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The map label for this gene is dacA [H]

Identifier: 148825690

GI number: 148825690

Start: 635973

End: 637076

Strand: Direct

Name: dacA [H]

Synonym: CGSHiEE_03165

Alternate gene names: 148825690

Gene position: 635973-637076 (Clockwise)

Preceding gene: 148825689

Following gene: 148825691

Centisome position: 35.08

GC content: 35.33

Gene sequence:

>1104_bases
ATGCAGTTTGGCGTAACTCCACCACAAATTACGGCTCAAACGTATGTATTGATGGATTATAATTCAGGTGCAATTTTGAC
CGCACTTAATCCAGATCAACGTCAATATCCAGCATCACTGACTAAAATGATGACAAGTTATGTAGTTGGTGTCGCATTAA
AACAAGGCAAAATCCATAATACCGATATGGTTACCATTGGTGAAAGTGCGTGGGGGCGTAATTTCCCTGATTCATCAAAA
ATGTTTTTAGATTTAAATACACAAGTATCTGTAGCAGACTTAAATCGAGGTGTGATTGTTGTTTCTGGTAATGATGCGAC
TGTTGCATTAGCTGAACATATTTCTGGCAATGTACCGAACTTTGTAGAAACAATGAATAAATATGTTCAACAATTTGGTT
TGAAAAATACAAATTTCACCACGCCCCATGGTTTAGACGATCCAAATCAATATTCTTCCGCGCGTGATATGGCAATTATT
GGTGCACATATTATTCGTGATTTACCTGAAGAATATAAAATTTACTCCGAAAAAGACTTTACATTTAATAAAATCAAACA
AGCTAACCGTAATGGATTATTATGGGATAAAACTATCAATGTTGATGGAATGAAGACAGGGCATACTAGCCAAGCAGGAT
ATAACTTAGTCGCTTCAGCGACTACATCAAACAATATGCGTTTAATTTCTGTAGTAATGGGTGTGCCAACTTACAAAGGT
CGTGAAGTAGAAAGTAAGAAACTTTTACAATGGGGTTTTGCTAATTTCGAAACATTTAAAACTTTAGAGGCTGGTAAAGA
AATTTCAGAACAAAGGGTTTATTATGGCGATAAAAATTCAGTAAAACTTGGTGCTTTAATGGATCACTTTATTACGATCC
CTAAAGGTAAACAAAGTGAGGTTAAAGCTCGTTATGAATTAGCAGATAAAAATTTACAAGCACCATTAGCAAAAGGTCAA
GTGATTGGTAAAGTGGTTTATCAACTGGATGGTAAGGATATTGCAAGTGCCAATTTACAAGTGATGAATGATGTGGGAGA
AGCGGGTATTTTTGGTAAACTATGGGATTGGTTAGTATTGACTGTGAAAGGGTTATTTTCATAA

Upstream 100 bases:

>100_bases
AATTAATTGTAGGAACACACTATGTTGAAAAGAACAAACAAAAATCGCATTTTTATCAAGTTTTGTGGCACTTAGTGCTT
TTTCGGTATCTGCAGAGGAT

Downstream 100 bases:

>100_bases
AAAGAATTTGAAAATTGACCGCACTTTTTGATTTATTTTATTTTTAGGAAAGAAAATGACAATAGAAAACGATTATGCAA
AACTCAAGGAATTAATGGAA

Product: hypothetical protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQFGVTPPQITAQTYVLMDYNSGAILTALNPDQRQYPASLTKMMTSYVVGVALKQGKIHNTDMVTIGESAWGRNFPDSSK
MFLDLNTQVSVADLNRGVIVVSGNDATVALAEHISGNVPNFVETMNKYVQQFGLKNTNFTTPHGLDDPNQYSSARDMAII
GAHIIRDLPEEYKIYSEKDFTFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGYNLVASATTSNNMRLISVVMGVPTYKG
REVESKKLLQWGFANFETFKTLEAGKEISEQRVYYGDKNSVKLGALMDHFITIPKGKQSEVKARYELADKNLQAPLAKGQ
VIGKVVYQLDGKDIASANLQVMNDVGEAGIFGKLWDWLVLTVKGLFS

Sequences:

>Translated_367_residues
MQFGVTPPQITAQTYVLMDYNSGAILTALNPDQRQYPASLTKMMTSYVVGVALKQGKIHNTDMVTIGESAWGRNFPDSSK
MFLDLNTQVSVADLNRGVIVVSGNDATVALAEHISGNVPNFVETMNKYVQQFGLKNTNFTTPHGLDDPNQYSSARDMAII
GAHIIRDLPEEYKIYSEKDFTFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGYNLVASATTSNNMRLISVVMGVPTYKG
REVESKKLLQWGFANFETFKTLEAGKEISEQRVYYGDKNSVKLGALMDHFITIPKGKQSEVKARYELADKNLQAPLAKGQ
VIGKVVYQLDGKDIASANLQVMNDVGEAGIFGKLWDWLVLTVKGLFS
>Mature_367_residues
MQFGVTPPQITAQTYVLMDYNSGAILTALNPDQRQYPASLTKMMTSYVVGVALKQGKIHNTDMVTIGESAWGRNFPDSSK
MFLDLNTQVSVADLNRGVIVVSGNDATVALAEHISGNVPNFVETMNKYVQQFGLKNTNFTTPHGLDDPNQYSSARDMAII
GAHIIRDLPEEYKIYSEKDFTFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGYNLVASATTSNNMRLISVVMGVPTYKG
REVESKKLLQWGFANFETFKTLEAGKEISEQRVYYGDKNSVKLGALMDHFITIPKGKQSEVKARYELADKNLQAPLAKGQ
VIGKVVYQLDGKDIASANLQVMNDVGEAGIFGKLWDWLVLTVKGLFS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=362, Percent_Identity=54.6961325966851, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1787062, Length=365, Percent_Identity=52.6027397260274, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI87082022, Length=359, Percent_Identity=48.7465181058496, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI87082054, Length=256, Percent_Identity=26.5625, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 40585; Mature: 40585

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFGVTPPQITAQTYVLMDYNSGAILTALNPDQRQYPASLTKMMTSYVVGVALKQGKIHN
CCCCCCCCCCCEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TDMVTIGESAWGRNFPDSSKMFLDLNTQVSVADLNRGVIVVSGNDATVALAEHISGNVPN
CCEEEECCHHCCCCCCCCCCEEEEECCEEEEEECCCCEEEEECCCCEEEEEHHHCCCCCH
FVETMNKYVQQFGLKNTNFTTPHGLDDPNQYSSARDMAIIGAHIIRDLPEEYKIYSEKDF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCHHHEECCCCCC
TFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGYNLVASATTSNNMRLISVVMGVPTYKG
CHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCC
REVESKKLLQWGFANFETFKTLEAGKEISEQRVYYGDKNSVKLGALMDHFITIPKGKQSE
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCCEEHHHHHHHHEECCCCCCHH
VKARYELADKNLQAPLAKGQVIGKVVYQLDGKDIASANLQVMNDVGEAGIFGKLWDWLVL
HHHHHHHHCCCCCCCHHHHHHHEEHHEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
TVKGLFS
HHHHHCC
>Mature Secondary Structure
MQFGVTPPQITAQTYVLMDYNSGAILTALNPDQRQYPASLTKMMTSYVVGVALKQGKIHN
CCCCCCCCCCCEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TDMVTIGESAWGRNFPDSSKMFLDLNTQVSVADLNRGVIVVSGNDATVALAEHISGNVPN
CCEEEECCHHCCCCCCCCCCEEEEECCEEEEEECCCCEEEEECCCCEEEEEHHHCCCCCH
FVETMNKYVQQFGLKNTNFTTPHGLDDPNQYSSARDMAIIGAHIIRDLPEEYKIYSEKDF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCHHHEECCCCCC
TFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGYNLVASATTSNNMRLISVVMGVPTYKG
CHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCC
REVESKKLLQWGFANFETFKTLEAGKEISEQRVYYGDKNSVKLGALMDHFITIPKGKQSE
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCCEEHHHHHHHHEECCCCCCHH
VKARYELADKNLQAPLAKGQVIGKVVYQLDGKDIASANLQVMNDVGEAGIFGKLWDWLVL
HHHHHHHHCCCCCCCHHHHHHHEEHHEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
TVKGLFS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]