALNFIASNILTDELKNSIAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEHHHHHHHHHHHHHHCCCC
NPAHLIGIREAFNAFPHLKDKNVVVFDTAFHQTMSEEAFLYALPYSLYKEHGVRRYGAHG
CCHHEEEHHHHHHHCCCCCCCCEEEEECHHHHHHCCCCEEEECCHHHHHHCCCEECCCCC
TSHYFISREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTR
CCEEEEHHHHHHHHCCCHHHCCEEEEECCCCCEEEEEECCEEEECCCCCCCHHHEEEECC
CGDIDPAIIFYLYKTLGMSMEQIEETLVKKSGLLGLTEVTSDCRYAEDNYDNASKPEAKR
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCHHHH
ALNVYSYRLAKYIGAYMAVLGDDHLDAIAFTGGIGENSAHVRELALNHLKLFGIQIDNER
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCC
NLAARFGKDGVITTDDSAFKAIVLPTNEELVIAQDTARLCF
CHHHHCCCCCCEECCCCCEEEEEECCCCCEEEEECCHHHCC
>Mature Secondary Structure 
SKLVLILNCGSSSLKFAILDPATGEEKLSGLAEAFFLPEARIKWKLNGEKGNADLGAGA
CCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
AHTEALNFIASNILTDELKNSIAAIGHRIVHGGEKYTQSVIVTDEVVKGIEDAAQFAPLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEHHHHHHHHHHHHHHCCCC
NPAHLIGIREAFNAFPHLKDKNVVVFDTAFHQTMSEEAFLYALPYSLYKEHGVRRYGAHG
CCHHEEEHHHHHHHCCCCCCCCEEEEECHHHHHHCCCCEEEECCHHHHHHCCCEECCCCC
TSHYFISREVAEYVGKPADQVNAIICHLGNGGSVSVVRNGQCIDTSMGLTPLEGLVMGTR
CCEEEEHHHHHHHHCCCHHHCCEEEEECCCCCEEEEEECCEEEECCCCCCCHHHEEEECC
CGDIDPAIIFYLYKTLGMSMEQIEETLVKKSGLLGLTEVTSDCRYAEDNYDNASKPEAKR
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCHHHH
ALNVYSYRLAKYIGAYMAVLGDDHLDAIAFTGGIGENSAHVRELALNHLKLFGIQIDNER
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCC
NLAARFGKDGVITTDDSAFKAIVLPTNEELVIAQDTARLCF
CHHHHCCCCCCEECCCCCEEEEEECCCCCEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA