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The map label for this gene is mnmC [H]

Identifier: 148826060

GI number: 148826060

Start: 1055527

End: 1057539

Strand: Direct

Name: mnmC [H]

Synonym: CGSHiEE_05250

Alternate gene names: 148826060

Gene position: 1055527-1057539 (Clockwise)

Preceding gene: 148826058

Following gene: 148826061

Centisome position: 58.22

GC content: 40.59

Gene sequence:

>2013_bases
ATGACCTTTAGCGTGCAACATGCTGAGATTCACTTCAACCAAAACCATATTCCCGTCTCAGATCAATTCGATGATGTCTA
TTTTTCTAACGAAAATGGATTGGCAGAAACCGATTATGTTTTTCTACAAGGTAATCAACTTTGGGAACGTTGGATAACAC
ACAAAGAAGCTAATTTTGTCATCGCTGAAACAGGATTTGGTACAGGATTAAATTTCTTTGCGGTAACCAAATTGTTCCGT
GAATTTCGCCAGCAACACGAGAATCATCCTTTAAAACGCCTAAATTTTATTTCCTTTGAAAAATATCCGCTAAAAATCAC
CGCACTTTTGCAAGCGCATCTTGCCTATCCGCAGTTTGAAGATTTAAGCGCTCATTTGCAACGTTATTGGCCTTCATTGA
TTCTAGGTTGTCATCGTATTCATTTTGGAGAGACAACCTTAGATTTATGGCTTGGTGATGTATCAGAAAACTTGCCACAA
CTTGGCGATTATATGAATGAACGTATTGATGCTTGGTTTTTAGATGGCTTTGCGCCGAGTAAAAATCCTGAAATGTGGAA
TGATGATCTCTATAACTTGATGTTTCGTTTCACGAAACCCAATGGTTCTTTTGCTACCTTCACCGCAGCAAGTGCGGTCA
GAAAAGGACTTGAATCCGCAGGCTTTAACGTGACAAAACGTAAAGGCTTCGGCAAAAAGCGAGAATGTTTAAGTGGGTTA
AAAATACAATCAAAATCTACCGCACTTTCAACACCTTGGTATCTTGCGCAGCCTGCGAAAATGGAACAACAAGATGTCGC
TATTATCGGCGGTGGTATTGCCTCTCTTTGTGCTGCTATTTCCTTAGTAAAACGTGGGGCAAAAGTCACCATTTATTGTG
AAGATGATTCGCTCGCACTCAATGCTTCGGGCAATAAACAAGGCGCATTTTATCCGCAACTTAGCGATGATAATGCGCTT
ACCGTTGATTTTTACCTGCACGCTTTCAGTTACGGTCGCCAATTACTTGATTGGGCAATCGCACAAAACATAGCGTTTGA
ACATGAATTTTGTGGCGTGGCACTATGTGCTTACAACAAAAAAAGTGCGGTCAAATTAGCAAAGATTTCTCAGCTTGGTT
TGCCGAACGAAATCTTCCAAATGCTGAATGCTGAACAACTCAGTGAAAAAGTGGGATTACCGCTTAATTGCAAAGGCGGT
TGGATTGAACAAGGGGCTTGGCTTGCACCAAGACAATTTGTTCAAAATGCCTTTTCATTTCTTGAAAAGCAGGGTGTCAT
CATTAAAACCTCACAAAAAATTACCGCACTTTCTCAGCTAGAAAAAGGTTGGGAACTAAAAAATATGCAAGGTCAAAAAT
ACTGCCATGAAGTCGTCATTCTTGCGAACGGATATAAAATTACTGATTTTGTTCAAACAGAAAAGCTACCGCTCTACCCT
ATCCGCGGGCAAGTCAGCCAAATTCCAACATCAGAAAACTTACTCAAACTGAAATCTGTGCTTTGTTATGATGGCTATCT
TACTCCAGCCAATCAATTAAAAACCTCACATTGCATCGGTGCAAGCCATGTTCGTGATAATGTTGATCGCCATTTTAGCG
AACAAGAACAACAAGAAAACCAACAAAAACTCCAACAAAATATCGCCCAACCTTGGACTCAAGATGTGAATACATCAGAT
AATCTCGCTCGTGTAGGCATTCGTTGCTCCGTAAGAGATCTTGCCCCAATGGTGGGAAACGTGCCTCATTTTGAACAGCA
ACAAGCGGATTATTACAATCTATTTAACCTCCGCCGCCGTAAACAACCTATTCAAAGTGCGATCAATTTTGAAAATCTTT
TCTTAACGGCAGCCTTAGGTTCTCGCGGGCTTACCTCTGCTCCACTATTAGGCGAAACCTTAGCTTCAATCATTTATGGC
GAGCCATTACCAATAAGCGAAGGCATATTACATAATCTTTCAGCTAACCGAGCTTGGGTAAAAAAGTGGTTGAAAGGTTC
AAAAGTTGAATAA

Upstream 100 bases:

>100_bases
ACTACGAAATAAAAATATTTCAAGCAATAATTCATTTGTTCGACCAGTTCAGAAAATTGCGTTATTATACGCAAATTTCA
TTAGGCAAGAAAGGAATAAA

Downstream 100 bases:

>100_bases
ATTCATAAGATTCAGAGCCTTACAAAAATAACTTTAACGTGTTAAAATCAAACAAAATTTAAATATAAAAATGAATACAA
AAATGCTATGCAATCAATCA

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MTFSVQHAEIHFNQNHIPVSDQFDDVYFSNENGLAETDYVFLQGNQLWERWITHKEANFVIAETGFGTGLNFFAVTKLFR
EFRQQHENHPLKRLNFISFEKYPLKITALLQAHLAYPQFEDLSAHLQRYWPSLILGCHRIHFGETTLDLWLGDVSENLPQ
LGDYMNERIDAWFLDGFAPSKNPEMWNDDLYNLMFRFTKPNGSFATFTAASAVRKGLESAGFNVTKRKGFGKKRECLSGL
KIQSKSTALSTPWYLAQPAKMEQQDVAIIGGGIASLCAAISLVKRGAKVTIYCEDDSLALNASGNKQGAFYPQLSDDNAL
TVDFYLHAFSYGRQLLDWAIAQNIAFEHEFCGVALCAYNKKSAVKLAKISQLGLPNEIFQMLNAEQLSEKVGLPLNCKGG
WIEQGAWLAPRQFVQNAFSFLEKQGVIIKTSQKITALSQLEKGWELKNMQGQKYCHEVVILANGYKITDFVQTEKLPLYP
IRGQVSQIPTSENLLKLKSVLCYDGYLTPANQLKTSHCIGASHVRDNVDRHFSEQEQQENQQKLQQNIAQPWTQDVNTSD
NLARVGIRCSVRDLAPMVGNVPHFEQQQADYYNLFNLRRRKQPIQSAINFENLFLTAALGSRGLTSAPLLGETLASIIYG
EPLPISEGILHNLSANRAWVKKWLKGSKVE

Sequences:

>Translated_670_residues
MTFSVQHAEIHFNQNHIPVSDQFDDVYFSNENGLAETDYVFLQGNQLWERWITHKEANFVIAETGFGTGLNFFAVTKLFR
EFRQQHENHPLKRLNFISFEKYPLKITALLQAHLAYPQFEDLSAHLQRYWPSLILGCHRIHFGETTLDLWLGDVSENLPQ
LGDYMNERIDAWFLDGFAPSKNPEMWNDDLYNLMFRFTKPNGSFATFTAASAVRKGLESAGFNVTKRKGFGKKRECLSGL
KIQSKSTALSTPWYLAQPAKMEQQDVAIIGGGIASLCAAISLVKRGAKVTIYCEDDSLALNASGNKQGAFYPQLSDDNAL
TVDFYLHAFSYGRQLLDWAIAQNIAFEHEFCGVALCAYNKKSAVKLAKISQLGLPNEIFQMLNAEQLSEKVGLPLNCKGG
WIEQGAWLAPRQFVQNAFSFLEKQGVIIKTSQKITALSQLEKGWELKNMQGQKYCHEVVILANGYKITDFVQTEKLPLYP
IRGQVSQIPTSENLLKLKSVLCYDGYLTPANQLKTSHCIGASHVRDNVDRHFSEQEQQENQQKLQQNIAQPWTQDVNTSD
NLARVGIRCSVRDLAPMVGNVPHFEQQQADYYNLFNLRRRKQPIQSAINFENLFLTAALGSRGLTSAPLLGETLASIIYG
EPLPISEGILHNLSANRAWVKKWLKGSKVE
>Mature_669_residues
TFSVQHAEIHFNQNHIPVSDQFDDVYFSNENGLAETDYVFLQGNQLWERWITHKEANFVIAETGFGTGLNFFAVTKLFRE
FRQQHENHPLKRLNFISFEKYPLKITALLQAHLAYPQFEDLSAHLQRYWPSLILGCHRIHFGETTLDLWLGDVSENLPQL
GDYMNERIDAWFLDGFAPSKNPEMWNDDLYNLMFRFTKPNGSFATFTAASAVRKGLESAGFNVTKRKGFGKKRECLSGLK
IQSKSTALSTPWYLAQPAKMEQQDVAIIGGGIASLCAAISLVKRGAKVTIYCEDDSLALNASGNKQGAFYPQLSDDNALT
VDFYLHAFSYGRQLLDWAIAQNIAFEHEFCGVALCAYNKKSAVKLAKISQLGLPNEIFQMLNAEQLSEKVGLPLNCKGGW
IEQGAWLAPRQFVQNAFSFLEKQGVIIKTSQKITALSQLEKGWELKNMQGQKYCHEVVILANGYKITDFVQTEKLPLYPI
RGQVSQIPTSENLLKLKSVLCYDGYLTPANQLKTSHCIGASHVRDNVDRHFSEQEQQENQQKLQQNIAQPWTQDVNTSDN
LARVGIRCSVRDLAPMVGNVPHFEQQQADYYNLFNLRRRKQPIQSAINFENLFLTAALGSRGLTSAPLLGETLASIIYGE
PLPISEGILHNLSANRAWVKKWLKGSKVE

