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The map label for this gene is mpl [H]

Identifier: 148825604

GI number: 148825604

Start: 524344

End: 525705

Strand: Direct

Name: mpl [H]

Synonym: CGSHiEE_02670

Alternate gene names: 148825604

Gene position: 524344-525705 (Clockwise)

Preceding gene: 148825602

Following gene: 148825605

Centisome position: 28.92

GC content: 39.5

Gene sequence:

>1362_bases
ATGAAACATATTCATATCTTAGGTATTTGTGGCACATTTATGGGCGGTGTGGCGATGATTGCTAAACAAATGGGCTATCA
CGTAACGGGTTCCGATACTAATGTTTACCCTCCAATGAGCACTTTTTTAGAAGAGCAAGGTATCGAGATTATTCCAAATT
ATGATGTTGCACAATTACAACCTGCGCCTGATATGGTAATTGTTGGTAATGCGATGAAGCGTGGTAATCCTTGCGTAGAA
TATGTGTTGGAAAATAATTTGAAATATACATCTGGCCCTCAATGGTTACATGATCATTTATTGCGAGATCGTTGGGTATT
GGCGGTTTCAGGTACGCATGGAAAAACAACGACAACAGGTATGCTAACGTGGATCTTAGAACAAAATGGCTTAAAACCAG
GTTTTTTAATCGGAGGAATTGCGGGTAATTTTGGCATTTCTGCCCGTTTAGGCACAAGTCCTTATTTTGTTATTGAAGCT
GATGAATATGATACAGCTTTTTTTGATAAACGTTCTAAATTTATCCATTACAATCCACGTACACTTATCGTAAATAACAT
TAGTTTTGACCATGCAGATATTTTTGATGATCTCAAAGCGATCCAACGCCAATTTCATCATATGATTCGAACCATTCCAG
CAAGTGGCTTGGTATTAAGTTCGGCGAGTGAACAAAGTGCAAAAGAAACTTTGGCATTGGGATGTTGGTCTCAACAGCAA
TTTTTAGGCAAAGATAACGAGTGGTTTGCTGAGCGCATTACAAATGATGCTTCACATTTTGCGGTTTTCCATCATGGAGA
AAAAGTGGCAGAAGTAAAATGGAATGTAGTGGGACAACATAATATGCATAATGCTTTAATGGCGATCGCTGCGGCTCATC
ATACTGGCGTTGCTATTGAAGATGCGTGTAAAGCATTGGGATCTTTTGTGAATGCTAAACGTCGTTTAGAAGTGAAAGGC
GAAGTTAATAGCATTACGGTTTATGATGATTTTGCGCATCATCCAGAAGCTATTTTGGCGACTTTAACTGCATTGCGCGA
TAAAGTGGGTGGTGGTGTTCGTATTCTTGCAGTGCTTGAACCTCGTTCTAACACAATGAAAATGGGGGTTCATAAAGATG
AAATTGCGCCTGCACTTGGCAGAGCTGATTCCGTGTTTATGCTCCAACCTGAACAACTTCCTTGGGAAGTGGCAGATATT
GCAAACCAATGTGTACAACCTGCTTATTGGAATGCAAATTTAGATCGATTAGTTGATATGATTGTCGCTGAGGCCCAACC
TACAGATCACATTTTGGTGATGTCTAACGGCAGTTTTGGTGGCATTCATCAAAAAATTCTTGATAAGCTTAAACAGAAAT
AA

Upstream 100 bases:

>100_bases
TTATAGATCATATAGTTTGGTATGGCAGTCTTTTCTTTTGAAAGTTCATAGAAGTTGTTTTATCATATTTTTTGTTTTCC
TTAATCAACAGAGAAATACA

Downstream 100 bases:

>100_bases
TAAAAGTGCGGTTAATTTAGAACCTGTTTTTAAATTGACTGCACTTTTTATTTCTGACTTTTAGGCTATATTTATTTTTA
ATTTGTAATTCATAAAAAGG

Product: UDP-N-acetylmuramoylalanine-D-glutamate ligase-like protein

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQPAPDMVIVGNAMKRGNPCVE
YVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTGMLTWILEQNGLKPGFLIGGIAGNFGISARLGTSPYFVIEA
DEYDTAFFDKRSKFIHYNPRTLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIEDACKALGSFVNAKRRLEVKG
EVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLEPRSNTMKMGVHKDEIAPALGRADSVFMLQPEQLPWEVADI
ANQCVQPAYWNANLDRLVDMIVAEAQPTDHILVMSNGSFGGIHQKILDKLKQK

Sequences:

>Translated_453_residues
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQPAPDMVIVGNAMKRGNPCVE
YVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTGMLTWILEQNGLKPGFLIGGIAGNFGISARLGTSPYFVIEA
DEYDTAFFDKRSKFIHYNPRTLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIEDACKALGSFVNAKRRLEVKG
EVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLEPRSNTMKMGVHKDEIAPALGRADSVFMLQPEQLPWEVADI
ANQCVQPAYWNANLDRLVDMIVAEAQPTDHILVMSNGSFGGIHQKILDKLKQK
>Mature_453_residues
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQPAPDMVIVGNAMKRGNPCVE
YVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTGMLTWILEQNGLKPGFLIGGIAGNFGISARLGTSPYFVIEA
DEYDTAFFDKRSKFIHYNPRTLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIEDACKALGSFVNAKRRLEVKG
EVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLEPRSNTMKMGVHKDEIAPALGRADSVFMLQPEQLPWEVADI
ANQCVQPAYWNANLDRLVDMIVAEAQPTDHILVMSNGSFGGIHQKILDKLKQK

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=450, Percent_Identity=68.6666666666667, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=483, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50141; Mature: 50141

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQ
CCEEEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCEEECCCCHHEEC
PAPDMVIVGNAMKRGNPCVEYVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTG
CCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHH
MLTWILEQNGLKPGFLIGGIAGNFGISARLGTSPYFVIEADEYDTAFFDKRSKFIHYNPR
HEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCEEEEECCCCCCHHHHCCCCEEEECCC
TLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEEECCCHHH
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIE
HCCCCCHHHHHHHCCCCCEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHH
DACKALGSFVNAKRRLEVKGEVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLE
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEC
PRSNTMKMGVHKDEIAPALGRADSVFMLQPEQLPWEVADIANQCVQPAYWNANLDRLVDM
CCCCCEEECCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
IVAEAQPTDHILVMSNGSFGGIHQKILDKLKQK
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQ
CCEEEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCEEECCCCHHEEC
PAPDMVIVGNAMKRGNPCVEYVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTG
CCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHH
MLTWILEQNGLKPGFLIGGIAGNFGISARLGTSPYFVIEADEYDTAFFDKRSKFIHYNPR
HEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCEEEEECCCCCCHHHHCCCCEEEECCC
TLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEEECCCHHH
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIE
HCCCCCHHHHHHHCCCCCEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHH
DACKALGSFVNAKRRLEVKGEVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLE
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEC
PRSNTMKMGVHKDEIAPALGRADSVFMLQPEQLPWEVADIANQCVQPAYWNANLDRLVDM
CCCCCEEECCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
IVAEAQPTDHILVMSNGSFGGIHQKILDKLKQK
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]