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The map label for this gene is nusA [H]

Identifier: 148825866

GI number: 148825866

Start: 856197

End: 857684

Strand: Direct

Name: nusA [H]

Synonym: CGSHiEE_04155

Alternate gene names: 148825866

Gene position: 856197-857684 (Clockwise)

Preceding gene: 148825865

Following gene: 148825867

Centisome position: 47.22

GC content: 38.51

Gene sequence:

>1488_bases
ATGAGTAAAGAAATTTTACTGGCGGCGGAAGCTGTATCCAATGAAAAATTATTACCACGTGAGAAAATTTTTGAAGCATT
AGAGAGTGCGATTGCGATTTCTACTAAGAAGAAGTTTGAATATGAAACGGATATTCGCGTTTCAATTAATCCCAAAACAG
GAGAATTTGATACTTTTCGCCGTTGGTTAGTCGTAGATGAAGTAAAAGTACCAACGAAGGAAATTACTTTGGAAGCGGCG
CAATTTGATGATCCAAATTTGCAACTTGGAGAGTATGTAGAAGATCAAATTGAATCTATTGCATTTGACCGTATTGCAAT
GCAAACAGCACGTCAAGTTATTAGCACTAAAATTCGTGAAGCCGAACGTGCAAAAGTGGTTGAACAATTTCGTGAAGAAG
AAGGAAAAATTGTAACAGGCACTGTAAAAAAAGTAAGTCGAGACAGTATTATTTTAGATCTTGGCAATAAAGCGGAAGCG
ATGATTGCACGTGAAGATATGTTACCACGTGAAAACTTCCGTCCGGGAGATCGTGTTCGTGGGGTGCTTTATAAAGTAAA
TCCTGAAGGCAAAACTGCACAATTATTTGTGACACGTTCTAAACCAGAAATGTTGATCGAATTATTCCGTATTGAAGTGC
CTGAAATTGGCGAAGAAATGATTGAAATTCGTGGTGCAGCTCGTGATGCTGGCTCTCGCGCTAAAATTGCAGTGAAATCA
AATGATAAGCGAATTGATCCAGTTGGTGCTTGTGTAGGAATGCGTGGTGCGCGTGTTCAAGCCATTACCAATGAACTTGG
TGGTGAGCGTGTAGATATCGTGCTTTGGGATGATAATCCAGCACAATTTGTGATTAATGCGATGGCGCCTGCGGATGTTT
CTTCTATTATCGTTGATGAAGATAATCATTCTATGGATATTGCTGTTGAAGCTGATAATCTTGCTCAAGCCATTGGTCGT
AATGGTCAAAACGTGCGTTTAGCAACACAATTAACAGGTTGGACACTAAACGTAATGACGACTGATGAGTTAAATGCTAA
GCATCAAGCAGAAGATAACAAAGTGTTAAATTTATTTATTAACGCATTAGAACTTGATGAAGAATTTGCTCAAATCCTTG
TAGAAGAAGGCTTCACAAGTTTAGAAGAAATTGCTTATGTGCCAATGAATGAATTGACAGCAATTGATGGTTTGGAAGAT
GAAGATCTTGTTGAAGAACTTCAAACTCGTGCTAAAAATGCGATTACAGCCGCAGCAGTAGCTGAAGAAGAGGCATTGAA
AAAAGCAAATATTGAAGATCGCTTATTGAATTTAGATGGTATGAATCGCCATGTGGCATTCAGATTAGCTGAAAAACAAA
TTACTACTCTCGAAGAACTCGCCGAACAGGGTGTAGATGATTTAGCAGATATTGAAGAATTAACAGCAGAACAGGCAGCA
GATTTAATTATGGCTGCACGTAATATCTGTTGGTTTGGCGAAGAATAA

Upstream 100 bases:

>100_bases
ATGATTACTTTAATCGTTGATGATCAAGAACAAATTTTAGTCTTCGGAAACATTCAAAAAGCGAATGTTGTTGCAAAATT
TTAAAAGGAGAAAAAGAGAA

Downstream 100 bases:

>100_bases
GGAGAGTAGTAATGACTGAAGATGTTAAGGCTGATGCGCCAAAAAAAATTAAGCATTCAACGCAGAACAAAAACAACAGT
GAGCAGCACCACAACTGGCG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFRRWLVVDEVKVPTKEITLEAA
QFDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIREAERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEA
MIAREDMLPRENFRPGDRVRGVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKS
NDKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDEDNHSMDIAVEADNLAQAIGR
NGQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFINALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLED
EDLVEELQTRAKNAITAAAVAEEEALKKANIEDRLLNLDGMNRHVAFRLAEKQITTLEELAEQGVDDLADIEELTAEQAA
DLIMAARNICWFGEE

Sequences:

>Translated_495_residues
MSKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFRRWLVVDEVKVPTKEITLEAA
QFDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIREAERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEA
MIAREDMLPRENFRPGDRVRGVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKS
NDKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDEDNHSMDIAVEADNLAQAIGR
NGQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFINALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLED
EDLVEELQTRAKNAITAAAVAEEEALKKANIEDRLLNLDGMNRHVAFRLAEKQITTLEELAEQGVDDLADIEELTAEQAA
DLIMAARNICWFGEE
>Mature_494_residues
SKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFRRWLVVDEVKVPTKEITLEAAQ
FDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIREAERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEAM
IAREDMLPRENFRPGDRVRGVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKSN
DKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDEDNHSMDIAVEADNLAQAIGRN
GQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFINALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLEDE
DLVEELQTRAKNAITAAAVAEEEALKKANIEDRLLNLDGMNRHVAFRLAEKQITTLEELAEQGVDDLADIEELTAEQAAD
LIMAARNICWFGEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=495, Percent_Identity=73.3333333333333, Blast_Score=740, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55267; Mature: 55136

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFR
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
RWLVVDEVKVPTKEITLEAAQFDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIRE
HEEEEECCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEAMIAREDMLPRENFRPGDRVR
HHHHHHHHHHHHHCCCEEEEEHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHC
GVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKS
EEEEEECCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEC
NDKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEEECCCCCCCEEEEEC
DNHSMDIAVEADNLAQAIGRNGQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFI
CCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHH
NALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLEDEDLVEELQTRAKNAITAAAV
HHHHHHHHHHHHHHHHCCHHHHHHEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AEEEALKKANIEDRLLNLDGMNRHVAFRLAEKQITTLEELAEQGVDDLADIEELTAEQAA
HHHHHHHHCCCHHHEECCCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DLIMAARNICWFGEE
HHHHHHHHCEECCCC
>Mature Secondary Structure 
SKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFR
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
RWLVVDEVKVPTKEITLEAAQFDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIRE
HEEEEECCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEAMIAREDMLPRENFRPGDRVR
HHHHHHHHHHHHHCCCEEEEEHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHC
GVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKS
EEEEEECCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEC
NDKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEEECCCCCCCEEEEEC
DNHSMDIAVEADNLAQAIGRNGQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFI
CCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHH
NALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLEDEDLVEELQTRAKNAITAAAV
HHHHHHHHHHHHHHHHCCHHHHHHEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AEEEALKKANIEDRLLNLDGMNRHVAFRLAEKQITTLEELAEQGVDDLADIEELTAEQAA
HHHHHHHHCCCHHHEECCCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DLIMAARNICWFGEE
HHHHHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]