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The map label for this gene is pykA [H]

Identifier: 148826095

GI number: 148826095

Start: 1089578

End: 1091014

Strand: Direct

Name: pykA [H]

Synonym: CGSHiEE_05445

Alternate gene names: 148826095

Gene position: 1089578-1091014 (Clockwise)

Preceding gene: 148826093

Following gene: 148826096

Centisome position: 60.1

GC content: 40.01

Gene sequence:

>1437_bases
ATGTCTAGAAGACTAAGAAGAACGAAGATTGTATGTACTATGGGCCCATCAACTGACCGTGATAACAATCTTGAAAAAAT
TATCGCAGCGGGCGCAAACGTAGTTCGTATGAACTTCTCTCACGGTACACCTGATGACCATATCGGACGTGCTGAACGTG
TACGTTCTATTGCGAAAAAATTAGGTAAAACCGTGGCAATCTTAGGTGATTTACAAGGTCCTAAAATTCGTGTTTCTACT
TTTAAAGACGGTAAAATTTTCTTAAACGTTGGCGATAAATTCATTCTTGATGCAGAGTTACCAAAAGGCGAAGGCACTCA
AGAATCCGTTGGTTTAGACTATAAAACGCTTCCTCAAGATGTTGTGCCTGGCGATATTCTTTTATTAGATGATGGCCGTG
TTCAATTAAAAGTATTATCAACTGATGGTGCAAAAGTTTTCACTGAAGTTACTGTTGGTGGTCCATTATCAAATAATAAA
GGTATCAATAAATTAGGTGGCGGTTTATCTGCGGATGCCCTAACAGAAAAAGATAAAGCCGACATTATTACCGCTGCACG
CATTGGTGTTGATTTCTTAGCCGTTTCTTTCCCTCGTTCAAGTGCAGATTTAAATTATGCACGTGAACTTGCTCAACAAG
CAGGTTTAAATGCAAAAATCGTTGCTAAAGTTGAACGTGCAGAAACCGTTGCTAATGATGAAGCCATGGACGATATTATT
TTAGCATCTGATGTAATTATGGTTGCTCGTGGTGACTTAGGCGTAGAAATCGGCGATCCTGAATTAGTCGGTGTACAGAA
AAAATTAATTCGTCGTTCACGTCAATTAAATCGTGCTGTAATTACAGCGACTCAAATGATGGAATCAATGATTAGTAACC
CAATGCCAACGCGTGCTGAAGTAATGGACGTTGCAAACGCAGTATTAGATGGAACTGATGCAGTTATGCTTTCTGCAGAA
ACAGCAGCAGGTCAATATCCTTCTGAAACAGTGGCAGCAATGGCTAGCGTATGTTTAGGTGCAGAAAAAATGCCAAGCAT
TAACGTTTCTCGTCACCGTATGGATAAAGAATTTGAAACCATTGAAGAATCTGTTGCGATGTCTGCAATGTATGCAGCAA
ACCACATGAAAGGTGTAGCGGCAATCGTCACTTTAACTAGCACAGGCCGTACTCCATTATTAATGTCACGCATTAGCTCT
GGCTTACCAATCTTTGCTTTATCTCGTAATCAAGAAACCCTAAACCTTTGTGCACTATACCGCGGTGTAACACCAATTTA
TCACGGTGAAGAAAGTCGTACAGAAGCAGGTGCAAAAGCAGCACTTCAATCATTAAAAGAAAAAGGTTATTTATCTACTG
GCGATTTAGTACTGGTAACCCAAGGTGGTCAAGGTGCGACACAAACTAACGTATGTCGTACATTAATTGTTGAATAA

Upstream 100 bases:

>100_bases
AAGTTTTAAGCGTATAATGTGCACCAATTTTTTATCTGGCTAATATGTAGCGAAGTAAAATAACACCTTTAAAATTTTGT
AATTATTGACGGAGTAAATA

Downstream 100 bases:

>100_bases
TCAACAATCTAAATATGTTAAAATAAAAGAGCGGTGGATTTTTCCACCGTTTTTTATTTCCTTTTTTCACCGCACTTTTC
ACTTAAATCCAATAGATATT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKKLGKTVAILGDLQGPKIRVST
FKDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNK
GINKLGGGLSADALTEKDKADIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDII
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFETIEESVAMSAMYAANHMKGVAAIVTLTSTGRTPLLMSRISS
GLPIFALSRNQETLNLCALYRGVTPIYHGEESRTEAGAKAALQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE

Sequences:

>Translated_478_residues
MSRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKKLGKTVAILGDLQGPKIRVST
FKDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNK
GINKLGGGLSADALTEKDKADIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDII
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFETIEESVAMSAMYAANHMKGVAAIVTLTSTGRTPLLMSRISS
GLPIFALSRNQETLNLCALYRGVTPIYHGEESRTEAGAKAALQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE
>Mature_477_residues
SRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKKLGKTVAILGDLQGPKIRVSTF
KDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNKG
INKLGGGLSADALTEKDKADIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDIIL
ASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFETIEESVAMSAMYAANHMKGVAAIVTLTSTGRTPLLMSRISSG
LPIFALSRNQETLNLCALYRGVTPIYHGEESRTEAGAKAALQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=498, Percent_Identity=35.5421686746988, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI33286420, Length=498, Percent_Identity=35.5421686746988, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI33286418, Length=497, Percent_Identity=35.010060362173, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI10835121, Length=497, Percent_Identity=34.0040241448692, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI32967597, Length=497, Percent_Identity=34.0040241448692, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=34.6020761245675, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=34.6020761245675, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=32.3404255319149, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=32.3404255319149, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=31.7757009345794, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=77.2916666666667, Blast_Score=755, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=39.0295358649789, Blast_Score=292, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=499, Percent_Identity=35.6713426853707, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI71984413, Length=492, Percent_Identity=35.5691056910569, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17506831, Length=492, Percent_Identity=35.5691056910569, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=492, Percent_Identity=35.5691056910569, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI71984406, Length=492, Percent_Identity=35.5691056910569, Blast_Score=238, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=482, Percent_Identity=36.7219917012448, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=33.4710743801653, Blast_Score=242, Evalue=8e-65,
Organism=Drosophila melanogaster, GI28571814, Length=502, Percent_Identity=37.0517928286853, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24648964, Length=502, Percent_Identity=37.0517928286853, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=36.8550368550369, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=31.6326530612245, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24646914, Length=281, Percent_Identity=33.8078291814947, Blast_Score=138, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51085; Mature: 50954

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKK
CCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHH
LGKTVAILGDLQGPKIRVSTFKDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCHHHCCCCHHHCCCC
VVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNKGINKLGGGLSADALTEKDKA
CCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCCCCHHHCCCCHH
DIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDII
HEEHHHHHCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCHHHHHHH
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAE
HHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFET
HHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IEESVAMSAMYAANHMKGVAAIVTLTSTGRTPLLMSRISSGLPIFALSRNQETLNLCALY
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHH
RGVTPIYHGEESRTEAGAKAALQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE
HCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKK
CCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHH
LGKTVAILGDLQGPKIRVSTFKDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCHHHCCCCHHHCCCC
VVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNKGINKLGGGLSADALTEKDKA
CCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCCCCHHHCCCCHH
DIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDII
HEEHHHHHCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCHHHHHHH
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAE
HHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFET
HHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IEESVAMSAMYAANHMKGVAAIVTLTSTGRTPLLMSRISSGLPIFALSRNQETLNLCALY
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHH
RGVTPIYHGEESRTEAGAKAALQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE
HCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]