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The map label for this gene is kgd [H]

Identifier: 148825798

GI number: 148825798

Start: 770594

End: 771823

Strand: Direct

Name: kgd [H]

Synonym: CGSHiEE_03745

Alternate gene names: 148825798

Gene position: 770594-771823 (Clockwise)

Preceding gene: 148825797

Following gene: 148825806

Centisome position: 42.5

GC content: 40.24

Gene sequence:

>1230_bases
ATGACAATCGAAATTCTTGTTCCAGACCTACCTGAATCAGTTGCTGATGCAACTGTTGCTACTTGGCATAAAAAACTGGG
CGATACTGTGAAACGTGATGAGGTTATTGTGGAAATTGAAACGGATAAAGTCGTGCTAGAAGTTCCCGCACTTTCTGATG
GCGTGTTGGCAGAAGTTGTTCAAGCTGAGGGCAAAACAGTGGTGAGTAAACAGTTGCTAGGAAAAATTTCTACGGCTCAA
GAAGGAGATGTCAGTTCAGCGACGTTAAAAGCAACGAATGAACCCACACCATCAGATCGTCAAAATGCAGCGATTGAAAA
TAGTCATAATCATAATGCGGATCAGAGCCCTGCAATTCGTCGTTTATTAGCAGAACATGATTTACAAGCAGATCAAATTC
AAGGTTCAGGTGTGGGTGGTCGTTTAACACGTGAAGATATTGAACGTGAAATTGCAAAACGACAAGCACAGCAAGTGAAA
CAAGAAGCTGCCACTGAGCAAAATACAATTAGTACGGTGGCTTATAGCGCACGTTCTGAAAAACGTGTGCCAATGACCCG
TTTGCGTAAACGTATTGCTGAACGTTTACTTGAAGCCAAAAATAGTACGGCGATGCTTACCACTTTCAATGAAGTGGATA
TGCAGCCGATAATGACTCTGCGTAAAACCTACGGCGAAAAATTTGAAAAACAACATTCAGTACGTTTAGGATTTATGTCT
TTTTATATTAAAGCGGTTGTGGAAGCATTAAAACGTTATCCTGAAGTGAATGCTTCTATTGATGGGGATGATGTTGTTTA
CCATAACTATTTTGATATTAGTATTGCAGTTTCAACACCGCGTGGATTAGTCACACCAGTGCTTCGTGATTGCGATAAGC
TCAGTATGGCAGAGATCGAAAAACAAATTAAAGCATTAGCTGAAAAAGGTCGTGATGGCAAATTAACAGTAGAAGATCTT
ACTGGCGGCAATTTTACTATTACAAATGGTGGCGTGTTCGGTTCTCTTATGTCCACTCCAATTATCAATCCACCACAAAG
TGCGATTTTAGGAATGCACGCTATTAAAGAACGCCCGATTGCGCTTAATGGTCAAGTTGTGATTCGTCCTATGATGTACC
TTGCTTTATCTTACGATCATCGTTTAATTGACGGCCGTGAATCTGTTGGTTTCTTGGTAGCGATTAAAGAATTATTGGAA
GATCCAACAAGATTGTTATTAGAAATCTAA

Upstream 100 bases:

>100_bases
GTTAATTTAATAAAGGTTTTTATATCTTTGCTTTCCTCTGTAAATAGGGGAGCCAAGATAATTAAGATATAGAGTAAAAA
AGAAGAAAAGGAAAAATAAA

Downstream 100 bases:

>100_bases
GAAATAAAAGGCTTTCTGTGTTAGATGTTAGAAAGCCTTTTAAAATGAACAAAAAATTAACCGCACTTTAAATATTTAAA
AAGAATATTCCAAAGTGCGG

Product: alpha-ketoglutarate decarboxylase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVVQAEGKTVVSKQLLGKISTAQ
EGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIRRLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVK
QEAATEQNTISTVAYSARSEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMS
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIEKQIKALAEKGRDGKLTVEDL
TGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPIALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAIKELLE
DPTRLLLEI

Sequences:

>Translated_409_residues
MTIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVVQAEGKTVVSKQLLGKISTAQ
EGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIRRLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVK
QEAATEQNTISTVAYSARSEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMS
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIEKQIKALAEKGRDGKLTVEDL
TGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPIALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAIKELLE
DPTRLLLEI
>Mature_408_residues
TIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVVQAEGKTVVSKQLLGKISTAQE
GDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIRRLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVKQ
EAATEQNTISTVAYSARSEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMSF
YIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIEKQIKALAEKGRDGKLTVEDLT
GGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPIALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAIKELLED
PTRLLLEI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=58.5470085470086, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=28.3813747228381, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=28.6031042128603, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=28.0459770114943, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=26.5588914549654, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=36.3095238095238, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=73.4793187347932, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=29.1764705882353, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=402, Percent_Identity=41.7910447761194, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=30.3854875283447, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=25.2927400468384, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=28.9902280130293, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=405, Percent_Identity=42.4691358024691, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=25.7709251101322, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=60, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=27.906976744186, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=34.061135371179, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=34.061135371179, Blast_Score=138, Evalue=6e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45134; Mature: 45003

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVV
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHH
QAEGKTVVSKQLLGKISTAQEGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIR
HHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVKQEAATEQNTISTVAYSARSE
HHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMS
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCHHHHHHH
KQIKALAEKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPI
HHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
ALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAIKELLEDPTRLLLEI
EECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
TIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVV
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHH
QAEGKTVVSKQLLGKISTAQEGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIR
HHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVKQEAATEQNTISTVAYSARSE
HHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMS
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCHHHHHHH
KQIKALAEKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPI
HHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
ALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAIKELLEDPTRLLLEI
EECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]