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The map label for this gene is rfaB [C]

Identifier: 148825758

GI number: 148825758

Start: 722113

End: 723174

Strand: Direct

Name: rfaB [C]

Synonym: CGSHiEE_03545

Alternate gene names: 148825758

Gene position: 722113-723174 (Clockwise)

Preceding gene: 148825757

Following gene: 148825759

Centisome position: 39.83

GC content: 30.51

Gene sequence:

>1062_bases
ATGAAAAAAATTGGTTTCTTTATAATGAATATCGAAAGTGCGGGGGGAACGGAGCGCGTTTCTATTAATGTTGCTAATGC
TCTTGCTAAACAGGGATATGATGTTTCTTTTATTAGCATTGTCGGTAACAAGCCTTTTTTCCAAGTTGATGAAAAAATTA
ATATTTACGCAATGAATAAATTGCCCTATTCATTGAAAAAAGATTATTTTTCTATCACTAAAAAATTAAGAGAATTAGTT
AAAGAATTACAGCTGGATACCTTAATTGTGGTTGATGGTGCAATTATGCTTTTTTCTGCTTTGGCGTTGGTTAATTTAAA
TATAAAACATATTTTATGGGAGCATTATTCTTTTAATTTTACGGGCAATCGTCTCGTTCGCACGCTCGGTAAATATTTAG
CTGTAACAACTTGCGATAAAATAGTTACATTGACAGAAGCAGAAAAAACGCTGTGGCAAGAAAAATTTAAAACGAACAAT
ATAATCACTATTGCTAATCCAAATACGCTTTTGCCAAAGAACAAATTAGCAAAATGGGAAAATAAAACTATTTTATCTGT
AGGGCATTTATTTTCTTATAAAGGTTTTGATTATTTATTAAAAGTTTGGCAAGTTTTGGCAAAAAAATATCCAGATTGGA
ATTTAAAAATAGTGGGTTCTGGCGAGGAAGAAGAGAATTTAAAAAATCTTGCTAAAGCATTGGATATTGAAGATTCAGTT
AATTTTACTCCTCGTACTAATGATGTCTCTTTTTATTATGAAAGCAGTTCAATTTATTGTTTACCTTCACAAACAGAAGG
CTTGCCTTTAGTCGTTATTGAAGCGATGGCATTTGGCTTACCTATTGTTGCCTTTAATTGTTCTCCTGGCGTAAAGCAAT
TAGTGGAACATAAAGAAAATGGTTTTCTTTGTGAAAAAAATAATATTGAAGAAATGGTTAAAGGTTTAGATTTATTAATC
AATAATCCTGAACTTTATCAACAAATGTCAGACAAATCTCGTTTAATGAGTGAAGATTATGGCATTGAGAAAATTATTGA
AGAATGGAAAGGGATTTTATAA

Upstream 100 bases:

>100_bases
AATGTAGAAATTCGTTTTATTAGAACGAGTATTCCAAGATGGCAATTCTGTTACGATTCATTTTCAGACCTTGGATTAAC
GATATATAAGGAAATTTAAA

Downstream 100 bases:

>100_bases
GGAATAATTTTATGTTAAAAAAATATTTGATCTCTTTAGATAAAGATATTCAACGTCGGAAATTATTCTTTTCTCAAAAA
AATACCGAGGATTTCCAAAT

Product: putative UDP-galactose--lipooligosaccharide galactosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKKIGFFIMNIESAGGTERVSINVANALAKQGYDVSFISIVGNKPFFQVDEKINIYAMNKLPYSLKKDYFSITKKLRELV
KELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYSFNFTGNRLVRTLGKYLAVTTCDKIVTLTEAEKTLWQEKFKTNN
IITIANPNTLLPKNKLAKWENKTILSVGHLFSYKGFDYLLKVWQVLAKKYPDWNLKIVGSGEEEENLKNLAKALDIEDSV
NFTPRTNDVSFYYESSSIYCLPSQTEGLPLVVIEAMAFGLPIVAFNCSPGVKQLVEHKENGFLCEKNNIEEMVKGLDLLI
NNPELYQQMSDKSRLMSEDYGIEKIIEEWKGIL

Sequences:

>Translated_353_residues
MKKIGFFIMNIESAGGTERVSINVANALAKQGYDVSFISIVGNKPFFQVDEKINIYAMNKLPYSLKKDYFSITKKLRELV
KELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYSFNFTGNRLVRTLGKYLAVTTCDKIVTLTEAEKTLWQEKFKTNN
IITIANPNTLLPKNKLAKWENKTILSVGHLFSYKGFDYLLKVWQVLAKKYPDWNLKIVGSGEEEENLKNLAKALDIEDSV
NFTPRTNDVSFYYESSSIYCLPSQTEGLPLVVIEAMAFGLPIVAFNCSPGVKQLVEHKENGFLCEKNNIEEMVKGLDLLI
NNPELYQQMSDKSRLMSEDYGIEKIIEEWKGIL
>Mature_353_residues
MKKIGFFIMNIESAGGTERVSINVANALAKQGYDVSFISIVGNKPFFQVDEKINIYAMNKLPYSLKKDYFSITKKLRELV
KELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYSFNFTGNRLVRTLGKYLAVTTCDKIVTLTEAEKTLWQEKFKTNN
IITIANPNTLLPKNKLAKWENKTILSVGHLFSYKGFDYLLKVWQVLAKKYPDWNLKIVGSGEEEENLKNLAKALDIEDSV
NFTPRTNDVSFYYESSSIYCLPSQTEGLPLVVIEAMAFGLPIVAFNCSPGVKQLVEHKENGFLCEKNNIEEMVKGLDLLI
NNPELYQQMSDKSRLMSEDYGIEKIIEEWKGIL

Specific function: Adds A Galactose Goup To A Glucose Group Of Lps. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI226510984, Length=337, Percent_Identity=22.2551928783383, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788357, Length=134, Percent_Identity=29.8507462686567, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 40209; Mature: 40209

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGFFIMNIESAGGTERVSINVANALAKQGYDVSFISIVGNKPFFQVDEKINIYAMNK
CCCEEEEEEEEECCCCCEEEEEEHHHHHHHCCCCEEEEEEECCCCCEEECCCEEEEEECC
LPYSLKKDYFSITKKLRELVKELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYSFNF
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCHHHHEEEEEEECC
TGNRLVRTLGKYLAVTTCDKIVTLTEAEKTLWQEKFKTNNIITIANPNTLLPKNKLAKWE
CHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCC
NKTILSVGHLFSYKGFDYLLKVWQVLAKKYPDWNLKIVGSGEEEENLKNLAKALDIEDSV
CCEEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCC
NFTPRTNDVSFYYESSSIYCLPSQTEGLPLVVIEAMAFGLPIVAFNCSPGVKQLVEHKEN
CCCCCCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHCCC
GFLCEKNNIEEMVKGLDLLINNPELYQQMSDKSRLMSEDYGIEKIIEEWKGIL
CEEECCCCHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKKIGFFIMNIESAGGTERVSINVANALAKQGYDVSFISIVGNKPFFQVDEKINIYAMNK
CCCEEEEEEEEECCCCCEEEEEEHHHHHHHCCCCEEEEEEECCCCCEEECCCEEEEEECC
LPYSLKKDYFSITKKLRELVKELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYSFNF
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCHHHHEEEEEEECC
TGNRLVRTLGKYLAVTTCDKIVTLTEAEKTLWQEKFKTNNIITIANPNTLLPKNKLAKWE
CHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCC
NKTILSVGHLFSYKGFDYLLKVWQVLAKKYPDWNLKIVGSGEEEENLKNLAKALDIEDSV
CCEEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCC
NFTPRTNDVSFYYESSSIYCLPSQTEGLPLVVIEAMAFGLPIVAFNCSPGVKQLVEHKEN
CCCCCCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHCCC
GFLCEKNNIEEMVKGLDLLINNPELYQQMSDKSRLMSEDYGIEKIIEEWKGIL
CEEECCCCHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]