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The map label for this gene is ppa

Identifier: 148825599

GI number: 148825599

Start: 520752

End: 521282

Strand: Direct

Name: ppa

Synonym: CGSHiEE_02645

Alternate gene names: 148825599

Gene position: 520752-521282 (Clockwise)

Preceding gene: 148825596

Following gene: 148825602

Centisome position: 28.72

GC content: 37.48

Gene sequence:

>531_bases
ATGGCTGATTTTAATCAAATTTTAACGCCAGGCGATGTAGATGCTGGCATTATCAATGTAGTAAATGAAATTCCCGAAGG
TAGTTGTCATAAAATCGAATGGAACCGTAAAGTTGCGGCATTCCAATTAGATCGTGTTGAGCCGGCAATTTTTGCAAAAC
CAACTAACTATGGTTTCATTCCACAAACTTTAGATGAAGATGGCGATGAGTTAGATGTGTTATTAATCACTCGCCAACCA
TTAGCAACAGGTGTATTCCTTGAGGCTAAAGTAATTGGTGTCATGAAATTTGTTGATGATGGTGAAGTGGATGATAAAAT
CGTTTGTGTTCCTGCAGATGATCGTGATACAGGCAATGCGTACAATTCATTAGCAGATTTACCGGCAAACTTAATTAAAC
AAATTGAGTTCCATTTCAATAACTATAAAGCACTTAAAAAACCTGGTTCAACGAAAGTAACTCATTGGGGCGATGTAGAA
GAAGCGAAAGAAGTGATTCGTGAGTCTATTAAACGTTGGAATGAGCGTTAA

Upstream 100 bases:

>100_bases
TAATCTATTTGCTGATTTTCTCTACAAATGAGACTGCGAGTCAGCAGAAAAACTGCTATACTTGTTGCGTTTTTTAACTT
GATAAAATGAAGGAAACAAA

Downstream 100 bases:

>100_bases
TTAAAGACGTTTGATTGTAAAAGTCCCTGATTCTTTGAGTCGGGGATTTTTTATATGAGTTTCAGAGATTTAGATAATAA
AAAAACACCTTTCGGTGCTT

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MADFNQILTPGDVDAGIINVVNEIPEGSCHKIEWNRKVAAFQLDRVEPAIFAKPTNYGFIPQTLDEDGDELDVLLITRQP
LATGVFLEAKVIGVMKFVDDGEVDDKIVCVPADDRDTGNAYNSLADLPANLIKQIEFHFNNYKALKKPGSTKVTHWGDVE
EAKEVIRESIKRWNER

Sequences:

>Translated_176_residues
MADFNQILTPGDVDAGIINVVNEIPEGSCHKIEWNRKVAAFQLDRVEPAIFAKPTNYGFIPQTLDEDGDELDVLLITRQP
LATGVFLEAKVIGVMKFVDDGEVDDKIVCVPADDRDTGNAYNSLADLPANLIKQIEFHFNNYKALKKPGSTKVTHWGDVE
EAKEVIRESIKRWNER
>Mature_175_residues
ADFNQILTPGDVDAGIINVVNEIPEGSCHKIEWNRKVAAFQLDRVEPAIFAKPTNYGFIPQTLDEDGDELDVLLITRQPL
ATGVFLEAKVIGVMKFVDDGEVDDKIVCVPADDRDTGNAYNSLADLPANLIKQIEFHFNNYKALKKPGSTKVTHWGDVEE
AKEVIRESIKRWNER

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family

Homologues:

Organism=Escherichia coli, GI1790673, Length=159, Percent_Identity=36.4779874213836, Blast_Score=82, Evalue=1e-17,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): IPYR_HAEIN (P44529)

Other databases:

- EMBL:   L42023
- PIR:   G64049
- RefSeq:   NP_438296.1
- ProteinModelPortal:   P44529
- SMR:   P44529
- GeneID:   951032
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0124
- TIGR:   HI_0124
- HOGENOM:   HBG529150
- OMA:   KKPGTCT
- ProtClustDB:   CLSK877664
- BioCyc:   HINF71421:HI_0124-MONOMER
- BRENDA:   3.6.1.1
- GO:   GO:0005737
- HAMAP:   MF_00209
- InterPro:   IPR008162
- Gene3D:   G3DSA:3.90.80.10
- PANTHER:   PTHR10286

Pfam domain/function: PF00719 Pyrophosphatase; SSF50324 Pyrophosphatase

EC number: =3.6.1.1

Molecular weight: Translated: 19725; Mature: 19594

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFNQILTPGDVDAGIINVVNEIPEGSCHKIEWNRKVAAFQLDRVEPAIFAKPTNYGFI
CCCCHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCCEEEEEEECCCCCEEEECCCCCCCC
PQTLDEDGDELDVLLITRQPLATGVFLEAKVIGVMKFVDDGEVDDKIVCVPADDRDTGNA
CCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHH
YNSLADLPANLIKQIEFHFNNYKALKKPGSTKVTHWGDVEEAKEVIRESIKRWNER
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADFNQILTPGDVDAGIINVVNEIPEGSCHKIEWNRKVAAFQLDRVEPAIFAKPTNYGFI
CCCHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCCEEEEEEECCCCCEEEECCCCCCCC
PQTLDEDGDELDVLLITRQPLATGVFLEAKVIGVMKFVDDGEVDDKIVCVPADDRDTGNA
CCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHH
YNSLADLPANLIKQIEFHFNNYKALKKPGSTKVTHWGDVEEAKEVIRESIKRWNER
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800