Click here to switch to the map view.

The map label for this gene is rlmL [H]

Identifier: 148825609

GI number: 148825609

Start: 529827

End: 531962

Strand: Direct

Name: rlmL [H]

Synonym: CGSHiEE_02695

Alternate gene names: 148825609

Gene position: 529827-531962 (Clockwise)

Preceding gene: 148825605

Following gene: 148825610

Centisome position: 29.22

GC content: 37.08

Gene sequence:

>2136_bases
ATGAAACAATTATTTGCAACGACTTCTCGTGGCTTTGAAGAATTATTAAAAGTTGAACTCACTGAACTTGGCGCGCAAGA
GGCAAAAGTGGTTCAAGGTGGCGTTCATTATCAAGCTGATGACGAAACTTTATACCGCACTTTGCTTTGGTCACGTCTTG
CTTCACGCATTTTATTTCCTTTGATTGAAACCAAAATTTACAGTGATTTGGATCTTTATGCGGCTGTTTCAGGCTTTAAT
TGGTTAGCGCAATTTGATGAACGAGTTACCTTCTTTGTCGATTTTAATGGAACCAACCAAGAAATTCGTCATACTCAATT
TGGTGCGATGCGCGTTAAAGATGGCATTGTCGATTACTTTGAACGTCAAGGTAAAACTCGCCCTGATGTAGATAAAATTC
AGCCAGACGTACGTATCCATGCTTATTTGAATCGTGAAAATTTAGTGATTTCTTTAGATTTAAGTGGGGAAGCATTACAT
TTGCGAGGCTATCGTGAAGATGCAGGTCAAGCACCTTTACGTGAAACTCTAGCGGCGGCAATTGTTATGCGTTCAGGTTG
GCAAGTGGGTTCTCCATTAGTAGATCCAATGTGTGGTTCAGGTACGCTTTTAATTGAAGCAGCACAAATAGAAGCTAAAA
TTGCACCGCAACTTTATCGTTTACATTGGGGATTTGATTTTTGGAAAGCGCATAATCAATCGGCTTGGGAAAAAGTAAAA
AATGAAGCGATTGAATTAGCAGAAGAAAAACAAAGTGAAATTCAACCGCACTTTTATGGATTTGACCTCGATCACCGCGT
ATTAAAAAAGGCGCAGAAAAATGCACAAAATGGAGGTGTTTCACATTTAATTAAATGGCAGCAAGCTGATGTGGCAGCGT
TGAAAAATCCTCGTTTAAATGAAGTTGGTACGGTTATCTGTAATCCACCTTATGGCGAACGTTTAGGAACAACACCAGCT
TTAATTGCGCTCTATTCTGTATTTGGGCAAAGACTTAAAAAAGAATTTTGTGGTTGGAATGTTTCTGTTTTTAGTAGCGA
ATCCACATTGCTTGATTGTTTGAGAATGCGTGCCAGCCGACAGTTTAAAGCAAAAAATGGCCCACTAGATTGTGTGCAAA
AGAATTATCAAGTTTCAGAACGAAAATCTGACGCAATTACAGATGAAAAGGAACTAGAATTTAACCGCACTTCGGAGGTC
GCCCCCGATTTTGCTAATCGATTACAAAAAAATATTAAGAAAATTAGCAAATGGGCAAAGCAACAAGAATTAGACGCTTA
TCGTTTATATGATGCTGATTTGCCAGAATATAATTTAGCTGTTGATCGTTATGCAGATTACATAGTTGTACAAGAATATG
CTGCGCCGAAAAATATTGATGAAAATAAAGCGCGTCAGCGTTTGTTAGATGCCGTTACTGCAACTTTGCAAGTGACAGGT
GTGGAAACAAATAAGTTGATCCTCAAAGTTCGTCAGAAACAAAAAGGTACAAATCAATATGAGAAGTTAGCCAATAAAGG
CGAATACTTTTATGTGAATGAATATGGCGCGCAACTTTGGGTAAATTTGACAGATTATTTAGACACTGGATTATTCTTAG
ATCACCGTTTAACAAGAAAAATGATTGGTGAGTTGGCAAAGGGTAAAGATTTCTTGAACTTATTTGCTTATACAGGTTCT
GCCACTGTTCATGCAGCACTTGGTGGCGCTAAATCGACAACTACAGTTGATATGTCCAATACTTATCTTAATTGGGCGGA
GCAGAATCTTATTTTGAATGATATTGAAGGTAAACAACATAAATTAATTCAAGCAGATTGCCTTCAATGGTTAGAAAAAT
GTGATCGTCAATTTGATTTGATATTTGTGGATCCACCGACGTTTTCAAATTCTAAACGTATGGAAGAGAGTTGGGATGTT
CAACGAGATCATGTGAAATTGATGAGCAACCTAAAACGAGTTTTGTCGAATAATGGCACAATTGTTTTTTCGAATAATAA
ACGTGGATTCAAAATGAATTTAGTTGCGTTGGAAGAATTAGGTTTAAGTGCAATAGAAATTTCGCATAAAACCTTACCGC
TAGATTTTGAACGTAATAAACAAATTCATAATTGTTGGATGATTCAACATATATAA

Upstream 100 bases:

>100_bases
CGGGGCTTTTCTTTTTACAGTTTAACTCGCTATGATGATCTCAATTAGATCATCTTTTTCTATGCTATAATCCCTCAAAA
TTTTCAAAATCGGTACCTAA

Downstream 100 bases:

>100_bases
TGATTTACTAAGTTGTAAAAATAGTCGAAAAAATAGTGCATTTTAAACAAACTGAATAAAAAACTATCGCTCAAACATTT
TTTTTCAAAAAGTGCTAGAC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MKQLFATTSRGFEELLKVELTELGAQEAKVVQGGVHYQADDETLYRTLLWSRLASRILFPLIETKIYSDLDLYAAVSGFN
WLAQFDERVTFFVDFNGTNQEIRHTQFGAMRVKDGIVDYFERQGKTRPDVDKIQPDVRIHAYLNRENLVISLDLSGEALH
LRGYREDAGQAPLRETLAAAIVMRSGWQVGSPLVDPMCGSGTLLIEAAQIEAKIAPQLYRLHWGFDFWKAHNQSAWEKVK
NEAIELAEEKQSEIQPHFYGFDLDHRVLKKAQKNAQNGGVSHLIKWQQADVAALKNPRLNEVGTVICNPPYGERLGTTPA
LIALYSVFGQRLKKEFCGWNVSVFSSESTLLDCLRMRASRQFKAKNGPLDCVQKNYQVSERKSDAITDEKELEFNRTSEV
APDFANRLQKNIKKISKWAKQQELDAYRLYDADLPEYNLAVDRYADYIVVQEYAAPKNIDENKARQRLLDAVTATLQVTG
VETNKLILKVRQKQKGTNQYEKLANKGEYFYVNEYGAQLWVNLTDYLDTGLFLDHRLTRKMIGELAKGKDFLNLFAYTGS
ATVHAALGGAKSTTTVDMSNTYLNWAEQNLILNDIEGKQHKLIQADCLQWLEKCDRQFDLIFVDPPTFSNSKRMEESWDV
QRDHVKLMSNLKRVLSNNGTIVFSNNKRGFKMNLVALEELGLSAIEISHKTLPLDFERNKQIHNCWMIQHI

Sequences:

>Translated_711_residues
MKQLFATTSRGFEELLKVELTELGAQEAKVVQGGVHYQADDETLYRTLLWSRLASRILFPLIETKIYSDLDLYAAVSGFN
WLAQFDERVTFFVDFNGTNQEIRHTQFGAMRVKDGIVDYFERQGKTRPDVDKIQPDVRIHAYLNRENLVISLDLSGEALH
LRGYREDAGQAPLRETLAAAIVMRSGWQVGSPLVDPMCGSGTLLIEAAQIEAKIAPQLYRLHWGFDFWKAHNQSAWEKVK
NEAIELAEEKQSEIQPHFYGFDLDHRVLKKAQKNAQNGGVSHLIKWQQADVAALKNPRLNEVGTVICNPPYGERLGTTPA
LIALYSVFGQRLKKEFCGWNVSVFSSESTLLDCLRMRASRQFKAKNGPLDCVQKNYQVSERKSDAITDEKELEFNRTSEV
APDFANRLQKNIKKISKWAKQQELDAYRLYDADLPEYNLAVDRYADYIVVQEYAAPKNIDENKARQRLLDAVTATLQVTG
VETNKLILKVRQKQKGTNQYEKLANKGEYFYVNEYGAQLWVNLTDYLDTGLFLDHRLTRKMIGELAKGKDFLNLFAYTGS
ATVHAALGGAKSTTTVDMSNTYLNWAEQNLILNDIEGKQHKLIQADCLQWLEKCDRQFDLIFVDPPTFSNSKRMEESWDV
QRDHVKLMSNLKRVLSNNGTIVFSNNKRGFKMNLVALEELGLSAIEISHKTLPLDFERNKQIHNCWMIQHI
>Mature_711_residues
MKQLFATTSRGFEELLKVELTELGAQEAKVVQGGVHYQADDETLYRTLLWSRLASRILFPLIETKIYSDLDLYAAVSGFN
WLAQFDERVTFFVDFNGTNQEIRHTQFGAMRVKDGIVDYFERQGKTRPDVDKIQPDVRIHAYLNRENLVISLDLSGEALH
LRGYREDAGQAPLRETLAAAIVMRSGWQVGSPLVDPMCGSGTLLIEAAQIEAKIAPQLYRLHWGFDFWKAHNQSAWEKVK
NEAIELAEEKQSEIQPHFYGFDLDHRVLKKAQKNAQNGGVSHLIKWQQADVAALKNPRLNEVGTVICNPPYGERLGTTPA
LIALYSVFGQRLKKEFCGWNVSVFSSESTLLDCLRMRASRQFKAKNGPLDCVQKNYQVSERKSDAITDEKELEFNRTSEV
APDFANRLQKNIKKISKWAKQQELDAYRLYDADLPEYNLAVDRYADYIVVQEYAAPKNIDENKARQRLLDAVTATLQVTG
VETNKLILKVRQKQKGTNQYEKLANKGEYFYVNEYGAQLWVNLTDYLDTGLFLDHRLTRKMIGELAKGKDFLNLFAYTGS
ATVHAALGGAKSTTTVDMSNTYLNWAEQNLILNDIEGKQHKLIQADCLQWLEKCDRQFDLIFVDPPTFSNSKRMEESWDV
QRDHVKLMSNLKRVLSNNGTIVFSNNKRGFKMNLVALEELGLSAIEISHKTLPLDFERNKQIHNCWMIQHI

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=709, Percent_Identity=60.0846262341326, Blast_Score=895, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=240, Percent_Identity=29.5833333333333, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 81330; Mature: 81330

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLFATTSRGFEELLKVELTELGAQEAKVVQGGVHYQADDETLYRTLLWSRLASRILFP
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHH
LIETKIYSDLDLYAAVSGFNWLAQFDERVTFFVDFNGTNQEIRHTQFGAMRVKDGIVDYF
HHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHH
ERQGKTRPDVDKIQPDVRIHAYLNRENLVISLDLSGEALHLRGYREDAGQAPLRETLAAA
HHCCCCCCCHHHCCCCEEEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHH
IVMRSGWQVGSPLVDPMCGSGTLLIEAAQIEAKIAPQLYRLHWGFDFWKAHNQSAWEKVK
HHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHCHHHHHHHCCCHHHHHCCHHHHHHHH
NEAIELAEEKQSEIQPHFYGFDLDHRVLKKAQKNAQNGGVSHLIKWQQADVAALKNPRLN
HHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
EVGTVICNPPYGERLGTTPALIALYSVFGQRLKKEFCGWNVSVFSSESTLLDCLRMRASR
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHC
QFKAKNGPLDCVQKNYQVSERKSDAITDEKELEFNRTSEVAPDFANRLQKNIKKISKWAK
CCCCCCCCHHHHHCCCCHHHCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QQELDAYRLYDADLPEYNLAVDRYADYIVVQEYAAPKNIDENKARQRLLDAVTATLQVTG
HHCCCCEEEECCCCCCCCEEHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEEC
VETNKLILKVRQKQKGTNQYEKLANKGEYFYVNEYGAQLWVNLTDYLDTGLFLDHRLTRK
CCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHH
MIGELAKGKDFLNLFAYTGSATVHAALGGAKSTTTVDMSNTYLNWAEQNLILNDIEGKQH
HHHHHHCCCHHHHHEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHCCCEEECCCCCHH
KLIQADCLQWLEKCDRQFDLIFVDPPTFSNSKRMEESWDVQRDHVKLMSNLKRVLSNNGT
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCE
IVFSNNKRGFKMNLVALEELGLSAIEISHKTLPLDFERNKQIHNCWMIQHI
EEEECCCCCEEEEEEEHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKQLFATTSRGFEELLKVELTELGAQEAKVVQGGVHYQADDETLYRTLLWSRLASRILFP
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHH
LIETKIYSDLDLYAAVSGFNWLAQFDERVTFFVDFNGTNQEIRHTQFGAMRVKDGIVDYF
HHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHH
ERQGKTRPDVDKIQPDVRIHAYLNRENLVISLDLSGEALHLRGYREDAGQAPLRETLAAA
HHCCCCCCCHHHCCCCEEEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHH
IVMRSGWQVGSPLVDPMCGSGTLLIEAAQIEAKIAPQLYRLHWGFDFWKAHNQSAWEKVK
HHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHCHHHHHHHCCCHHHHHCCHHHHHHHH
NEAIELAEEKQSEIQPHFYGFDLDHRVLKKAQKNAQNGGVSHLIKWQQADVAALKNPRLN
HHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
EVGTVICNPPYGERLGTTPALIALYSVFGQRLKKEFCGWNVSVFSSESTLLDCLRMRASR
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHC
QFKAKNGPLDCVQKNYQVSERKSDAITDEKELEFNRTSEVAPDFANRLQKNIKKISKWAK
CCCCCCCCHHHHHCCCCHHHCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QQELDAYRLYDADLPEYNLAVDRYADYIVVQEYAAPKNIDENKARQRLLDAVTATLQVTG
HHCCCCEEEECCCCCCCCEEHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEEC
VETNKLILKVRQKQKGTNQYEKLANKGEYFYVNEYGAQLWVNLTDYLDTGLFLDHRLTRK
CCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHH
MIGELAKGKDFLNLFAYTGSATVHAALGGAKSTTTVDMSNTYLNWAEQNLILNDIEGKQH
HHHHHHCCCHHHHHEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHCCCEEECCCCCHH
KLIQADCLQWLEKCDRQFDLIFVDPPTFSNSKRMEESWDVQRDHVKLMSNLKRVLSNNGT
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCE
IVFSNNKRGFKMNLVALEELGLSAIEISHKTLPLDFERNKQIHNCWMIQHI
EEEECCCCCEEEEEEEHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA