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The map label for this gene is glgX [H]

Identifier: 148825897

GI number: 148825897

Start: 897931

End: 899907

Strand: Direct

Name: glgX [H]

Synonym: CGSHiEE_04320

Alternate gene names: 148825897

Gene position: 897931-899907 (Clockwise)

Preceding gene: 148825896

Following gene: 148825898

Centisome position: 49.53

GC content: 42.29

Gene sequence:

>1977_bases
ATGTTCAAAATCTACAACAACGGCAATCCTATTCCAATGGGATACTCACAAGCGGTCGAAAACAACGTGCAAATTACCAA
TTTCGCCCTGTTTTCCGCTGCGGCAACAGGCGTGGAACTTTGCCTATTTGCCGAACAAAATCAAGAAACTCGCTTGCCAA
TGGTGCGTACTGAAAATATGTGGCATTTGGCGGTAACTGGCGTGAAAACTGGTACGGAATATGGTTTTCGTATTCACGGC
GAATTTGCCAATCCGCAGAAATTGATACTCGATCCCTATGCGAAAGCCGTGAATGGTAAACCGGATTTAACTACCGAAGA
AAGTCGTTCTTGGTTTTTAATGAATGATCATCGCGATAATGCTCATATTGCGCCAAGAGCGGTGATTATTTCTGAAGATT
TTGATTGGGAAAATGATACCTCCCCCAATACGCCTTGGGCTGAAACCATTGTGTATGAATTACATGCTAAAGGGTTTAGC
CAGTTAAACGAGAAAATCCCTGCGGCGTTGCGTGGTACTTATACTGGATTAGCTCATCCTGTGAATTTGGCGTATTTGAA
AGAATTAGGCGTAACTGCGGTGGAGTTGCTGCCTGTAAATTTCCATATAAATGAACCGAACTTACAGGCACGAGGCTTAC
AAAATTATTGGGGATACAATCCCTTAGCGATGTTTGCGGTAGAACCTAAATATGCGGCAACAAATAATCCATTAGCTGAA
TTTAAAGCGATGGTAAAGGCGTTTCACAATGCGGGCATTGAAGTTATTTTAGATGTGGTGTTTAACCATTCCGCAGAATC
AGAGCAAACTTACCCAACATTCAGCCAGCGTGGTATTGATGATCAAACTTACTATTGGCGCAACGATCAAGGGCGTTATA
TCAATTGGACAGGCTGCGGCAATATGCTCAATTTATCCTCTGATGTAGGGCGAAAGTGGGTGGTGGATTGCCTGCGTTAT
TGGGTGGAGCAATGCCACATTGATGGTTTCCGTTTTGATTTAGCCTCGGTGCTTGGGCGTAATACACCTGATTTCAATTC
GTCAGCTCAGCTTTTTACTGACATAAAAAATGAGCCAAGTTTACAAAATATTAAATTGATTGCCGAGCCTTGGGATATTG
GACATTACGGTTATCAAGTCGGCAATTTCCCAAGTTATTTTGCCGAATGGAACGACCGATTCCGCGATGATCTCTGCCGT
TTCTGGCTATGGAAAAGTGGCGAAATTGGTGCATTTGCAGAACGTTTTGCAGGTTCTAGCGATTTATTTAAGAAAAATGA
CCGCTTGCCACATACTACACTTAATTTTATTACCGCTCACGATGGTTTTACGCTTAAAGATTTAGTGAGTTACAACCAAA
AACATAATGAAGCGAACGGCGAAGAAAATCACGACGGCAGAAACGAAAACTACAGCTACAACCACGGCATAGAGGGTTCG
ACTGAGAATCTTGCTGAGCCACAAAAAAGTGCGGTGGAAAATAACCGCACTTTTGCACAAAGCGGATTATTAATGAGTTT
ATTGCTGGCGAATGGTACACCTATGTTGTTAGCTGGCGATGAGTTCGGCAACACTCAATATGGTAACAATAATGCTTATT
GCCAAGATAACGAGATTACTTGGCTGAAATGGGCAAATTTTAACGAAGAATTATTTGAACTGACCAAACAAACGATTGCA
CTTCGTAAGCAAATTGGCAGTTTAAACCGAGATCAATGGTGGTCGGATGAGAATGTGCAATGGCTTAACATTGCAGGCGA
ACCGATGACAGTTGAAGATTGGCAAAATCAACAAACCAAAGCATTACAGGTGGTTTTAGACAATCGTTGGCTTTTGTTGA
TTAATGCCAAAGCCGAAGGTCAGGTGTTTCACTTGCCTAATGGGAAATGGAAGCCCCAAATCGGCACACATAATGTAACC
CTTGAAGAACAAGCCGAACTTAGTTCAATGGGCTTTTGTATGCTGAATGACGAATAA

Upstream 100 bases:

>100_bases
AATCCGAATAGATAAATTGTAGGGGCGAATTACATTCGCCCTGATAAACCACCGATGTTTTTGGGCAAATGTAATTTGCC
CCTACAAAAGCGATAAAAAT

Downstream 100 bases:

>100_bases
CAATAAGACTATGGAGATCCTTATGAAATCTGGCGACCTTAACAAATATGATTTAGTTAAAAACACTTTGGTGCTTGTTC
TCGCAGGTGGACGAGGTTCA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAATGVELCLFAEQNQETRLPMVRTENMWHLAVTGVKTGTEYGFRIHG
EFANPQKLILDPYAKAVNGKPDLTTEESRSWFLMNDHRDNAHIAPRAVIISEDFDWENDTSPNTPWAETIVYELHAKGFS
QLNEKIPAALRGTYTGLAHPVNLAYLKELGVTAVELLPVNFHINEPNLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
FKAMVKAFHNAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCGNMLNLSSDVGRKWVVDCLRY
WVEQCHIDGFRFDLASVLGRNTPDFNSSAQLFTDIKNEPSLQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCR
FWLWKSGEIGAFAERFAGSSDLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNEANGEENHDGRNENYSYNHGIEGS
TENLAEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEITWLKWANFNEELFELTKQTIA
LRKQIGSLNRDQWWSDENVQWLNIAGEPMTVEDWQNQQTKALQVVLDNRWLLLINAKAEGQVFHLPNGKWKPQIGTHNVT
LEEQAELSSMGFCMLNDE

Sequences:

>Translated_658_residues
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAATGVELCLFAEQNQETRLPMVRTENMWHLAVTGVKTGTEYGFRIHG
EFANPQKLILDPYAKAVNGKPDLTTEESRSWFLMNDHRDNAHIAPRAVIISEDFDWENDTSPNTPWAETIVYELHAKGFS
QLNEKIPAALRGTYTGLAHPVNLAYLKELGVTAVELLPVNFHINEPNLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
FKAMVKAFHNAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCGNMLNLSSDVGRKWVVDCLRY
WVEQCHIDGFRFDLASVLGRNTPDFNSSAQLFTDIKNEPSLQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCR
FWLWKSGEIGAFAERFAGSSDLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNEANGEENHDGRNENYSYNHGIEGS
TENLAEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEITWLKWANFNEELFELTKQTIA
LRKQIGSLNRDQWWSDENVQWLNIAGEPMTVEDWQNQQTKALQVVLDNRWLLLINAKAEGQVFHLPNGKWKPQIGTHNVT
LEEQAELSSMGFCMLNDE
>Mature_658_residues
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAATGVELCLFAEQNQETRLPMVRTENMWHLAVTGVKTGTEYGFRIHG
EFANPQKLILDPYAKAVNGKPDLTTEESRSWFLMNDHRDNAHIAPRAVIISEDFDWENDTSPNTPWAETIVYELHAKGFS
QLNEKIPAALRGTYTGLAHPVNLAYLKELGVTAVELLPVNFHINEPNLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
FKAMVKAFHNAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCGNMLNLSSDVGRKWVVDCLRY
WVEQCHIDGFRFDLASVLGRNTPDFNSSAQLFTDIKNEPSLQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCR
FWLWKSGEIGAFAERFAGSSDLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNEANGEENHDGRNENYSYNHGIEGS
TENLAEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEITWLKWANFNEELFELTKQTIA
LRKQIGSLNRDQWWSDENVQWLNIAGEPMTVEDWQNQQTKALQVVLDNRWLLLINAKAEGQVFHLPNGKWKPQIGTHNVT
LEEQAELSSMGFCMLNDE

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=668, Percent_Identity=49.4011976047904, Blast_Score=639, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75013; Mature: 75013

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAATGVELCLFAEQNQETRLPMVRTENM
CEEEECCCCCCCCCHHHHHCCCCEEEEEEHHHHHHCCEEEEEEECCCCCCCCCEEEECCE
WHLAVTGVKTGTEYGFRIHGEFANPQKLILDPYAKAVNGKPDLTTEESRSWFLMNDHRDN
EEEEEEEEECCCCCCEEEECCCCCCCEEEECCHHHHCCCCCCCCCCCCCCEEEEECCCCC
AHIAPRAVIISEDFDWENDTSPNTPWAETIVYELHAKGFSQLNEKIPAALRGTYTGLAHP
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
VNLAYLKELGVTAVELLPVNFHINEPNLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
CHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCHHH
FKAMVKAFHNAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCG
HHHHHHHHHHCCHHEEHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCEEEEECCC
NMLNLSSDVGRKWVVDCLRYWVEQCHIDGFRFDLASVLGRNTPDFNSSAQLFTDIKNEPS
CEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
LQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCRFWLWKSGEIGAFAERFAGSS
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
DLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNEANGEENHDGRNENYSYNHGIEGS
HHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TENLAEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEIT
CHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCEE
WLKWANFNEELFELTKQTIALRKQIGSLNRDQWWSDENVQWLNIAGEPMTVEDWQNQQTK
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCHHE
ALQVVLDNRWLLLINAKAEGQVFHLPNGKWKPQIGTHNVTLEEQAELSSMGFCMLNDE
EEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHCCEEEECCC
>Mature Secondary Structure
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAATGVELCLFAEQNQETRLPMVRTENM
CEEEECCCCCCCCCHHHHHCCCCEEEEEEHHHHHHCCEEEEEEECCCCCCCCCEEEECCE
WHLAVTGVKTGTEYGFRIHGEFANPQKLILDPYAKAVNGKPDLTTEESRSWFLMNDHRDN
EEEEEEEEECCCCCCEEEECCCCCCCEEEECCHHHHCCCCCCCCCCCCCCEEEEECCCCC
AHIAPRAVIISEDFDWENDTSPNTPWAETIVYELHAKGFSQLNEKIPAALRGTYTGLAHP
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
VNLAYLKELGVTAVELLPVNFHINEPNLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
CHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCHHH
FKAMVKAFHNAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCG
HHHHHHHHHHCCHHEEHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCEEEEECCC
NMLNLSSDVGRKWVVDCLRYWVEQCHIDGFRFDLASVLGRNTPDFNSSAQLFTDIKNEPS
CEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
LQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCRFWLWKSGEIGAFAERFAGSS
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
DLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNEANGEENHDGRNENYSYNHGIEGS
HHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TENLAEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEIT
CHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCEE
WLKWANFNEELFELTKQTIALRKQIGSLNRDQWWSDENVQWLNIAGEPMTVEDWQNQQTK
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCHHE
ALQVVLDNRWLLLINAKAEGQVFHLPNGKWKPQIGTHNVTLEEQAELSSMGFCMLNDE
EEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]