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=671, Percent_Identity=45.3055141579732, Blast_Score=593, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 75696; Mature: 75565

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSVQHAEIHFNQNHIPVSDQFDDVYFSNENGLAETDYVFLQGNQLWERWITHKEANFV
CCEEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHCCCCCCEE
IAETGFGTGLNFFAVTKLFREFRQQHENHPLKRLNFISFEKYPLKITALLQAHLAYPQFE
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCHHHHHHHHHHCCCCHH
DLSAHLQRYWPSLILGCHRIHFGETTLDLWLGDVSENLPQLGDYMNERIDAWFLDGFAPS
HHHHHHHHHHHHHHHHHHHEECCCHHHHHEEHHHHHHHHHHHHHHHHHCCEEEECCCCCC
KNPEMWNDDLYNLMFRFTKPNGSFATFTAASAVRKGLESAGFNVTKRKGFGKKRECLSGL
CCCCCCCCHHHEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHCCC
KIQSKSTALSTPWYLAQPAKMEQQDVAIIGGGIASLCAAISLVKRGAKVTIYCEDDSLAL
EECCCCCCCCCCCEECCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEEE
NASGNKQGAFYPQLSDDNALTVDFYLHAFSYGRQLLDWAIAQNIAFEHEFCGVALCAYNK
ECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
KSAVKLAKISQLGLPNEIFQMLNAEQLSEKVGLPLNCKGGWIEQGAWLAPRQFVQNAFSF
CHHHHHHHHHHCCCCHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LEKQGVIIKTSQKITALSQLEKGWELKNMQGQKYCHEVVILANGYKITDFVQTEKLPLYP
HHHCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHEEEEEEECCEEEEHHHCCCCCCEEE
IRGQVSQIPTSENLLKLKSVLCYDGYLTPANQLKTSHCIGASHVRDNVDRHFSEQEQQEN
CCCCHHCCCCCHHHHHHHHHHHCCCCCCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHH
QQKLQQNIAQPWTQDVNTSDNLARVGIRCSVRDLAPMVGNVPHFEQQQADYYNLFNLRRR
HHHHHHHHHCCHHCCCCCCCCCEEECEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KQPIQSAINFENLFLTAALGSRGLTSAPLLGETLASIIYGEPLPISEGILHNLSANRAWV
HHHHHHHCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHH
KKWLKGSKVE
HHHHCCCCCC
>Mature Secondary Structure 
TFSVQHAEIHFNQNHIPVSDQFDDVYFSNENGLAETDYVFLQGNQLWERWITHKEANFV
CEEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHCCCCCCEE
IAETGFGTGLNFFAVTKLFREFRQQHENHPLKRLNFISFEKYPLKITALLQAHLAYPQFE
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCHHHHHHHHHHCCCCHH
DLSAHLQRYWPSLILGCHRIHFGETTLDLWLGDVSENLPQLGDYMNERIDAWFLDGFAPS
HHHHHHHHHHHHHHHHHHHEECCCHHHHHEEHHHHHHHHHHHHHHHHHCCEEEECCCCCC
KNPEMWNDDLYNLMFRFTKPNGSFATFTAASAVRKGLESAGFNVTKRKGFGKKRECLSGL
CCCCCCCCHHHEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHCCC
KIQSKSTALSTPWYLAQPAKMEQQDVAIIGGGIASLCAAISLVKRGAKVTIYCEDDSLAL
EECCCCCCCCCCCEECCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEEE
NASGNKQGAFYPQLSDDNALTVDFYLHAFSYGRQLLDWAIAQNIAFEHEFCGVALCAYNK
ECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
KSAVKLAKISQLGLPNEIFQMLNAEQLSEKVGLPLNCKGGWIEQGAWLAPRQFVQNAFSF
CHHHHHHHHHHCCCCHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LEKQGVIIKTSQKITALSQLEKGWELKNMQGQKYCHEVVILANGYKITDFVQTEKLPLYP
HHHCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHEEEEEEECCEEEEHHHCCCCCCEEE
IRGQVSQIPTSENLLKLKSVLCYDGYLTPANQLKTSHCIGASHVRDNVDRHFSEQEQQEN
CCCCHHCCCCCHHHHHHHHHHHCCCCCCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHH
QQKLQQNIAQPWTQDVNTSDNLARVGIRCSVRDLAPMVGNVPHFEQQQADYYNLFNLRRR
HHHHHHHHHCCHHCCCCCCCCCEEECEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KQPIQSAINFENLFLTAALGSRGLTSAPLLGETLASIIYGEPLPISEGILHNLSANRAWV
HHHHHHHCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHH
KKWLKGSKVE
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA