Click here to switch to the map view.

The map label for this gene is trpB [H]

Identifier: 148825971

GI number: 148825971

Start: 973929

End: 975122

Strand: Direct

Name: trpB [H]

Synonym: CGSHiEE_04740

Alternate gene names: 148825971

Gene position: 973929-975122 (Clockwise)

Preceding gene: 148825968

Following gene: 148825972

Centisome position: 53.72

GC content: 42.29

Gene sequence:

>1194_bases
ATGTCAGACACACTTCTTAACCCTTATTTTGGCGAATTTGGCGGAATGTATGTACCCGAAATTTTAGTGCCTGTTTTAAA
GCAATTAGAACAAGCCTTTGTGGAGGCTCAAAATGATCCTACATTCCAAGCAGAATTTGCTGATTTACTCAAAAATTATG
CGGGCAGACCGACCGCACTTACTCTTTGTCGTAACTTAACCAAAGGTACAAAAACTAAACTTTATCTTAAACGTGAAGAT
TTATTACACGGTGGTGCACACAAAACCAACCAAGTGTTAGGGCAAATTTTATTGGCTAAACGTATGGGCAAAACGCGTAT
TATTGCCGAAACAGGTGCAGGGCAACACGGCGTGGCAACAGCCCTAGCTTGTGCTATGTTAGATATGCCGTGCCGTGTTT
ATATGGGGGCAAAAGATGTTGAGCGTCAATCACCAAACGTGTTCCGTATGCGTTTAATGGGCGCGGAAGTGATTCCTGTA
CAAAAAGGCTCTTGTTCTTTAAAAGATGCCTGTTGTGAAGCGATGCGTGATTGGTCAGCAAACTATGAAACTACCCATTA
TTTACTTGGAACGGCGGCAGGCCCTCACCCCTTCCCGACGATTGTGAAAGAATTTCAAAAAATGATTGGCGAAGAAACCA
AACGTCAAATTCTCGAACGGGAAGGTCGTTTACCTGATGCAGTCATTGCTGCTGTTGGCGGTGGCTCGAATGCAATTGGT
ATGTTTGCTGATTTTATTGATGAATCCAATGTGCGTTTAATTGGTGTTGAGCCTGCGGGTAAAGGTATTGAAACGGGCGA
ACACGGTGCACCGCTCAAGCACGGCACAACAGGCATCTACTTCGGAATGAAATCGCCAATTATGCAAGATAAAGATGGAC
AAATTGAGGAATCCTATTCCATTTCTGCAGGGTTAGATTTTCCCTCTGTCGGGCCGCAACATGCTTATTTAAATGAGATT
GGTCGTGCTGATTATGTATCGATTACCGATGAAGAAGCCCTTAACGCATTCCAAGAATTGGCAAAACACGAAGGTATTAT
TCCTGCATTAGAAAGCTCACACGCCTTGGCTTATGCCTTGAAATTGATTAAACAAAATCCTGAAAAAGAACAACTTTTAG
TGGTTAATCTTTCAGGACGAGGCGATAAAGATATTTTTACCGTTGATAAAATTTTAAATGGAGGAACGAACTAA

Upstream 100 bases:

>100_bases
CCTGAACACGTGAACATTAATCGTATTGAAGTAATGCCAACCGCTCAATCTTTTGCAGGAATGAGCATTTCAAAAGAAAA
ATAAAATAATAGGAAAAAAT

Downstream 100 bases:

>100_bases
TGAGCCGTTTTGAAACGCAATTTGCGACACTTAACGCAAAAAATGAAGGGGCTTTCGTGCCGTTCGTTACCCTTTGCGAC
CCTACTTTCGACCGCTCTTT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSDTLLNPYFGEFGGMYVPEILVPVLKQLEQAFVEAQNDPTFQAEFADLLKNYAGRPTALTLCRNLTKGTKTKLYLKRED
LLHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVATALACAMLDMPCRVYMGAKDVERQSPNVFRMRLMGAEVIPV
QKGSCSLKDACCEAMRDWSANYETTHYLLGTAAGPHPFPTIVKEFQKMIGEETKRQILEREGRLPDAVIAAVGGGSNAIG
MFADFIDESNVRLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNEI
GRADYVSITDEEALNAFQELAKHEGIIPALESSHALAYALKLIKQNPEKEQLLVVNLSGRGDKDIFTVDKILNGGTN

Sequences:

>Translated_397_residues
MSDTLLNPYFGEFGGMYVPEILVPVLKQLEQAFVEAQNDPTFQAEFADLLKNYAGRPTALTLCRNLTKGTKTKLYLKRED
LLHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVATALACAMLDMPCRVYMGAKDVERQSPNVFRMRLMGAEVIPV
QKGSCSLKDACCEAMRDWSANYETTHYLLGTAAGPHPFPTIVKEFQKMIGEETKRQILEREGRLPDAVIAAVGGGSNAIG
MFADFIDESNVRLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNEI
GRADYVSITDEEALNAFQELAKHEGIIPALESSHALAYALKLIKQNPEKEQLLVVNLSGRGDKDIFTVDKILNGGTN
>Mature_396_residues
SDTLLNPYFGEFGGMYVPEILVPVLKQLEQAFVEAQNDPTFQAEFADLLKNYAGRPTALTLCRNLTKGTKTKLYLKREDL
LHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVATALACAMLDMPCRVYMGAKDVERQSPNVFRMRLMGAEVIPVQ
KGSCSLKDACCEAMRDWSANYETTHYLLGTAAGPHPFPTIVKEFQKMIGEETKRQILEREGRLPDAVIAAVGGGSNAIGM
FADFIDESNVRLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNEIG
RADYVSITDEEALNAFQELAKHEGIIPALESSHALAYALKLIKQNPEKEQLLVVNLSGRGDKDIFTVDKILNGGTN

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=389, Percent_Identity=82.7763496143959, Blast_Score=679, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=385, Percent_Identity=52.2077922077922, Blast_Score=411, Evalue=1e-116,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43229; Mature: 43098

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLLNPYFGEFGGMYVPEILVPVLKQLEQAFVEAQNDPTFQAEFADLLKNYAGRPTAL
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHH
TLCRNLTKGTKTKLYLKREDLLHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVAT
HHHHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
ALACAMLDMPCRVYMGAKDVERQSPNVFRMRLMGAEVIPVQKGSCSLKDACCEAMRDWSA
HHHHHHHCCCHHEEECCHHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCC
NYETTHYLLGTAAGPHPFPTIVKEFQKMIGEETKRQILEREGRLPDAVIAAVGGGSNAIG
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCHHH
MFADFIDESNVRLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQDKDGQIEESYS
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCE
ISAGLDFPSVGPQHAYLNEIGRADYVSITDEEALNAFQELAKHEGIIPALESSHALAYAL
EECCCCCCCCCCCHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KLIKQNPEKEQLLVVNLSGRGDKDIFTVDKILNGGTN
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SDTLLNPYFGEFGGMYVPEILVPVLKQLEQAFVEAQNDPTFQAEFADLLKNYAGRPTAL
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHH
TLCRNLTKGTKTKLYLKREDLLHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVAT
HHHHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
ALACAMLDMPCRVYMGAKDVERQSPNVFRMRLMGAEVIPVQKGSCSLKDACCEAMRDWSA
HHHHHHHCCCHHEEECCHHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCC
NYETTHYLLGTAAGPHPFPTIVKEFQKMIGEETKRQILEREGRLPDAVIAAVGGGSNAIG
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCHHH
MFADFIDESNVRLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQDKDGQIEESYS
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCE
ISAGLDFPSVGPQHAYLNEIGRADYVSITDEEALNAFQELAKHEGIIPALESSHALAYAL
EECCCCCCCCCCCHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KLIKQNPEKEQLLVVNLSGRGDKDIFTVDKILNGGTN
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA