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The map label for this gene is rlmH [H]

Identifier: 148825686

GI number: 148825686

Start: 631413

End: 631880

Strand: Direct

Name: rlmH [H]

Synonym: CGSHiEE_03145

Alternate gene names: 148825686

Gene position: 631413-631880 (Clockwise)

Preceding gene: 148825685

Following gene: 148825687

Centisome position: 34.83

GC content: 45.3

Gene sequence:

>468_bases
GTGAAAATCACGTTAATTGCCGTCGGAACAAAAATGCCTTCTTGGGTTACAACGGGTTTTGAGGAATATCAACGCCGTTT
CCCAAAAGATATGCCTTTTGAACTAATTGAAATCCCAGCAGGCAAGCGTGGAAAAAATGCTGATATTAAACGTATTTTAG
AACAAGAAGGTAAAGCGATGTTAGCGGCCTGTGGAAAAGGCAAAGTCGTGACGTTAGATATTCCTGGTAAACCTTGGACG
ACACCGCAGCTAGCTGAGCAACTAGAAGCGTGGAAAAATGATGGTCGCGATGTTTGTTTATTGATTGGTGGGCCTGAGGG
GCTTTCGCCAGAATGCAAAGCTGTCGCAGAGCAAAGTTGGTCGCTTTCTCCCTTGACATTACCTCACCCGCTTGTTCGTG
TCGTGGTGGCTGAAAGTTTGTATCGTGCGTGGTCGCTAACCACTAATCATCCTTATCATCGAGAATAA

Upstream 100 bases:

>100_bases
AACGTGACGCCCGTGAAATGTATCAATTAGAGAAACTTTGGGCGTAAATTTTAGTAAAGTGCGGTAAAAAATAACCGCAC
TTTTTAGATAAGGATGACTT

Downstream 100 bases:

>100_bases
TATATCCTTATTTTTAGTCAGATGAATTTAAAAAAATTCTTCAATACACCAACGCACGAACCGTTCCGCGATAAAAAAGC
GGAGCGTAACCTTTTTGCAC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKITLIAVGTKMPSWVTTGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAMLAACGKGKVVTLDIPGKPWT
TPQLAEQLEAWKNDGRDVCLLIGGPEGLSPECKAVAEQSWSLSPLTLPHPLVRVVVAESLYRAWSLTTNHPYHRE

Sequences:

>Translated_155_residues
MKITLIAVGTKMPSWVTTGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAMLAACGKGKVVTLDIPGKPWT
TPQLAEQLEAWKNDGRDVCLLIGGPEGLSPECKAVAEQSWSLSPLTLPHPLVRVVVAESLYRAWSLTTNHPYHRE
>Mature_155_residues
MKITLIAVGTKMPSWVTTGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAMLAACGKGKVVTLDIPGKPWT
TPQLAEQLEAWKNDGRDVCLLIGGPEGLSPECKAVAEQSWSLSPLTLPHPLVRVVVAESLYRAWSLTTNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=83.2258064516129, Blast_Score=272, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016051
- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 17278; Mature: 17278

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITLIAVGTKMPSWVTTGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAM
CEEEEEEECCCCCHHHHHCHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEE
LAACGKGKVVTLDIPGKPWTTPQLAEQLEAWKNDGRDVCLLIGGPEGLSPECKAVAEQSW
EEECCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCC
SLSPLTLPHPLVRVVVAESLYRAWSLTTNHPYHRE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKITLIAVGTKMPSWVTTGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAM
CEEEEEEECCCCCHHHHHCHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEE
LAACGKGKVVTLDIPGKPWTTPQLAEQLEAWKNDGRDVCLLIGGPEGLSPECKAVAEQSW
EEECCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCC
SLSPLTLPHPLVRVVVAESLYRAWSLTTNHPYHRE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA