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The map label for this gene is glnS [H]

Identifier: 148825894

GI number: 148825894

Start: 891268

End: 892938

Strand: Direct

Name: glnS [H]

Synonym: CGSHiEE_04295

Alternate gene names: 148825894

Gene position: 891268-892938 (Clockwise)

Preceding gene: 148825893

Following gene: 148825895

Centisome position: 49.16

GC content: 40.1

Gene sequence:

>1671_bases
ATGAGCCATACAGAGACATCTTTGGGTGCAGAAAATACCCGCACTCATAATTTTATTACCCAAATTATTGATGAAGATTT
AGCTTCGGGTAAACATAAAAGCGTTCATACCCGTTTTCCGCCAGAGCCGAATGGCTATTTGCATATTGGTCACGCAAAGT
CTATTTGCTTGAATTTTGGCTTGGCAAAAGAATATCAAGGTTTATGCAACCTACGTTTTGACGATACCAATCCTGTAAAA
GAAGATGTGGAATATGTGGATTCCATCAAAGCTGACGTGGAATGGTTAGGTTTCAAATGGGAGGGCGAACCGCGTTATGC
ATCTGATTACTTCGATGCGCTTTATGGCTATGCGATTGAATTGATTAAAAAAGGTTTGGCTTATGTTGATGAACTTTCGC
CAGATGAAATGCGTGAATATCGTGGTACCTTAACCGAACCTGGTAAAAACAGCCCATATCGTGACCGCACTATTGAAGAA
AACTTAGCGTTATTTGAAAAAATGAAGAACGGTGAGTTTGCAGAGGGGAAAGCAAGTTTACGTGCAAAAATTGATATGGC
ATCGCCATTTATGGTCATGCGTGATCCAGTAATTTATCGTATTAAATTCGCTAGCCATCACCAAACAGGTGACAAATGGT
GTATTTACCCAATGTACGATTTCACTCACTGTATCTCTGATGCCATTGAGCGTATTACACACTCAATCTGTACATTAGAA
TTCCAAGATAACCGTCGTTTATATGACTGGGTGTTGGAAAATATCAGTATTGAGCGTCCATTACCGCATCAATATGAATT
TTCACGTTTAAATTTAGAAGGCACTTTAACCTCTAAACGTAAATTATTGAAATTGGTAAATGATGAAATCGTGGATGGTT
GGAACGATCCCCGTATGCCAACCATTTCAGGTTTACGCCGTCGTGGTTATACTCCAGCTTCATTGCGTGAATTCTGCCGT
CGTATTGGTGTGACTAAACAAGATAACGTCGTAGAGTATTCAGCACTTGAAGCCTGCATTCGTGAGGATTTAAATGAAAA
TGCACCACGCGCAATGGCGGTGATTGATCCTGTTCGTGTGGTAATTGAAAATTTTGAAAGCGAAGCGGTTTTAACTGCCC
CAAATCATCCAAATCGTCCTGAATTAGGGGAGCGTCAATTGCCGTTCACGAAAGAGCTTTATATTGATCGCACAGACTTC
CGTGAGGAAGCGAACAAACAATATAAACGCTTAGTATTAGGCAAAGAAGTGCGTTTGCGTAACGCTTATGTGATTAAAGC
GGAACGTGTAGAAAAAGATGCAAGTGGCGAAATCACCACGATTTTCTGTACTTACGATCCTGAAACTTTAGGTAAAAATC
CAGCAGATGGACGTAAAGTAAAAGGTGTCATTCATTGGGTTTCTGCGGTTAATAATCATCCAGCTGAATTCCGTTTGTAT
GATCGCTTATTTACTGTGCCAAATCCAGGGGCTGAAGATGATATCGAAAGCGTGTTAAACCCAAATTCTTTAGTGATAAA
ACAAGGTTTTGTAGAGCAAAGCCTCGCAAATGCAGAAGCAGAGAAAGGTTACCAATTTGAACGTGAGGGTTATTTCTGTG
CAGATAGTAAAGATAGCCGTCCTGAACATTTGGTATTTAACTTAACAGTAAGCTTAAAAGAAGGCTTCTAA

Upstream 100 bases:

>100_bases
AATCTAGTATTTCTATGGCAAGAACAGGAGATGAACCTTTATTTTCCCTTAGATTTGTATAGAATAGCCGAATATTTTTT
TATTTTAACGAGAGAAAATG

Downstream 100 bases:

>100_bases
TTTTAATCAACCAAGTGCGGTCAAAACAAACGTGATTTTGACCGCACTTTTTATTTGTATCAACTATGCAATCCAATATG
CAACTTGAACCCAATTTCTG

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLAKEYQGLCNLRFDDTNPVK
EDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAIELIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIEE
NLALFEKMKNGEFAEGKASLRAKIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLE
FQDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRMPTISGLRRRGYTPASLREFCR
RIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRTDF
REEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRLY
DRLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDSRPEHLVFNLTVSLKEGF

Sequences:

>Translated_556_residues
MSHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLAKEYQGLCNLRFDDTNPVK
EDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAIELIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIEE
NLALFEKMKNGEFAEGKASLRAKIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLE
FQDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRMPTISGLRRRGYTPASLREFCR
RIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRTDF
REEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRLY
DRLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDSRPEHLVFNLTVSLKEGF
>Mature_555_residues
SHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLAKEYQGLCNLRFDDTNPVKE
DVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAIELIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIEEN
LALFEKMKNGEFAEGKASLRAKIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLEF
QDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRMPTISGLRRRGYTPASLREFCRR
IGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRTDFR
EEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRLYD
RLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDSRPEHLVFNLTVSLKEGF

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=530, Percent_Identity=47.7358490566038, Blast_Score=447, Evalue=1e-126,
Organism=Homo sapiens, GI62241042, Length=517, Percent_Identity=33.2688588007737, Blast_Score=270, Evalue=3e-72,
Organism=Escherichia coli, GI1786895, Length=546, Percent_Identity=76.9230769230769, Blast_Score=889, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=303, Percent_Identity=22.4422442244224, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543332, Length=562, Percent_Identity=40.3914590747331, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17511007, Length=521, Percent_Identity=32.0537428023033, Blast_Score=245, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6324742, Length=563, Percent_Identity=41.740674955595, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321192, Length=523, Percent_Identity=35.5640535372849, Blast_Score=309, Evalue=9e-85,
Organism=Drosophila melanogaster, GI17864488, Length=542, Percent_Identity=42.9889298892989, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24649466, Length=519, Percent_Identity=34.4894026974952, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 63935; Mature: 63804

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCC
LAKEYQGLCNLRFDDTNPVKEDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAIE
CHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIEENLALFEKMKNGEFAEGKASL
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
RAKIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLE
EEEEECCCCEEEECCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEE
FQDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRMP
ECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCC
TISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRV
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
VIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRTDFREEANKQYKRLVLGKEVRLR
HHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHEECHHHHHHHHHHHHHHHHHCCCEEEC
NAYVIKAERVEKDASGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRLY
CEEEEEHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
DRLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDSR
EEEEECCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHCCCEECCCCEEECCCCCCC
PEHLVFNLTVSLKEGF
CCEEEEEEEEEEECCC
>Mature Secondary Structure 
SHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCC
LAKEYQGLCNLRFDDTNPVKEDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAIE
CHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIEENLALFEKMKNGEFAEGKASL
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
RAKIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLE
EEEEECCCCEEEECCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEE
FQDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRMP
ECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCC
TISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRV
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
VIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRTDFREEANKQYKRLVLGKEVRLR
HHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHEECHHHHHHHHHHHHHHHHHCCCEEEC
NAYVIKAERVEKDASGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRLY
CEEEEEHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
DRLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDSR
EEEEECCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHCCCEECCCCEEECCCCCCC
PEHLVFNLTVSLKEGF
CCEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA